LOCUS       Guide-Generating_Vector 2764 bp ds-DNA     circular     30-SEP-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        28..157
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(22..27)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1040..1045
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(2001..2764)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     terminator      complement(1127..1235)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_RNA        1047..1126
                     /label="sgRNA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1..20
                     /label="Csy4 Site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             158..193
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1896..2000)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      911..1039
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1047..1050
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1236..1895)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     CDS             194..910
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gttcactgcc gtataggcag tgagacggaa agtgaaacgt gatttcatgc gtcattttga
       61 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
      121 ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga
      181 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
      241 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
      301 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc
      361 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
      421 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
      481 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
      541 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat
      601 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
      661 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
      721 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
      781 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
      841 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
      901 gtacaaatga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1021 gggcctttct gcgtttatac gtctcagttt tagagctaga aatagcaagt taaaataagg
     1081 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttacca ataaaaaacg
     1141 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1201 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1261 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1321 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1381 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1441 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     1501 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1561 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1621 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1681 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1741 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1801 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1861 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1921 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1981 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2701 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2761 ccgt
//