LOCUS       pFH15                   5647 bp    DNA     circular UNA 10-APR-2019
DEFINITION  Level0 StCas9, Arabidopsis codon optimized.
ACCESSION   urn.local...12h-abnbxnj
VERSION     urn.local...12h-abnbxnj
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             215..1225
                     /label="Sm/Sp no DraIII"
     rep_origin      1385..1999
                     /modified_by="hahnf"
                     /label="rep (pMB1)"
     rep_origin      1984
                     /label="ORI"
     misc_feature    2225..2230
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     ligation        2232..2235
                     /label="Ligation"
     CDS             2233..5628
                     /created_by="hahnf"
                     /label="StCas9"
     ligation        3303..3306
                     /label="Ligation"
     sig_peptide     5608..5628
                     /created_by="hahnf"
                     /modified_by="hahnf"
                     /label="SV40 NLS"
     Stop_codon      5629..5631
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="hahnf"
                     /label="Stop"
     ligation        5632..5635
                     /label="Ligation"
     misc_feature    complement(5637..5642)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgtctga tctcgtgctc ggactcgata tcggaatcgg atctgttgga
     2281 gtgggaatcc tcaacaaggt taccggtgag atcatccaca agaactctag gatcttccct
     2341 gctgctcagg ctgagaacaa cctcgtgaga agaactaaca ggcagggtag aaggctcgct
     2401 agaagaaaga agcacagaag ggttaggctc aacagactct tcgaggaatc tggactcatc
     2461 accgatttca ccaagatctc tatcaacctc aacccttacc agctcagggt gaagggactt
     2521 accgatgagc tttctaacga ggaactcttc attgctctca agaacatggt gaagcacagg
     2581 ggtatcagtt acctcgatga tgcttctgat gatggaaact cttctgtggg agattacgct
     2641 cagatcgtga aagagaactc taagcagctc gagactaaga cccctggaca aatccagctt
     2701 gagagatacc agacttacgg acagcttagg ggagatttca ccgttgagaa ggatggaaag
     2761 aaacacaggc tcatcaacgt gttccctacc tctgcttaca gatctgaggc tctcagaatc
     2821 ctccagaccc agcaagaatt caaccctcag atcaccgatg agttcatcaa cagatacctc
     2881 gagatcctca ctggtaagag gaagtactat cacggacctg gaaacgagaa gtctaggacc
     2941 gattacggaa ggtacagaac ctctggtgag actctcgata acatcttcgg tatcctcatc
     3001 ggaaagtgca cattctaccc agatgaattc agggctgcta aggcttctta caccgctcaa
     3061 gagttcaacc tcctcaacga tctcaacaac ctcactgtgc ctaccgagac aaagaagctc
     3121 tctaaagagc agaagaacca gatcatcaac tacgtgaaga acgagaaggc tatgggacct
     3181 gctaagcttt tcaagtatat cgctaagctc ctctcttgtg atgtggctga tatcaaggga
     3241 tacaggatcg ataagtctgg aaaggctgag atccacacct tcgaggctta cagaaagatg
     3301 aaaaccctcg aaaccttgga tatcgagcag atggataggg aaaccctcga taagctcgct
     3361 tacgtgctca ctctcaacac tgagagagag ggaatccaag aggctctcga acatgagttc
     3421 gctgatggat ctttctcaca aaagcaagtg gatgagcttg tgcagttcag aaaggctaac
     3481 tcttctatct tcggaaaggg atggcacaac ttctctgtga agcttatgat ggaacttatc
     3541 cctgagcttt acgagacttc tgaggaacag atgaccatcc tcaccaggct cggaaagcaa
     3601 aagaccacct cttcatctaa caagaccaag tatattgatg agaagctcct caccgaagag
     3661 atctacaacc ctgtggttgc taagtctgtg aggcaggcta tcaagatcgt taacgctgct
     3721 atcaaagaat acggtgattt tgataacatc gtgatcgaga tggcaaggga aacaaacgag
     3781 gatgatgaga aaaaggctat ccaaaagatc cagaaagcta acaaggatga gaaggatgct
     3841 gctatgctca aggctgctaa ccagtacaat ggtaaggctg agcttcctca ctctgtgttc
     3901 catggtcata agcagcttgc taccaagatc agactctggc atcaacaggg tgagagatgc
     3961 ctctacactg gaaagaccat ctctatccat gatctcatca acaactctaa ccagttcgaa
     4021 gtggatcaca tcctcccact ctcaatcacc ttcgatgatt ctctcgcaaa caaggtgctc
     4081 gtgtacgcta ccgctaatca agagaaggga cagaggactc cataccaggc actcgattct
     4141 atggatgatg catggtcatt cagggaactc aaggctttcg tgagagagtc taagaccctc
     4201 agtaacaaga aaaaagagta cctcctcact gaggaagata tctctaagtt cgatgtgagg
     4261 aagaagttca ttgagaggaa cctcgtggat accaggtacg cttctagagt tgtgctcaac
     4321 gctctccaag agcatttcag ggctcacaag atcgatacaa aggtgtcagt ggtgagggga
     4381 cagttcactt ctcagcttag aaggcactgg ggaatcgaaa agaccaggga tacttaccat
     4441 caccacgctg tggatgctct tattatcgct gcttcatctc agctcaacct ctggaagaag
     4501 cagaaaaaca ccctcgtgtc ttactctgag gatcagctcc tcgatattga gactggtgag
     4561 cttatctcag atgatgagta caaagagtca gttttcaagg caccatacca gcatttcgtt
     4621 gataccctca agtctaaaga gttcgaggat tctatcctct tcagttacca agtggattct
     4681 aagttcaata gaaagatctc tgatgctacc atctacgcta ccaggcaagc taaggtggga
     4741 aaggataagg ctgatgagac atacgtgctc ggtaagatca aggatatcta cacccaggat
     4801 ggatacgatg ctttcatgaa gatctataag aaggataagt ctaagttcct catgtacagg
     4861 cacgatccac agacattcga gaaggtgatc gagcctatcc tcgagaacta ccctaacaag
     4921 cagatcaacg ataagggaaa agaagtgcct tgcaacccat tcctcaagta caaagaagaa
     4981 cacggttaca tcagaaagta ctctaagaag ggaaacggac ctgagatcaa gtctctcaag
     5041 tactacgatt ctaagctcgg aaaccacatc gatatcaccc ctaagaattc taacaacaag
     5101 gtggtgctcc agtctgtgtc tccttggagg gctgatgtgt acttcaacaa gactaccgga
     5161 aagtacgaga tccttggact caagtacgct gatctccagt tcgataaggg tactggaacc
     5221 tacaagatct cacaagaaaa gtacaacgat atcaaaaaga aagagggtgt tgattctgat
     5281 tcagagttca agttcaccct ctacaagaac gatttgctcc tcgtgaagga tactgagaca
     5341 aaagagcagc agcttttcag attcctttct aggaccatgc ctaagcagaa gcactacgtt
     5401 gagcttaagc cttacgataa gcaaaagttc gagggtggtg aggcactcat caaggttctc
     5461 ggaaacgttg caaactctgg acagtgcaag aagggactcg gaaagtctaa catctcaatc
     5521 tacaaggtga ggaccgatgt gcttggaaac cagcacatca tcaagaatga gggtgataag
     5581 cctaagctcg atttctcaag ggctgatcct aagaagaaga ggaaggtttg agctttgaga
     5641 ccacgaa
//
