LOCUS       pFH40                   4231 bp    DNA     circular UNA 16-APR-2019
DEFINITION  Level0 Switchgrass PvUbi1 Pol II promoter.
ACCESSION   urn.local...131-abnbxnp
VERSION     urn.local...131-abnbxnp
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             215..1225
                     /label="Sm/Sp no DraIII"
     rep_origin      1385..1999
                     /vntifkey=21
                     /Transferred_From="pICSL01002_Level0_CCAT_AATG,
                     pICSL01003_Level0_TTCG_GCTT, pICSL01008_Level0_GGAG_CCAT"
                     /Transferred_Similarity="100.00%"
                     /modified_by="hahnf"
                     /label="rep (pMB1)"
     rep_origin      1984
                     /label="ORI"
     misc_feature    2225..2230
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     promoter        2236..2824
                     /created_by="User"
                     /label="PvUbi1 promoter"
     5'UTR           2825..2917
                     /created_by="User"
                     /label="PvUbi1 5'UTR"
     intron          2918..4208
                     /created_by="User"
                     /label="PvUbi1 intron1"
     misc_feature    complement(4221..4226)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagcacgt cagtgtttgg tttccactag cacgagtagc gcaatcagaa
     2281 aattttcaat gcatgaagta ctaaacgaag tttatttaga aattttttta agaaatgagt
     2341 gtaatttttt gcgacgaatt taatgacaat aattaatcga tgattgccta cagtaatgct
     2401 acagtaacca acctctaatc atgcgtcgaa tgcgtcatta gattcgtctc gcaaaatagc
     2461 acaagaatta tgaaattaat tttacaaact atttttattt aatactaata attaactgtc
     2521 aaagtttgtg ctactcgcaa gagtagcgcg aaccaaacac ggcctggagg agcacggtaa
     2581 cggcgtcgac aaactaacgg ccaccacccg ccaacgcaaa ggagacggat gagagttgac
     2641 ttcttgacgg ttctccaccc ctctgtctct ctgtcactgg gccctgggtc cccctctcga
     2701 aagttcctct ggccgaaatt gcgcggcgga gacgaggcgg gcggaaccgt cacggcagag
     2761 gattccttcc ccaccctgcc tggcccggcc atatataaac agccaccgcc cctccccgtt
     2821 ccccatcgcg tctcgtctcg tgttgttccc agaacacaac caaaatccaa atcctcctcc
     2881 tcctcccgag cctcgtcgat ccctcacccg cttcaaggta cggcgatcct cctctccctt
     2941 ctcccctcga tcgattatgc gtgttccgtt tccgtttccg atcgagcgaa tcgatggtta
     3001 ggacccatgg gggacccatg gggtgtcgtg tggtggtctg gtttgatccg cgatatttct
     3061 ccgttcgtag tgtagatctg atcgaatccc tggtgaaatc gttgatcgtg ctattcgtgt
     3121 gagggttctt aggtttggag ttgtggaggt agttctgatc ggtttgtagg tgagattttc
     3181 cccatgattt tgcttggctc gtttgtcttg gttagattag atctgcccgc attttgttcg
     3241 atatttctga tgcagatatg atgaataatt tcgtccttgt atcccgcgtc cgtatgtgta
     3301 ttaagtttgc aggtcctagt taggtttttc ctactgattt gtcttatcca ttctgtttag
     3361 cttgcaaggt ttggtaatgg tccggcatgt ttgtctctat agattagagt agaataagat
     3421 tatctcaaca agctgttggc ttatcaattt tggatctgca tgtgtttcgc atctatatct
     3481 ttgcaattaa gatggtagat ggacatatgc tcctgttgag ttgatgttgt accttttacc
     3541 tgaggtctga ggaacatgca tcctcctgct actttgtgct tatacagatc atcaagatta
     3601 tgtagctaat attcgatcag tttctagtat ctacatggta aacttgcatg cacttgctac
     3661 ttatttttga tatacttgga tgataacata tgctgctggt tgattcctac ctacatgatg
     3721 aacattttac aggccattag tgtctgtctg tatgtgttgt tcctgtttgc ttcagtctat
     3781 ttctgtttca ttcctagttt attggttctc tgctagatac ttaccctgct gggcttagtt
     3841 atcatcttat ctcgaatgca ttttcatgtt tatagatgaa tatacactca gataggtgta
     3901 gatgtatgct actgtttctc tacgttgctg taggttttac ctgtggcaac tgcatactcc
     3961 tgttgcttcg ctagatatgt atgtgcttat atagattaag atatgtgtga tggttcttta
     4021 gtatatctga tgatcatgta tgctctttta acttcttgct acacttggta acatgctgtg
     4081 atgctgtttg ttgattctgt agcactacca atgatgacct tatctctctt tgtatatgat
     4141 gtttctgttt gtttgaggct tgtgttactg ctagttactt accctgttgc ctggctaatc
     4201 ttctgcagat cctgcaatgt gagaccacga a
//
