LOCUS 62_pAAV-#62-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP62_Rpe65_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP62(Rpe65_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTTGCTTG ATCTGTTTCT CCTTTTGATT TATCTATTAC 181 AAATGTCCAT TACAATAATA AGTGAAATGC AAAGTAGATC ATGTAATTCT TCATCAAAAC 241 CTCTTCTTAT GAAGAGCTAT TGATTTCTTT AGATGAGACA AAGTTCCACA AAACTGGACC 301 TTCCCTTATC TCTAGACCAG TCTTAGGTTT ATACTCCTGA GATACAACAC CATGACCAAA 361 AGCAACCTGG AGAGAAAGTT AAATCTTACA GCTCTAGCTC TCAGGTCACA TTTCATCACT 421 GAAGTAAATC AGGGCAGGAA CTCAAGTCAG GAATTTGAAG GCAGGAATTA AAGGAGAGAC 481 CATGAATTGG TGCTGTTTAC CAGGTTGTTC TCCATGGCTT AGTCAGCCTG CTTTCTTATA 541 GCATCCAAGA CCAACTACCT ACTCATGGGT GGCCTGCCCA CAATGGGCTG TGCCCACCCA 601 CTTCAATGGC TAATTAATAA AACGTCCTAC AGACTTGCCT ACAGACCAGT CTAATAGAGG 661 TGTTTTTATC TATTATGGTT CCCTCTTCTC AGATAACTCT TGCTTATATC AAGTTGAAAA 721 ACAAGTTTCA CCTCTTTCTA GTTCATTGTA TCACATTCAC ATCTAAACTT TATAAGCTCT 781 CAAGCTTTGA CTAGCTTTTA TATAGTCTTT CTAGTTCAGA TTGCTATATT TTTCTTTTAG 841 GACTTCCTAA ACCAATGGAG ACATCCCTTC TAAGTACAAC ATCATTTTAT AGAAATTGGA 901 CCCTCAGTTG CAAATGGTGA AACCAACTTT AACTAAGTAA AAAGTGCACA AATAGTTTAA 961 ATACTAGGTA GCTAACATAA TTTCTGGAAT AGTTAAAAGA TAGAGCTTTA GGAATTAACT 1021 GTACAGTTAT TTCCTAAGTT CCTCTACACA GTGTCTTAAG TGGTCTCAGG ACTATCATGA 1081 ATGAGCAGAA TCTCCAATGA GGCCAGGCAC TGGGTGCTAT CACCACCTTC TCCTTTTGCC 1141 TATACTTGTG TCTGTGCCCC TGCTGCAAGT AAGCTGGGAA AGTTCCTCAT ATTTTTCTGA 1201 GTTCTTGGAT TGATTGCTTT ACACAGCTTT GCTGTATAAG GAAAAGTTGT TATAACCATT 1261 TAAATTTAAA TTTATCCCTT AGTTTCCTGA AAGTGATATC ATTGCTCTCT CTTGTCTATT 1321 TTCCTAGGAC TTCTGTAGAC AGCACATGTC TATCCTTAAT AATAGCAGTA GCTTAATATG 1381 AGTGTTGACA TGTGAATAAA ATGGCATTAT TTAGTCTCTG AGTGCAGAAC AAAAGCAATG 1441 GTGAAGACAG TGATGGACAA CAGGCAAGCA GTGGTGATAA GCAAAAACAT GTAGTGTTTC 1501 CTCTTTAATA AGTTCTCAGC TAAAGTTCTC AGCCTTGTTG AAAGGACCTG GATACTGAAC 1561 TGTGCCGAAG AAGGATAGCA GGGTTAAAAC ATGCAAAGAC AGCACCTCAT ATACCTCTAA 1621 TGTTGTTAAC AATAGCTAAC TTTTATCAAA CAGTGTCCTG TCACCATGAC AGTTACAACA 1681 TAATGATAAT GACTGTACTT TCTCTAACCA GGTCTAGATC ACTTATAATA AATATATCTT 1741 TTAGTAATTG AGTAAATGAA TTACAGTGAG GATAACAGCA AAGAAATGGT GGACAGATGT 1801 TTACACCAAG AAAGTATGAT GACTGAGGTC AGCTCAGGAC TGCATGGCAG GCCCACATGG 1861 CTCTTTTTTA TCCAACTCAC TACTCCCTCT CCCTTGAAAG GATCCAAGTC TGGAAAATAG 1921 CCAAAACACT GTTATGTAAA CACCAAGTCC AAATAATGTG CAAGCATCTA AAGTATTGAA 1981 AGCCACTTTT GTTACCTTCC ATCAGCTGAG GGGTGGAGAG GGTTCCCAGA GCCGCAGGCT 2041 CCTCCAATAA GGATTAGATT GCATACAAAA AAGCCCTGGC TAAGAACTTG CTTCCTCATC 2101 CTACAGCTGG TACCAGAACT CTCTCTAATC TTCACTGGAA GAAAGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT //