LOCUS       pSELt_S7.A              4399 bp ss-DNA     circular SYN 08-APR-2019
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      -
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gene            complement(1..1099)
     CDS             complement(180..995)
                     /note="Aminoglycoside 3'-phosphotransferase (APH)"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     -10_signal      complement(1023..1031)
                     /note
     -35_signal      complement(1044..1049)
                     /note
     misc_feature    1118..1123
     misc_feature    1191..1196
     misc_feature    1279..1284
     enhancer        1318..1332
                     /note
     promoter        1333..1395
                     /note
     misc_feature    1398..1403
     tRNA            1429..1505
     stem_loop       1429..1435
     stem_loop       1438..1454
     stem_loop       1456..1472
                     /note="NapLib Variant 2-10 Mutations: G30A - C40U A31U -
                     U39A"
     stem_loop       1473..1477
                     /note="from NapOpt"
     stem_loop       1478..1494
                     /note="NapLib Variant 3-08 Mutations: K50G - M64C G51A -
                     C63U"
     stem_loop       1495..1501
                     /note="2-10 Additional Mutation: C70U"
     terminator      1506..1584
                     /note
     misc_feature    1542..1547
     misc_feature    1585..1590
     misc_feature    1595..1600
     rep_origin      1698..2609
     misc_difference 1882
     primer_bind     1960..1988
     misc_feature    complement(2379..2409)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2602..2607
     promoter        2881..>3021
                     /note
     RBS             3010..3015
                     /note
     misc_feature    3022..3027
     CDS             3025..3258
     misc_signal     3259..3261
                     /note
     misc_feature    3262..3279
     misc_feature    3262..3269
     misc_signal     3280..3282
                     /note
     CDS             3283..4263
     misc_feature    4159..4161
     misc_feature    4265..4270
ORIGIN      
        1 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
       61 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
      121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
      181 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
      241 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
      301 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
      361 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
      421 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
      481 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
      541 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
      601 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tctcacctga atcaggatat
      661 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
      721 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
      781 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
      841 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
      901 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
      961 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     1021 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1081 agcgtcagac cccgtagaaa cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     1141 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     1201 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     1261 tcgggcagcg ttgggtcctg gccaatgcat accaaacctg cacgctagtt tcctgatgaa
     1321 catttttcca gcaattacac ctctgtcgat aattaactat tgacgaaaag ctgaaaacca
     1381 ctagaatgcg cctccgtggt accaattctt tttaagaatt gatggtatcg tggcgtagtt
     1441 cagcagggca gaacggcgat ctctaaaatc gcatgtcaga ggttcaaatc ctctcgtcgc
     1501 gaccaatttt gaaccccgct tcggcggggt tttttgtttt ctgtacattt cgtcaccctc
     1561 ccttcgcaat aaacgcccgt aatatgacca cgggtgcgca tgatcgtgca cacggtgcct
     1621 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
     1681 gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     1741 gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga
     1801 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc
     1861 ggtttttcga aggttctctg aactaccaac tctttgaacc gaggtaactg gcttggagga
     1921 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact
     1981 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg
     2041 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     2101 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     2161 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     2221 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2281 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     2341 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
     2401 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
     2461 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg
     2521 tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca
     2581 tagtaagcca gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca
     2641 ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc actggagcac
     2701 ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc
     2761 gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
     2821 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
     2881 gggccctcta gatgcatgct cgagcggcgg ccagtgtgat ggatatctgc agaattcgcc
     2941 cttcaaaaat cgatgagctg ttgacaatta atcatcgaac tagtttaatg tgtggaagcg
     3001 gccgctttca ggaggctcga gcatatgagc actatcgaag aacgcgttaa gaaaattatc
     3061 ggcgaacagc tgggcgttaa gcaggaagaa gttaccaaca atgcttcttt cgttgaagac
     3121 ctgggcgcgg attctcttga caccgttgag ctggtaatgg ctctggaaga agagtttgat
     3181 actgagattc cggacgaaga agctgagaaa atcaccaccg ttcaggctgc cattgattac
     3241 atcaacggcc accaggcgta gggcgcgccg ggcccgggct agtcacatct cgcagaactg
     3301 gttgccagtg cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agataatgtg
     3361 cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag
     3421 ctgccgccag aagagcgtcc ggcagctggt gcggttatca acgaagcgaa agagcaggtt
     3481 cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg ctgcactgaa tgcgcgtctg
     3541 gcggcggaaa cgattgatgt ctctctgcca ggtcgtcgca ttgaaaacgg cggtctgcat
     3601 ccggttaccc gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg
     3661 gcaaccgggc cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt
     3721 caccacccgg cgcgcgctga ccacgacact ttctggtttg acactacccg cctgctgcgt
     3781 acccagacct ctggcgtaca gatccgcacc atgaaagccc agcagccacc gattcgtatc
     3841 atcgcgcctg gccgtgttta tcgtaacgac tacgaccaga ctcacacgcc gatgttccat
     3901 cagatggaag gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcacgctg
     3961 cacgacttcc tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg tccttcctac
     4021 ttcccgttta ccgaaccttc tgcagaagtg gacgtcatgg gtaaaaacgg taaatggctg
     4081 gaagtgctgg gctgcgggat ggtgcatccg aacgtgttgc gtaacgttgg catcgacccg
     4141 gaagtttact ctggtttcgg cttcgggatg gggatggagc gtctgactat gttgcgttac
     4201 ggcgtcaccg acctgcgttc attcttcgaa aacgatctgc gtttcctcaa acagtttaaa
     4261 taatagatct tcgaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
     4321 cgccgtcgac catcatcatc atcatcattg agtttaaacg acgtccagct tggctgtttt
     4381 ggcggatgag agaagattt
//
