LOCUS 71_pAAV-synP71-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6,977bp circular)Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5,701bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" Insertion 140..2145 /origin="synP71 (2,012bp linear)Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2,006bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP71 Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2001^2002" /note="cut_0_on_negative_strand: 2005^2006" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTATATGG GTATGTACGT GCAGTTTTGA AGATAGATCA 181 TTTCCAACTG AATCCCAACT AATTGAGGTG CAACTCAGAA ATAATGTGCA ATCGGTATAA 241 AATGGCCGAC CGGCCACGAT GTTCCCCTGA GGAATCACCA CCATGAGACA GGTCTAGTAC 301 AAAAAGAAGT TTATTGAGTG AAGTGAAGGG GACCTGGAAA GGGGGTAGAG GCAGAGAAGG 361 ATATAAAGAG GAGGAGGCTT GAGGGAAGTC CTGGGACTAT TGCTAGTCCC ATTGTAATTG 421 GCCATTGTAA TGACTTACCA CGTCTCCTAG CAGAGCACTT TAAATATTCT TAATTATCTT 481 CTTAAATTCC CATCCGAGTT AACATTATGA TTAAAAATAA GGAGAAGGAG GAATGAAAAA 541 AAATGCTCTT ATGCTTAAAT GGTCCGTCAT CTAACGGGGC GGGGGAGCGG GGGGGGGGGG 601 AGAGAAGCAC AAAACAGCTC CCTCATGCCT GGCAGGTGAC TATGCAAACT GTTGCTAGGA 661 GCTGTTGGGC GGAGCCTAGA GGAATCGCTG ACAAAGGGTA ATTGGGGAGG AAAGCAAGGC 721 TTTACTTTCA TAAAGCCCTG TGTTTAATTC CCATCACCCA AAGAGAAGAA TCTTTTAGGG 781 GTAGGCTGTC AAAAGCAGCA GGGTGAAGAG GATGCCATTG GAAACGACAG AGGAGGGGAG 841 GGCAAAGAGA CTGGTGGAGC TGTGTCCTGA GGTTAGGGCC AGGTCCTGAT GGGAGTAGCC 901 CAGCTACAAT ACATTGCTGC GAGGTCCTTT CCTTCGGCTC CGCCCCTCAA CTCCTTGGCT 961 CCTTGGCGGT TCCGGCCCTG AGCGTTCTTC GCTCGCAGCA AACAGCTTCA TACCGGAGCA 1021 GGAACGGCCA GAAAGACCCG GCTCGCTGAT ATTTAGATAT TTTTCTGTCT TTCCTGCAGC 1081 GGAAAAAGAG AGGAAGAAAG AGCATCTCAG GATGTTCTTC CGGGGCGTCC AGACAGTCTC 1141 CGCGAAGAAT AAAGCTGGTT GACTCCGGAA GCGTTTCCTT CCAGGCTAAG TCCATCTTCC 1201 GGCCTGGGCT GCCATTGCCG CGGAAGCCTC AAAGCCAGTT TTGCTAGTCG GTAAGTGAAA 1261 GTGACGGAGA CATGCTGGGG TTTGAGGCTG AAGGAGTGAG TACACCCCGC GGTGGCCTCC 1321 GCTCTAGCCT GGCACCGCAC CCACCTGACC AGTCGGGCCC GACTTCGGAA GGCTCGACTT 1381 GGGAACCGTG TGCCTCGTTG GAGGGAGTTG GCAGGCAGGC GGTCTAGGAT TCCAGTCAGC 1441 TCCTCTTGCT GTATGGGACC CTTTACCCAC TGGGAGAACA CAGAACCAGG AGGAAAAGAG 1501 TCCCTGGGAG AGGAGGAGTC ACGTTAGTTT GGGCTCAACG GAGCTAAAGC ACAAGTTGGG 1561 AAATGGAGCT GAAATCGTGA ATGTAATAGA TAGGCAAATG GGCGTTCCCG TGTTTTATTT 1621 GTACAATGAT GAACAATTTA ACATTAAGGA GCTGGGTGTA TGAAAAACGG ACCAAGATCA 1681 ATCAATAAAA GGTGGGTTAG GGTGAAATCC TGAAGGAGTT TGAATTCTTT TTAAGAACGA 1741 AAGGCTGTCT AGGAAGTGGG GGAGATGCTG GACGATTTAA GCATAAGAGC ATTAAAGAGT 1801 GCTTTGCTAG GAGACCTGTG GGAAGTCATT ACAATGGCCA ATTACAATGG GACTAGCAAT 1861 AGTGGTGGGA CAATTTGAAG ATTGTGTTTT CTAACTTAAG GGGTTAAGTG TACTTTTTTG 1921 GTGTCACTAC TGTACTGGTA TTTAAAAACT TACGTACCAT GATACAGAAA TGCATTTTGA 1981 GTTTCTGAGT TTATAGATGG TTATTGGGAA ACAGTTTTAC TCCTAGCACT CAAAGTTTGG 2041 GTCTCCATTA TTCCCAATAC ATGCTGGGAA GTGGGGGACT CAGTACCATA AAGCATTGCC 2101 TATCCAGTAT CAATGCTGTG CTCTGTTTTG CATAGATCGT GACCGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT //