LOCUS       95_pAAV-synP95-Chr2d-EGFP      7707 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(2146..7707,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6,977bp
                     circular)Target vector: from BamHI cut at 1415^1416 to
                     MluI cut at 139^140, 5,701bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Insertion       140..2145
                     /origin="synP95 (2,012bp linear)Fragment: from MluI cut
                     at 1^2 to BglII cut at 2007^2008, 2,006bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label=Insert
     Restriction_sit join(<140..>143,<144..144,139^140)
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Promoter        145..2144
                     /label=synP95
     Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146))
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="cut_0_on_positive_strand: 2007^2008"
                     /note="cut_0_on_negative_strand: 2011^2012"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     CDS             2157..3086
                     /vntifkey="4"
                     /label=Chr2d
     CDS             3087..3809
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   3824..4432
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    4452..4930
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   4970..5110
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 5202..5508
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   6027..6884
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 7035..7702
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTCCTGGA AGGGAGTATT CTGCTAGCAT ATACACCTCT
      181 ATTTTCTCTG CCTTATAATT GTTTATGGCA CTTAGCATCT GGCATCTCAT GTATGAACCA
      241 ATTTGGTCAG CATTGTCTTC TCTCAACACC TCTAGAAAGC TTTAGGAGAA GAAGACTGTG
      301 TGCCAAAGAA TGAATATTTT AGGAAAAAAA AATCAGGACT CTTTCCTTTC TTCCTTCCAC
      361 TAACGTTGCA ATCCTACTGT AGGAAGACTA CTATAGTGAG ACGTCAGAGG CACAAAGAAC
      421 TTATTAAATG GTCCTATAAA TCTAAACCAC AAAATTAAAA ACATTTTTTT TTCAGGGCAG
      481 GTGAGGTCAA AGGCCTTGTA GATGCTGAAT TCAGGTTAGA AGTCTTAAAA ATAAGATCAA
      541 GATTTTGAAG CTGGCACACA CAACCTCTTA GTCAAGTGCT CAGTGCAGCA GGGTATCAAG
      601 CTATCTCGTC ACTTAGCTGA CTATTGAAGA CTTTTGAGAT GCACTGAGTC TAACACAAGT
      661 GGGAGGAGGG GTGGACACTG GTGCACACCA ATTTAGTTTC CACAAACCAG TCATTAGTGC
      721 CTATGACATC TTCAGGTGCA TGCAGCTCGG GACATTTTCC TCTCGTGGGT AGTCGTTGGG
      781 GACTGCAGTG GAGACAGAGA AAGCAGGCCT AAAGAAGTGA CGTTCACAAT CTATATTTGA
      841 CTTGTTCTAT ATTTGCATTG CTGCTGGGGA AAGGTCCAGT GGAAAATGCG TGCTGTTCTA
      901 AATATATCCT CTCTATACCT GCTAGATTTG GATTGAAGCC TTAGGGGGCG GGGCACTGCT
      961 CAAGAGATCA CCTCCAGAGA AGCATTTGGA CTGGATTGTG TATCAGGTGT ACCTACTGCA
     1021 GACTGAGAAG GCTTGGGATC CAGTGGAAGG GTGACTTGGG TGTCACTCTG ATGCTATTTC
     1081 CTTCCTTATT CTGGGGCTCT GAGAGTGGCA GCTCAGAACA CTGTCATAAA CGCTGGGACA
     1141 TATCTTCCAG CTCTAAGGTA GTTGAGACTC AGCCTGCTGC GTTTGTTTGT TTGTTTTTAA
     1201 CGTCAGGTTC CCTGTTTATG AGATCATTTC GCTGGCGCTC TACTTCGGGC ATTTCAGTGT
     1261 TTTGTTTTCT TTCTATTGGG TGTATGAGGT GGGGGTGGGT GTTATTATAG CTTCCCAGGA
     1321 AAGAGAGTAT TTCATTCAGC ATGCTTAACT GAGTTAAGCG CAGGCGTTCA TAAATATTTA
     1381 TGAATGAGTG TATGCTAGGG TGAGTGACTT AACGGGTGAA TGGACGAGAT GCTTTAGAGT
     1441 CCGTTGAGGG CTGTGAGGAT CCGCTGTGGC TAATTTACCT CCGTAAGGGA GTTTACTTTC
     1501 TCTTTTTTTC TGTACAGTCT GTTCAGGTTT CCACCTCGGC TTAAGGGTGA AGGAAGCAGA
     1561 GCAAGAGCTC ACTGGTCTCT GAAACTCACC TCTGCTGGGC GGGAACGCGC CCGGGAGGTG
     1621 GGAGGGGGGA AAAGGGGCGG GCCGCTAAAG CAGTAGGGCG GGGCCACAGC TCAGAGGCGC
     1681 GGTCAGAGGT TCTAAGAAGG CGGAGCTGTA TGCCGGAGGG CGGGGCCAAG GGTGTGAGTC
     1741 AGAGGGCGGG GTAACAGGAG ATTCCGGGAG GGAGGAGCCG CAGGCCAGAC TGCGGAAGAG
     1801 TCTTAGGAGG GCGGGGCCAA AACGGCTGCC GTATGGATGA GGCCGCAAGG TAGAAGGGCG
     1861 TGGCCTCCAG GGAGGTCCCG TGGGAGGGAC TGCAAGTGCT AGCTAGTGGG GCTCGGGACC
     1921 CACGCGGGAG GGTGGGATAG CAAGAACGCT AGGAGGGCGG AGTTTCATGC GGTGGGGCGG
     1981 GGTCCTAGCA CCGAAGGGCG GGGCCTCGGG TGCGCGCGGG TTGGGCGGGC CTGGCGCGGC
     2041 TCCCGGGCAC AATGGCCTGA GCGGCCCGCA GAGCGTGAGA TCCACCCTGT TCGGGCCGCC
     2101 CGGCTCACCG AGCCCTCTCT CTCACGGCGC GCCGGCGTCG GGCCAGATCC GCCACCATGG
     2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG
     2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT
     2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT
     2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG
     2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT
     2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG
     2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT
     2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT
     2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC
     2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA
     2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT
     2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG
     2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC
     2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC
     3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG
     3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG
     3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG
     3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG
     3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT
     3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG
     3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG
     3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA
     3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT
     3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA
     3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG
     3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC
     3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
     3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC
     3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA
     3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT
     3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG
     4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG
     4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA
     4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA
     4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG
     4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG
     4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC
     4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC
     4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA
     4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC
     4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA
     4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT
     4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA
     4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT
     4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT
     4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT
     4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA
     4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA
     5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC
     5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC
     5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
     5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
     5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG
     5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
     5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT
     5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA
     5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
     5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT
     5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC
     5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC
     5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC
     5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA
     5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
     5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
     6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
     6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
     6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
     6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG
     6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
     6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA
     6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG
     6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
     6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC
     6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
     6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG
     6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA
     6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
     6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA
     6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
     6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
     6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
     7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
     7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
     7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
     7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
     7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
     7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
     7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
     7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
     7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
     7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
     7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
     7681 CCTTTTGCTG GCCTTTTGCT CACATGT
//
