LOCUS       64_pAAV-#64-Chr2d-EGFP      6979 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(1418..6979,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Insertion       140..1417
                     /origin="synP64_mGluR1  (1'284bp linear)
                     Fragment: from MluI cut at 1^2 to BamHI cut at 1279^1280,
                     1'278bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
                     /label=Insert
     Restriction_sit join(<140..>143,<144..144,139^140)
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Promoter        145..1416
                     /label="synP64(mGluR1 )"
     Restriction_sit join(1417..>1417,complement(1418..>1421),1417^1418,complement(1417^1418))
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1273^1274"
                     /note="cut_0_on_negative_strand: 1277^1278"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             1429..2358
                     /vntifkey="4"
                     /label=Chr2d
     CDS             2359..3081
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   3096..3704
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3724..4202
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   4242..4382
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 4474..4780
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   5299..6156
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 6307..6974
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTtcactc tagtgttttc atgtgacctc ttgttctcaa
      181 tttagatact tcttgacctg gaaaaggtgg gtgctgcttg ccactctaca tttggctact
      241 cctgttttag gatgtgcatg ttccttcctc cccttgtctc ccgccatgaa atctcttccc
      301 atcctctttg caacctgaca acagagcctt gtccctaggt agatatacac atacattgtt
      361 tttctcattg tgttccttat ttttagagag agaaaaaaaa tctgagtctg taagataaat
      421 gcaaaggata acaatcccca aaacagccta ggaacccctc attcctctgg caagacacta
      481 ctgatgatct gtatgcttgg tagcctcttt aagtcctctc agagatagta tagtagttga
      541 caaatgccag ctcctatatt ctctagcttc tccttttcct ctatcaaatg actttttaaa
      601 taaattagat attagacaaa ccttgggtga cctgcttttt cttttctttt cctttttttt
      661 ttttttactt ctccccccgc cccccctccc ccccgttttt tttcccttgc ggtataatag
      721 tgttgaagaa agagggcact agtgtacagc ccagatggca gccttgcacc gtctgatttg
      781 gagctgaggt gtctgcgaac cgagggcggc tgcagtcctc tgacctgaga ccaatagctc
      841 tgtctacccg gactcaggta ggtcttgaag atgaaagggt gtgtgtgtgt gtgtgtgtgt
      901 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtacgcgcgc gcgcgcgcga gtgtacgaat
      961 cctagctctg agcgctgtcc agggttctga catttgagtg gctcaggcgg ttcaagcccc
     1021 aacacaggga ggctaccagg cgttgcctta gtaactaata tggcttttcc acaagctgtc
     1081 gcatttacat tttccaccat cagcgtccag cttatggcca ctaacgcgcc gctcattgca
     1141 cacctgattc acacaccttc gggcaccagt gaaaaaaccg cgacttgatt ttctggaaga
     1201 acacccctag ggtttgggag cggtcgtgga ggaccagcag gaagaagcgg aggggagagg
     1261 ggcggtggtg gagactgaga agctttgaac cagctgtgtt ggccaaaggc acgaagcggc
     1321 aaaagacagc ggcgggcgtc agggtggttc gcgctgggaa cctgcaggcg ggaccggcgt
     1381 gggaacgtgg cttgccggcg gtcgaaccgc gtcttcGGAT CCGCCACCAT GGATTATGGA
     1441 GGCGCCCTGA GTGCCGTTGG GCGCGAGCTG CTATTTGTAA CGAACCCAGT AGTCGTCAAT
     1501 GGCTCTGTAC TTGTGCCTGA GGACCAGTGT TACTGCGCGG GCTGGATTGA GTCGCGTGGC
     1561 ACAAACGGTG CCCAAACGGC GTCGAACGTG CTGCAATGGC TTGCTGCTGG CTTCTCCATC
     1621 CTACTGCTTA TGTTTTACGC CTACCAAACA TGGAAGTCAA CCTGCGGCTG GGAGGAGATC
     1681 TATGTGTGCG CTATCGAGAT GGTCAAGGTG ATTCTCGAGT TCTTCTTCGA GTTTAAGAAC
     1741 CCGTCCATGC TGTATCTAGC CACAGGCCAC CGCGTCCAGT GGTTGCGTTA CGCCGAGTGG
     1801 CTTCTCACCT GCCCGGTCAT TTGCATTCAC CTGTCAAACC TGACGGGCTT GTCCAACGAC
     1861 TACAGCAGGC GCACCATGGG TCTGCTTGTG TCTGATATTG GCACAATTGT GTGGGGCGCC
     1921 ACTTCCGCCA TGGCCACCGG ATACGTCAAG GTCATCTTCT TCTGCCTGGG TCTGTGTTAT
     1981 GGTGCTAACA CGTTCTTTCA CGCTGCCAAG GCCTACATCG AGGGTTACCA CACCGTGCCG
     2041 AAGGGCCGGT GTCGCCAGGT GGTGACTGGC ATGGCTTGGC TCTTCTTCGT ATCATGGGGT
     2101 ATGTTCCCCA TCCTGTTCAT CCTCGGCCCC GAGGGCTTCG GCGTCCTGAG CGTGTACGGC
     2161 TCCACCGTCG GCCACACCAT CATTGACCTG ATGTCGAAGA ACTGCTGGGG TCTGCTCGGC
     2221 CACTACCTGC GCGTGCTGAT CCACGAGCAT ATCCTCATCC ACGGCGACAT TCGCAAGACC
     2281 ACCAAATTGA ACATTGGTGG CACTGAGATT GAGGTCGAGA CGCTGGTGGA GGACGAGGCC
     2341 GAGGCTGGCG CGGTACCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
     2401 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC
     2461 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
     2521 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC
     2581 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC
     2641 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
     2701 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC
     2761 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG
     2821 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC
     2881 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
     2941 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
     3001 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
     3061 GACGAGCTGT ACAAGTGATA AGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT
     3121 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT
     3181 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC
     3241 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG
     3301 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC
     3361 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA
     3421 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT
     3481 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTA TGTTGCCACC
     3541 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT
     3601 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG
     3661 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGAGCGCT GCTCGAGAGA
     3721 TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA
     3781 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG
     3841 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA
     3901 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT
     3961 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG
     4021 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA
     4081 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC
     4141 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC
     4201 TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA
     4261 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC
     4321 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA
     4381 GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG
     4441 TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT
     4501 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC
     4561 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT
     4621 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT
     4681 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC
     4741 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC
     4801 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG
     4861 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC
     4921 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA
     4981 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG
     5041 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA
     5101 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT
     5161 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
     5221 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
     5281 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
     5341 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
     5401 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
     5461 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
     5521 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
     5581 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
     5641 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
     5701 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
     5761 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
     5821 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
     5881 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
     5941 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
     6001 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
     6061 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
     6121 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
     6181 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
     6241 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
     6301 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
     6361 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
     6421 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT
     6481 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
     6541 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
     6601 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
     6661 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
     6721 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
     6781 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
     6841 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
     6901 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
     6961 TGGCCTTTTG CTCACATGT
//
