LOCUS       pAAV-SynPVIII-Chr2d-EGFP(ITR_fix)      6124 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..141
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(563..6124,1..150)
                     /origin="P05-49_pAAV-EF1a-Chr2d-EGFP(ITR fix) (6'988bp
                     circular)          Target vector: from BamHI cut at
                     1426^1427 to MluI cut at 150^151, 5'712bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Misc._structure 39..49
                     /label=deletion
     Insertion       151..562
                     /origin="SynPVIII(synSBAC) (418bp linear)
                     Fragment: from MluI cut at 1^2 to BamHI cut at 413^414,
                     412bp          Orientation: original          Plus strand
                     5':   no change          Plus strand 3':   no change
                     Minus strand 5':  no change          Minus strand 3':  no
                     change"
                     /label=Insert
     Restriction_sit join(<151..>154,<155..155,150^151)
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Misc._feature   156..561
                     /label=synPVIII(synSBAC)
     Restriction_sit join(562..>562,complement(563..>566),562^563,complement(562^563))
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 407^408"
                     /note="cut_0_on_negative_strand: 411^412"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             574..1503
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1504..2226
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2241..2849
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    2869..3347
                     /vntifkey="25"
                     /label="hGH polyA"
                     /label="hGH polyA"
     Misc._feature   3387..3527
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3619..3925
                     /vntifkey="33"
                     /label="f1 Origin"
                     /label="f1 Origin"
     Misc._feature   4444..5301
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5452..6119
                     /vntifkey="33"
                     /label="pUC ori"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATCTG CTTAAAAGCT CAGGAAGCCG
      181 GTGCTGACAT TCTTGCTTGG GGGCCCAGAA TTTAAACTTA GTCACTGCCC AAGGCCCTTG
      241 TTTTAGGAAA GAATGAGATT GTTGTTGCAT AACCAGCTGC GAGAACAGAT GGAGGCAGCG
      301 CGGCTCACCT CGGGGGCTCC GTGCCCGCTG TGCGCCGAAG TCCAGGCTGA GGAGGAGGTC
      361 AAATTTCATT ACCAAAGAGG CCGAGTTCTG GGGACGCTAA GCCCCCACAC AAAAAATCCC
      421 TGGAGGCTCA TCACCAGCTG AGGCAGGGCC ATCAGAGAAT TTGATGGGTA GCCAGGCGTG
      481 CTAACGCCAG CCCTTTGTCG GGTCTTTGTT CCACCCCTGG CTGCACTGGT ACAGAACCAC
      541 TTAGCTGTGA TTAAAAGGAG GGGATCCGCC ACCATGGATT ATGGAGGCGC CCTGAGTGCC
      601 GTTGGGCGCG AGCTGCTATT TGTAACGAAC CCAGTAGTCG TCAATGGCTC TGTACTTGTG
      661 CCTGAGGACC AGTGTTACTG CGCGGGCTGG ATTGAGTCGC GTGGCACAAA CGGTGCCCAA
      721 ACGGCGTCGA ACGTGCTGCA ATGGCTTGCT GCTGGCTTCT CCATCCTACT GCTTATGTTT
      781 TACGCCTACC AAACATGGAA GTCAACCTGC GGCTGGGAGG AGATCTATGT GTGCGCTATC
      841 GAGATGGTCA AGGTGATTCT CGAGTTCTTC TTCGAGTTTA AGAACCCGTC CATGCTGTAT
      901 CTAGCCACAG GCCACCGCGT CCAGTGGTTG CGTTACGCCG AGTGGCTTCT CACCTGCCCG
      961 GTCATTTGCA TTCACCTGTC AAACCTGACG GGCTTGTCCA ACGACTACAG CAGGCGCACC
     1021 ATGGGTCTGC TTGTGTCTGA TATTGGCACA ATTGTGTGGG GCGCCACTTC CGCCATGGCC
     1081 ACCGGATACG TCAAGGTCAT CTTCTTCTGC CTGGGTCTGT GTTATGGTGC TAACACGTTC
     1141 TTTCACGCTG CCAAGGCCTA CATCGAGGGT TACCACACCG TGCCGAAGGG CCGGTGTCGC
     1201 CAGGTGGTGA CTGGCATGGC TTGGCTCTTC TTCGTATCAT GGGGTATGTT CCCCATCCTG
     1261 TTCATCCTCG GCCCCGAGGG CTTCGGCGTC CTGAGCGTGT ACGGCTCCAC CGTCGGCCAC
     1321 ACCATCATTG ACCTGATGTC GAAGAACTGC TGGGGTCTGC TCGGCCACTA CCTGCGCGTG
     1381 CTGATCCACG AGCATATCCT CATCCACGGC GACATTCGCA AGACCACCAA ATTGAACATT
     1441 GGTGGCACTG AGATTGAGGT CGAGACGCTG GTGGAGGACG AGGCCGAGGC TGGCGCGGTA
     1501 CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
     1561 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
     1621 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
     1681 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG
     1741 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC
     1801 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
     1861 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG
     1921 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
     1981 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC
     2041 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
     2101 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
     2161 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG
     2221 TGATAAGAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA
     2281 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA
     2341 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA
     2401 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG
     2461 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC
     2521 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC
     2581 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG
     2641 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTATGTTG CCACCTGGAT TCTGCGCGGG
     2701 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG
     2761 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC
     2821 CTTTGGGCCG CCTCCCCGCA TCGATACCGA GCGCTGCTCG AGAGATCTAC GGGTGGCATC
     2881 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA
     2941 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA
     3001 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG
     3061 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA
     3121 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC
     3181 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA
     3241 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA
     3301 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG
     3361 TAACCACGTG CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     3421 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     3481 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG
     3541 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG
     3601 TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
     3661 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
     3721 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
     3781 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG
     3841 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
     3901 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA
     3961 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     4021 AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC
     4081 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC
     4141 TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC
     4201 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG
     4261 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC
     4321 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT
     4381 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG
     4441 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT
     4501 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT
     4561 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC
     4621 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA
     4681 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC
     4741 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA
     4801 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
     4861 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
     4921 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
     4981 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
     5041 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
     5101 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
     5161 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
     5221 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
     5281 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT
     5341 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     5401 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     5461 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     5521 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     5581 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     5641 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     5701 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     5761 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     5821 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     5881 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     5941 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     6001 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     6061 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     6121 ATGT
//
