LOCUS pAAV-SynPVIII-Chr2d-EGFP(ITR_fix) 6124 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..141 /vntifkey="21" /label=L-ITR Vector join(563..6124,1..150) /origin="P05-49_pAAV-EF1a-Chr2d-EGFP(ITR fix) (6'988bp circular) Target vector: from BamHI cut at 1426^1427 to MluI cut at 150^151, 5'712bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._structure 39..49 /label=deletion Insertion 151..562 /origin="SynPVIII(synSBAC) (418bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 413^414, 412bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<151..>154,<155..155,150^151) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Misc._feature 156..561 /label=synPVIII(synSBAC) Restriction_sit join(562..>562,complement(563..>566),562^563,complement(562^563)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 407^408" /note="cut_0_on_negative_strand: 411^412" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 574..1503 /vntifkey="4" /label=Chr2d CDS 1504..2226 /vntifkey="4" /label=EGFP Misc._feature 2241..2849 /vntifkey="21" /label=WPRE PolyA_signal 2869..3347 /vntifkey="25" /label="hGH polyA" /label="hGH polyA" Misc._feature 3387..3527 /vntifkey="21" /label=R-ITR Replication_ori 3619..3925 /vntifkey="33" /label="f1 Origin" /label="f1 Origin" Misc._feature 4444..5301 /vntifkey="21" /label=AmpR Replication_ori 5452..6119 /vntifkey="33" /label="pUC ori" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC 61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA 121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATCTG CTTAAAAGCT CAGGAAGCCG 181 GTGCTGACAT TCTTGCTTGG GGGCCCAGAA TTTAAACTTA GTCACTGCCC AAGGCCCTTG 241 TTTTAGGAAA GAATGAGATT GTTGTTGCAT AACCAGCTGC GAGAACAGAT GGAGGCAGCG 301 CGGCTCACCT CGGGGGCTCC GTGCCCGCTG TGCGCCGAAG TCCAGGCTGA GGAGGAGGTC 361 AAATTTCATT ACCAAAGAGG CCGAGTTCTG GGGACGCTAA GCCCCCACAC AAAAAATCCC 421 TGGAGGCTCA TCACCAGCTG AGGCAGGGCC ATCAGAGAAT TTGATGGGTA GCCAGGCGTG 481 CTAACGCCAG CCCTTTGTCG GGTCTTTGTT CCACCCCTGG CTGCACTGGT ACAGAACCAC 541 TTAGCTGTGA TTAAAAGGAG GGGATCCGCC ACCATGGATT ATGGAGGCGC CCTGAGTGCC 601 GTTGGGCGCG AGCTGCTATT TGTAACGAAC CCAGTAGTCG TCAATGGCTC TGTACTTGTG 661 CCTGAGGACC AGTGTTACTG CGCGGGCTGG ATTGAGTCGC GTGGCACAAA CGGTGCCCAA 721 ACGGCGTCGA ACGTGCTGCA ATGGCTTGCT GCTGGCTTCT CCATCCTACT GCTTATGTTT 781 TACGCCTACC AAACATGGAA GTCAACCTGC GGCTGGGAGG AGATCTATGT GTGCGCTATC 841 GAGATGGTCA AGGTGATTCT CGAGTTCTTC TTCGAGTTTA AGAACCCGTC CATGCTGTAT 901 CTAGCCACAG GCCACCGCGT CCAGTGGTTG CGTTACGCCG AGTGGCTTCT CACCTGCCCG 961 GTCATTTGCA TTCACCTGTC AAACCTGACG GGCTTGTCCA ACGACTACAG CAGGCGCACC 1021 ATGGGTCTGC TTGTGTCTGA TATTGGCACA ATTGTGTGGG GCGCCACTTC CGCCATGGCC 1081 ACCGGATACG TCAAGGTCAT CTTCTTCTGC CTGGGTCTGT GTTATGGTGC TAACACGTTC 1141 TTTCACGCTG CCAAGGCCTA CATCGAGGGT TACCACACCG TGCCGAAGGG CCGGTGTCGC 1201 CAGGTGGTGA CTGGCATGGC TTGGCTCTTC TTCGTATCAT GGGGTATGTT CCCCATCCTG 1261 TTCATCCTCG GCCCCGAGGG CTTCGGCGTC CTGAGCGTGT ACGGCTCCAC CGTCGGCCAC 1321 ACCATCATTG ACCTGATGTC GAAGAACTGC TGGGGTCTGC TCGGCCACTA CCTGCGCGTG 1381 CTGATCCACG AGCATATCCT CATCCACGGC GACATTCGCA AGACCACCAA ATTGAACATT 1441 GGTGGCACTG AGATTGAGGT CGAGACGCTG GTGGAGGACG AGGCCGAGGC TGGCGCGGTA 1501 CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG 1561 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC 1621 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC 1681 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG 1741 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC 1801 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC 1861 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG 1921 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG 1981 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC 2041 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC 2101 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG 2161 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG 2221 TGATAAGAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA 2281 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA 2341 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA 2401 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG 2461 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC 2521 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC 2581 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG 2641 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTATGTTG CCACCTGGAT TCTGCGCGGG 2701 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG 2761 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC 2821 CTTTGGGCCG CCTCCCCGCA TCGATACCGA GCGCTGCTCG AGAGATCTAC GGGTGGCATC 2881 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA 2941 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA 3001 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG 3061 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA 3121 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC 3181 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA 3241 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA 3301 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG 3361 TAACCACGTG CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC 3421 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT 3481 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG 3541 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG 3601 TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 3661 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 3721 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 3781 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG 3841 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 3901 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA 3961 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 4021 AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC 4081 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC 4141 TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC 4201 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG 4261 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 4321 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 4381 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 4441 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 4501 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 4561 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 4621 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 4681 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 4741 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 4801 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 4861 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 4921 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 4981 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 5041 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 5101 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 5161 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 5221 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 5281 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 5341 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 5401 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 5461 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 5521 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 5581 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 5641 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 5701 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 5761 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 5821 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 5881 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 5941 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 6001 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 6061 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 6121 ATGT //