LOCUS       102_pAAV-synP102-Chr2d-EGFP      7708 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(2147..7708,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6,977bp
                     circular)Target vector: from BamHI cut at 1415^1416 to
                     MluI cut at 139^140, 5,701bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Insertion       140..2146
                     /origin="synP102 (2,014bp linear)Fragment: from AscI cut
                     at 2^3 to BamHI cut at 2009^2010, 2,007bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label=Insert
     Restriction_sit join(<140..>143,<144..145,139^140)
                     /note="Name: AscI"
                     /note="Pattern: ggcgcgcc"
                     /note="cut_0_on_positive_strand: 2^3"
                     /note="cut_0_on_negative_strand: 6^7"
                     /note="inhibited_by: 5': 5-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=AscI
     Promoter        146..2145
                     /label=synP102
     Restriction_sit join(2146..>2146,complement(2147..>2150),2146^2147,complement(2146^2147))
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 2001^2002"
                     /note="cut_0_on_negative_strand: 2005^2006"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             2158..3087
                     /vntifkey="4"
                     /label=Chr2d
     CDS             3088..3810
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   3825..4433
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    4453..4931
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   4971..5111
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 5203..5509
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   6028..6885
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 7036..7703
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGCCTCCTC CCTGGGTGCC TGGGGTTCCC GTAGCATCAA
      181 GGCGTTCCCG CAATAAAGCT GTTGAGAAGA ATCAGACCGT GTTGCGTTTT CCTTGCCAGA
      241 CGAGGTGGGT GCGACAGGGC CATGGTACCC CCCTCACACA CTTGCTCTTG TTGCCAACTG
      301 TCTAAAGTAA GAGGGGTACC CAGTCACCGG TCCACAGAGA TGCCAGGATG TTTATCTACT
      361 AATTACCTGC TGGCTCTCAC CTCTGTTGTC TAAATTGAAA CTCCATAGTT TAGTAATGTC
      421 TCATGGTAAC CTAGGCTGGC CTTGAACATG GAATAAGCCT CCTGTTTTAG CTACACAAGT
      481 GTTGGAATGA CAGATGTGAG TAGTCACGCC CACTCAATGT CCTGTCCTGT GCTGGTTTTT
      541 TGTTGTTGTT GTTGTTGTTG TTGTTTTTTT TAGACAGGGT TTCTCCGTAT AGCCCTGGCT
      601 GTCCTGGAAC TCGCTCTGTA GACCAGGCTG GCCTTGAACT CAGAAATCCT TCTGCCTCTG
      661 TCTCCTGAGT GCTGGGATTA AAGGCGTGTA CCACCACTGC CTGGCCTCTC CTGTTTTTTG
      721 TTTGTTTTGC TTTGTTGTTG TTGCTGGTTG GTCGTTGGAA ATTTTTGAGA CTGTGTCTCA
      781 GGATATGGGA CTATCTAGGA CTATCTAGAC TATCTCTGAC TATCCTAGGA CTTGATCTGT
      841 AGATCAGGCT GTGCTTGAAC TCATAGAGAT CTGCCTGCCT CTTCCTCTCA AGTGCTGAAA
      901 TCAAAGGCTG CATGCACCAC CATGCCTTTA AAGCCCAGTT TCCAGGTTGT TTTACCTGAG
      961 GCCTGGGGAT TTGAACTTAG ACCTTTAAAC TTGAACGACA AATGTTTATT CGCTGAGCTA
     1021 TCTCCTAGGC TAAAGCCCTT GTCATATCTT ATTTAGCTCC AGACTTCAGG GTTATAGAGG
     1081 GTTTATTCTT TTATGTATAC TTTACTGGAT TGCCTTTTAG TACTTACACT AGCCATGCAG
     1141 TTATCTAAAA AAATCTGGGT TTATATATGG GTTATTTCTT TGGCTTTTGA GACAATCTTA
     1201 TGTAGCCTCT CTTTATCCTG ATCATCTTCC AATTTTTGAG TGCTGGGATT ATTACAGGTG
     1261 TGAGCCACTA CATTCAGCGG AAAAACGAAA TATTTTTTAA AAAGGAAATC TGCAATAAAA
     1321 ATGGAAACCA GAGGAGAAAG GCCGTGTGTT GGATACACTG ACTGCTCCGT TGTGGTTTGG
     1381 GAAAGGCTCT GGAGAATGAA CTTTGGTATT CTTTATTACT GCGAGTGTTA TTGCACTAGC
     1441 TCAGGTCCTT GCTGACAGGA TATTTCTCTA GCCTCCACTC TGGTCTCGGC GGTCATTGAC
     1501 TGGAAGACCA AGCCAAGACC CAGTCAAAAC TTGCGGCCCT CGGGATGGAG CGGTGGTGCT
     1561 GCTACGGCTG GTGGGAGGTG GAGGTCCCCG AAGGGTGACT AGGGGTGTAG AGGACAGGAA
     1621 GCGGTTTTGC AGCTGGAATC AGCGGAAGGA ACGTGACACA CGGTCGGCCC CACCCTCTGG
     1681 GAGGAAGAGA GGACCGGGGT CCCAGCTCCC TTCCCCTCCA AGTCGCCCCT CCAGGCTCTG
     1741 CGCGGCCGCC GGCCACGCCC CTACCAGGCC ACGCCCCCGA ACTCTGGCGC TGGCCGCGGT
     1801 GCTGAAACGC GTTTCGCAGA GCGGGCCGGG CGTGGCCTGT GAGGGCAAGC AGGAGGGGCG
     1861 GGCAAAGCGG AGGCGCAGGC CCAGACCCTG GCCGACCTAG AGGAGGCGGC AGACTCGAGC
     1921 CCATCCTGGT CAGAACGGGC ACATTCATCT GGGAAGCATC TCTTGCGAAG ACAAGGTTGA
     1981 CCTTGGCCTG CTGCACTCCC AACCTACTGG GGGGAATGTT TGCATGGCTC CCTGACCTCA
     2041 GGCTGCTCCT CACACCAGGC TGAGGACTGA CCCACGACTT CTGCTCCCTA GAGCTGTAGA
     2101 CCTGCACCCA GCCAGTGTCC CCGTCCTAGT CCAGCGGCCT TCGCCGGATC CGCCACCATG
     2161 GATTATGGAG GCGCCCTGAG TGCCGTTGGG CGCGAGCTGC TATTTGTAAC GAACCCAGTA
     2221 GTCGTCAATG GCTCTGTACT TGTGCCTGAG GACCAGTGTT ACTGCGCGGG CTGGATTGAG
     2281 TCGCGTGGCA CAAACGGTGC CCAAACGGCG TCGAACGTGC TGCAATGGCT TGCTGCTGGC
     2341 TTCTCCATCC TACTGCTTAT GTTTTACGCC TACCAAACAT GGAAGTCAAC CTGCGGCTGG
     2401 GAGGAGATCT ATGTGTGCGC TATCGAGATG GTCAAGGTGA TTCTCGAGTT CTTCTTCGAG
     2461 TTTAAGAACC CGTCCATGCT GTATCTAGCC ACAGGCCACC GCGTCCAGTG GTTGCGTTAC
     2521 GCCGAGTGGC TTCTCACCTG CCCGGTCATT TGCATTCACC TGTCAAACCT GACGGGCTTG
     2581 TCCAACGACT ACAGCAGGCG CACCATGGGT CTGCTTGTGT CTGATATTGG CACAATTGTG
     2641 TGGGGCGCCA CTTCCGCCAT GGCCACCGGA TACGTCAAGG TCATCTTCTT CTGCCTGGGT
     2701 CTGTGTTATG GTGCTAACAC GTTCTTTCAC GCTGCCAAGG CCTACATCGA GGGTTACCAC
     2761 ACCGTGCCGA AGGGCCGGTG TCGCCAGGTG GTGACTGGCA TGGCTTGGCT CTTCTTCGTA
     2821 TCATGGGGTA TGTTCCCCAT CCTGTTCATC CTCGGCCCCG AGGGCTTCGG CGTCCTGAGC
