LOCUS       pFH79                   7354 bp    DNA     circular UNA 10-APR-2019
DEFINITION  Level0 A3A-PBE base changer, wheat codon optimized.
ACCESSION   urn.local...13w-abnbxnt
VERSION     urn.local...13w-abnbxnt
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             215..1225
                     /label="Sm/Sp no DraIII"
     rep_origin      1385..1999
                     /modified_by="hahnf"
                     /label="rep (pMB1)"
     rep_origin      1984
                     /label="ORI"
     misc_feature    2225..2230
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     ligation        2232..2235
                     /label="Ligation"
     CDS             2233..2829
                     /created_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="human APOBEC3A"
     CDS             2233..7335
                     /created_by="hahnf"
                     /label="A3A-PBE"
     Linker          2830..2877
                     /created_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="XTEN Linker"
     CDS             2884..6984
                     /created_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="nCas9 (D10A)"
     ligation        5056..5059
                     /label="Ligation"
     sig_peptide     6985..7032
                     /created_by="hahnf"
                     /modified_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="Nucleoplasmin NLS"
     CDS             7033..7302
                     /created_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="UGI"
     sig_peptide     7315..7335
                     /created_by="hahnf"
                     /Transferred_From="A3A-PBE"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 NLS"
     Stop_codon      7336..7338
                     /created_by="hahnf"
                     /modified_by="hahnf"
                     /label="STOP"
     ligation        7339..7342
                     /label="Ligation"
     misc_feature    complement(7344..7349)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggaagc gagcccagcc tcgggcccga ggcacctgat ggacccgcac
     2281 attttcacct ccaacttcaa caacgggatc ggcaggcaca agacgtacct gtgctatgag
     2341 gttgagcgtc ttgacaatgg gaccagcgtc aagatggatc agcacagagg gttcttgcac
     2401 aaccaagcca agaatctcct ctgcgggttt tacggccgcc acgcggagct ccgtttcttg
     2461 gatctcgtcc catcactgca gctcgaccca gcccagatct atcgcgttac atggttcatc
     2521 tcgtggagcc cctgcttctc ctggggttgc gcaggtgaag tcagagcctt cctccaggaa
     2581 aatacccacg tcaggctgcg cattttcgcc gcgagaatct atgattacga tcccctctat
     2641 aaggaggccc tccagatgct ccgcgatgcc ggtgcgcagg tcagcataat gacctatgac
     2701 gagtttaagc actgctggga caccttcgtg gaccatcagg gctgtccttt ccaaccctgg
     2761 gatggactcg atgagcattc tcaggccttg agcggcaggc tgagagccat cttgcaaaat
     2821 caaggtaatt ctggctctga aacaccaggt acttctgagt ccgcaactcc tgagtcattg
     2881 aaggacaaga aatactccat tgggttggcc ataggcacca actccgttgg ctgggcagtg
     2941 atcaccgacg aatacaaggt cccgagcaaa aagtttaagg tgctgggcaa tactgatcgt
     3001 cactcaatca agaagaacct gattggagcc ctgcttttcg actctgggga aacggctgag
     3061 gcaacgcgct tgaagaggac cgcgcgcagg cggtataccc gtaggaagaa tcgtatctgc
     3121 taccttcagg agatcttttc taatgaaatg gctaaggttg atgatagctt cttccaccgc
     3181 ctggaggaat cttttctcgt cgaggaggac aagaaacacg agcgccaccc aatctttgga
     3241 aacatcgtcg acgaggttgc ctatcatgaa aaatacccaa ctatatatca tctccgtaag
     3301 aagctcgtcg acagcacaga caaagcagac ttgaggctga tatacttggc attggctcac
     3361 atgataaaat tccgtggcca ctttcttatc gagggagatc tgaatccgga caacagcgac
     3421 gttgataaac tctttatcca gctggttcag acctacaatc aattgttcga ggagaacccg
     3481 atcaatgcgt ctggcgtcga cgcaaaggcc atcctgtccg cgcggctcag caaaagccgc
     3541 agattggaga atctgatcgc acagcttccg ggtgagaaga aaaacggcct cttcggcaac
     3601 ttgatcgccc tctctctcgg ccttacccct aacttcaagt cgaacttcga cctcgccgag
     3661 gacgccaagc ttcagctctc aaaggacacc tacgacgacg acctcgataa ccttctcgca
     3721 cagatcggcg atcaatacgc cgatctcttc ctcgcggcca agaacctgag cgatgcgatt
     3781 ctgttgtccg atatcctcag agtcaacaca gagatcacca aggctcccct ctccgcgtca
     3841 atgattaagc gttatgatga gcaccatcag gatctgacac tgctcaaggc tctcgtgcgt
     3901 cagcagctcc ccgagaagta taaggagatc ttcttcgacc aaagcaagaa tggctacgcc
     3961 gggtatattg acggcggcgc gtcccaagaa gaattttata agtttatcaa gccaatcctg
