LOCUS       pAK-EL-02               2580 bp    DNA     circular     08-APR-2019
DEFINITION  Endlinker for tRNA system.
ACCESSION   urn.local...1cq-abnc7t5
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gRNA_Scaffold   409..448
                     /created_by="hahnf"
                     /label="sgRNA backbone (part)"
     overhang        409..412
                     /created_by="koroleva"
                     /label="AGTC"
     terminator      449..460
                     /created_by="hahnf"
                     /modified_by="hahnf"
                     /label="Poly-T terminator"
     overhang        461..464
                     /created_by="koroleva"
                     /label="CGCT"
     rep_origin      complement(877..1491)
                     /vntifkey=21
                     /label="rep(pMB1)"
     CDS             complement(1658..2467)
                     /vntifkey=21
                     /label="KanR"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cggtctcaag tccgttatca
      421 acttgaaaaa gtggcaccga gtcggtgctt tttttttttt cgcttgagac caagcttggt
      481 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      541 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      601 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      661 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      721 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      781 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      841 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1321 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1501 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1561 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaagccc
     1621 aatctgaata atgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     1681 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     1741 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     1801 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     1861 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     1921 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     1981 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     2041 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     2101 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     2161 tttttccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     2221 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     2281 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2341 catacaagcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     2401 catataaatc agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat
     2461 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     2521 atatattttt atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgca
//