     2881 GTGTACGGCT CCACCGTCGG CCACACCATC ATTGACCTGA TGTCGAAGAA CTGCTGGGGT
     2941 CTGCTCGGCC ACTACCTGCG CGTGCTGATC CACGAGCATA TCCTCATCCA CGGCGACATT
     3001 CGCAAGACCA CCAAATTGAA CATTGGTGGC ACTGAGATTG AGGTCGAGAC GCTGGTGGAG
     3061 GACGAGGCCG AGGCTGGCGC GGTACCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
     3121 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
     3181 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
     3241 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
     3301 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
     3361 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
     3421 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
     3481 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
     3541 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
     3601 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
     3661 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
     3721 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
     3781 CTCGGCATGG ACGAGCTGTA CAAGTGATAA GAATTCGATA TCAAGCTTAT CGATAATCAA
     3841 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT
     3901 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT
     3961 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC
     4021 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG
     4081 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC
     4141 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC
     4201 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTAT
     4261 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA
     4321 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT
     4381 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGAGCGCTG
     4441 CTCGAGAGAT CTACGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG
     4501 AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT
     4561 CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC
     4621 AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG
     4681 TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC
     4741 AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT
     4801 TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG
     4861 TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT
     4921 TCCCTGTCCT TCTGATTTTG TAGGTAACCA CGTGCGGACC GAGCGGCCGC AGGAACCCCT
     4981 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC
     5041 AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG
     5101 CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA
     5161 CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT
     5221 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
     5281 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG
     5341 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT
     5401 TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG
     5461 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT
     5521 ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
     5581 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT
     5641 TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC
     5701 CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA
     5761 CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA
     5821 CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA
     5881 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
     5941 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
     6001 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
     6061 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA
     6121 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC
     6181 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA
     6241 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC
     6301 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
     6361 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
     6421 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
     6481 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
     6541 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
     6601 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
     6661 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
     6721 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
     6781 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
     6841 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
     6901 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
     6961 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
     7021 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
     7081 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
     7141 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
     7201 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
     7261 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
     7321 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     7381 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     7441 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     7501 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     7561 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     7621 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     7681 GCCTTTTGCT GGCCTTTTGC TCACATGT
//
