LOCUS       pAK-EL-05               2559 bp    DNA     circular     08-APR-2019
DEFINITION  Endlinker for tRNA system.
ACCESSION   urn.local...1ct-abnc7t5
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gRNA_Scaffold   409..427
                     /created_by="hahnf"
                     /label="sgRNA backbone (part)"
     overhang        409..412
                     /created_by="koroleva"
                     /label="AGTG"
     terminator      428..439
                     /created_by="hahnf"
                     /modified_by="hahnf"
                     /label="Poly-T terminator"
     overhang        440..443
                     /created_by="koroleva"
                     /label="CGCT"
     rep_origin      complement(856..1470)
                     /vntifkey=21
                     /label="rep(pMB1)"
     CDS             complement(1637..2446)
                     /vntifkey=21
                     /label="KanR"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cggtctcaag tggcaccgag
      421 tcggtgcttt tttttttttc gcttgagacc aagcttggtg taatcatggt catagctgtt
      481 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      541 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      601 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      661 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      781 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      901 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      961 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1021 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1081 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1141 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1201 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1261 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1321 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1381 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1441 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1501 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1561 gatcctttta aattaaaaat gaagttttaa atcaagccca atctgaataa tgttacaacc
     1621 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     1681 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     1741 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     1801 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1861 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     1921 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1981 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     2041 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     2101 cacctgaatc aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg
     2161 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     2221 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     2281 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg
     2341 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     2401 tggaatttaa tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat
     2461 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     2521 tgtaacatca gagattttga gacacgggcc agagctgca
//