     4021 gaaaagatgg acggaacgga agaactgctg gtgaaattga acagggagga cctcctcagg
     4081 aaacaacgca catttgataa cgggagcatc ccacatcaaa tacatctggg tgagcttcac
     4141 gccatcctgc gccggcagga ggacttctac cccttcctga aggacaaccg ggagaagatc
     4201 gaaaagatac tcaccttcag aatcccctac tacgtcggcc cactcgccag gggcaactct
     4261 aggttcgcct ggatgactag gaagtcagaa gaaaccatca ctccctggaa cttcgaggag
     4321 gtggtggata aaggcgccag cgcccagagc ttcatcgaga ggatgaccaa tttcgataag
     4381 aatttgccaa acgaaaaggt gttgccaaag cactccctgc tttacgaata cttcacggtc
     4441 tacaacgagc tgaccaaagt gaagtacgtg acagagggaa tgaggaagcc agccttcctg
     4501 tctggggagc aaaagaaggc cattgtggac ctcttgttta agacaaatcg caaagtgacg
     4561 gtgaagcaac tcaaggagga ttactttaaa aagattgagt gtttcgactc cgtggagatc
     4621 agcggggtcg aggacaggtt taacgccagc ctggggacat atcacgacct gctgaaaatc
     4681 attaaggaca aggacttcct ggacaacgag gagaatgagg acattctcga ggatatcgtg
     4741 ctcacgctga ctctcttcga ggacagagag atgatcgagg agagactcaa gacctacgct
     4801 catctgttcg acgacaaagt catgaaacag cttaagcgta gacgttacac cggctgggga
     4861 cgcctgtccc gtaagctgat taatgggatt cgcgataagc agagcggcaa gacaatactc
     4921 gatttcttga agtccgacgg tttcgccaat aggaacttca tgcagctcat ccatgatgat
     4981 tcgctcacct ttaaggagga tattcagaag gcgcaagttt ctggccaagg cgatagcctc
     5041 cacgaacata tcgccaacct ggcgggcagc cccgcaatca aaaagggcat cctgcaaacc
     5101 gttaaggttg tggacgaact ggttaaggtc atgggcagac ataagccgga aaacatagtc
     5161 attgaaatgg ctcgcgagaa ccagacaact cagaagggcc aaaagaattc gcgggagagg
     5221 atgaagagaa ttgaggaggg aatcaaggag ctcggatcgc agattctcaa ggagcaccct
     5281 gtggagaata ctcaacttca gaacgaaaaa ctgtacttgt actacttgca gaacgggaga
     5341 gatatgtacg ttgatcagga gcttgacatc aaccgcctga gcgactacga tgtggaccac
     5401 atagtcccac agtctttcct gaaggacgac agcatcgata ataaggtctt gacccgcagc
     5461 gacaagaatc gggggaaaag cgataacgtt ccttcggagg aggtcgtcaa gaagatgaag
     5521 aattactgga ggcaattgtt gaatgccaag cttatcacgc aaagaaagtt cgacaacctt
     5581 acgaaggctg agcgcggggg tctttccgaa ttggacaagg cggggttcat aaagcgccag
     5641 ctcgtggaga ctagacaaat caccaagcac gtggcccaga ttctggactc acggatgaac
     5701 accaagtacg acgagaacga taaactcatc cgggaggtca aggttatcac cctcaagagc
     5761 aagctggtgt ccgacttcag gaaggatttt caattctata aggtccggga aattaataat
     5821 taccaccacg cccacgatgc gtaccttaac gcggtggtgg gcacggctct gatcaagaag
     5881 tacccaaagc tggagtccga gtttgtttat ggtgactata aggtgtacga tgtccgcaaa
     5941 atgattgcca agagcgagca ggaaataggc aaggcgacag ccaagtactt cttctactca
     6001 aatatcatga atttctttaa gacagaaatc acgctggcca atggcgaaat tagaaagcgt
     6061 ccgctgattg agactaacgg agagactggc gagatagttt gggacaaagg ccgcgatttc
     6121 gctaccgtca gaaaggtgct gtctatgcca caggtcaaca tcgtcaagaa aaccgaagtc
     6181 cagaccggcg gcttctctaa ggagtctatc ctgcctaaga ggaactccga taagttgatt
     6241 gctcgcaaga aggactggga cccaaagaag tacggtggat ttgactcccc gacagtcgct
     6301 tacagcgtcc ttgtcgttgc caaggttgag aaaggcaaga gcaagaagct caagagcgtg
     6361 aaggagctgt tggggatcac cattatggag aggagctcct tcgagaagaa cccgattgac
     6421 ttcttggaag caaagggcta caaagaggtt aagaaggacc tcatcatcaa gttgccaaag
     6481 tactcccttt tcgagctcga gaacgggcgc aagagaatgc tcgcctccgc aggggaactc
     6541 cagaagggca acgaattggc ccttccttca aaatacgtga attttctgta cctggcgtcc
     6601 cattacgaaa agctgaaagg gtccccggag gacaacgaac aaaagcaact gttcgtcgag
     6661 caacataagc actatcttga cgaaataatc gagcaaatct ccgagttctc taagagggtc
     6721 atccttgctg acgccaactt ggacaaggtc ctgtcagcct acaacaagca ccgggacaag
     6781 ccgatcagag agcaagctga gaatattatt cacctcttca ccttgaccaa cttgggcgcc
     6841 cccgcggcgt tcaagtactt tgacaccacg atcgatcgta agcggtacac ctccaccaaa
     6901 gaagttctcg acgccaccct tatccaccaa tcaatcactg gcctttacga aacgaggatc
     6961 gatctgagcc aattgggagg ggataaacgg cccgcagcta ccaagaaggc ggggcaggca
     7021 aaaaagaaga aaaccaggga cagcggcgga agcactaacc ttagcgacat catcgaaaag
     7081 gagacaggaa aacagctcgt tatccaagaa agcatcctta tgctccctga ggaagttgag
     7141 gaagttatcg gaaacaagcc tgagagcgac atcctggtgc acacggcata tgacgagtcc
     7201 accgacgaga acgtgatgtt gctcactagc gacgcacccg aatacaagcc gtgggctctt
     7261 gtgatccagg actccaatgg ggagaacaag attaagatgc tctctggcgg ttccccgaag
     7321 aagaaacgca aggtgtgagc tttgagacca cgaa
//
