LOCUS       pCX_mTagBFP2_mT2        6586 bp    DNA        circular     23-MAR-2023
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6586)
  TITLE     Direct Submission
  JOURNAL   Exported Mar 23, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1745..2455
                     /note="mTagBFP2"
                     /locus_tag="mTagBFP2"
     misc_feature    1943..1951
                     /note="mTagBFP2 chromophore"
                     /locus_tag="mTagBFP2 chromophore"
     misc_feature    2456..2545
                     /note="mGRASP T2A"
                     /locus_tag="mGRASP T2A"
     misc_feature    2456..2476
                     /note="Linker"
                     /locus_tag="Linker"
     misc_feature    2477..2530
                     /note="T2A"
                     /locus_tag="T2A"
     misc_feature    2531..2545
                     /note="Linker"
                     /locus_tag="Linker(1)"
     misc_feature    2546..3601
                     /note="iCre"
                     /locus_tag="iCre"
     misc_feature    2552..2572
                     /note="SV40 NLS"
                     /locus_tag="SV40 NLS"
     misc_feature    2573..3601
                     /note="cre protein sequence"
                     /locus_tag="cre"
     misc_feature    4140..4222
                     /note="lac"
                     /locus_tag="lac"
     misc_feature    4364..4439
                     /note="SV40 origin"
                     /locus_tag="SV40 origin"
     polyA_signal    4470..4544
                     /note="SV40 early region polyA"
                     /locus_tag="SV40 early region polyA"
     polyA_signal    complement(4470..4544)
                     /note="SV40 late region polyA"
                     /locus_tag="SV40 late region polyA"
     misc_feature    complement(4659..5060)
                     /note="pMB1 origin"
                     /locus_tag="pMB1 origin"
     misc_feature    5601..6461
                     /note="bla"
                     /locus_tag="bla"
     misc_feature    3614..3707
                     /note="Rabbit b-globin 3' UTR"
                     /locus_tag="Rabbit"
     misc_feature    3708..4062
                     /note="Rabbit b-globin 3' flanking region"
                     /locus_tag="Rabbit2"
     misc_feature    11..390
                     /note="hCMV enhancer"
                     /locus_tag="CMV"
     misc_feature    763..1638
                     /note="Chicken b-actin intron"
                     /locus_tag="Chicken b-actin intron"
     misc_feature    763..1687
                     /note="Ch b-actin Rb b-globin hybrid intron"
                     /locus_tag="Ch b-actin Rb b-globin hybrid intron"
     misc_feature    392..668
                     /note="Chicken b-actin promoter"
                     /locus_tag="Chicken b-actin promoter"
     misc_feature    669..762
                     /note="Chicken b-actin exon 1"
                     /locus_tag="Chicken b-actin exon 1"
     misc_feature    1647..1741
                     /note="Rabbit b-globin splice acceptor"
                     /locus_tag="Rabbit b-globin splice acceptor"
     misc_feature    1688..1741
                     /note="Rabbit b-globin sequence"
                     /locus_tag="Rabbit b-globin sequence"
ORIGIN
        1 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc
      541 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc
      601 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
      661 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg
      721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
      781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
      841 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
      901 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
      961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1021 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1081 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1141 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1201 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1261 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 cgccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
     1801 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
     1861 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     1921 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
     1981 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     2041 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     2101 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
     2161 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     2221 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa
     2281 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
     2341 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
     2401 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggcgg
     2461 ctccggcggc cgctccgagg gccgcggctc cctgctgacc tgcggcgacg tggaggagaa
     2521 ccccggcccc gcccccggct ccaccatggt gcccaagaag aagaggaaag tctccaacct
     2581 gctgactgtg caccaaaacc tgcctgccct ccctgtggat gccacctctg atgaagtcag
     2641 gaagaacctg atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat
     2701 gctcctgtct gtgtgcagat cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt
     2761 ccctgctgaa cctgaggatg tgagggacta cctcctgtac ctgcaagcca gaggcctggc
     2821 tgtgaagacc atccaacagc acctgggcca gctcaacatg ctgcacagga gatctggcct
     2881 gcctcgccct tctgactcca atgctgtgtc cctggtgatg aggagaatca gaaaggagaa
     2941 tgtggatgct ggggagagag ccaagcaggc cctggccttt gaacgcactg actttgacca
     3001 agtcagatcc ctgatggaga actctgacag atgccaggac atcaggaacc tggccttcct
     3061 gggcattgcc tacaacaccc tgctgcgcat tgccgaaatt gccagaatca gagtgaagga
     3121 catctcccgc accgatggtg ggagaatgct gatccacatt ggcaggacca agaccctggt
     3181 gtccacagct ggtgtggaga aggccctgtc cctgggggtt accaagctgg tggagagatg
     3241 gatctctgtg tctggtgtgg ctgatgaccc caacaactac ctgttctgcc gggtcagaaa
     3301 gaatggtgtg gctgcccctt ctgccacctc ccaactgtcc acccgggccc tggaagggat
     3361 ctttgaggcc acccaccgcc tgatctatgg tgccaaggat gactctgggc agagatacct
     3421 ggcctggtct ggccactctg ccagagtggg tgctgccagg gacatggcca gggctggtgt
     3481 gtccatccct gaaatcatgc aggctggtgg ctggaccaat gtgaacattg tgatgaacta
     3541 catcagaaac ctggactctg agactggggc catggtgagg ctgctcgagg atggggactg
     3601 ataaggcgcg ccggatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     3661 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3721 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     3781 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     3841 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     3901 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     3961 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4021 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     4081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4141 cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta
     4201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4261 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4441 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     4501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     4621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4741 aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag
     4801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgc 
//
