LOCUS       12_pAAV-TF_Sox2+SV40-ChR2d-EGFP      6682 bp    DNA     circular UNA 27-APR-2012
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     Misc._feature   12..141
                     /label=5'ITR
     Promoter        150..1125
                     /vntifkey="4"
                     /label="Sox2+SV40 min"
     ORF             1132..2061
                     /label=ChR2d
     ORF             2062..2784
                     /label=EGFP
     Misc._feature   2799..3407
                     /label=WPRE
     PolyA_signal    3427..3905
                     /label="hGH polyA"
     Misc._feature   complement(3945..4074)
                     /label=3'ITR
     Misc._feature   4177..4483
                     /label="F1 origin"
     Misc._feature   5002..5859
                     /label=AmpR
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTTCAAG CCTCCTACCG CCTTTGTTAT
      181 GCAAACATAT CAAACGCCCT CCTTTGTTAT GCAAAAGGGC TGGAACGGGG CCTTTGTTAT
      241 GCAAATCGCC CTCCCCGATC CCTTTGTTAT GCAAATTTGA CGAATTCCCA CCTTTGTTAT
      301 GCAAACAAAT CTCCTACCCT CCTTTGTTAT GCAAAGTGAG AGGGGCTGCA CCTTTGTTAT
      361 GCAAAATGCG GCCCCTGAGA CCTTTGTTAT GCAAAAACCA TGTACGCTTG CCTTTGTTAT
      421 GCAAACCGCC TGTTGCTTGG CCTTTGTTAT GCAAAGCCAC GCGATTGGCG CCTTTGTTAT
      481 GCAAAGCTCG GTTATGTACA CCTTTGTTAT GCAAAGCTAC TTTAAACTTG CCTTTGTTAT
      541 GCAAATCACG ACCTGACCGT CCTTTGTTAT GCAAAAACGG TTGAAATAGT CCTTTGTTAT
      601 GCAAAATGAT ATTGAATAGT CCTTTGTTAT GCAAAAATTT AGATGCCGAC CCTTTGTTAT
      661 GCAAAGGAAT GGGCGTGCTG CCTTTGTTAT GCAAATTTTC GCTGCGACAG CCTTTGTTAT
      721 GCAAACATGC TCGCCACTCA CCTTTGTTAT GCAAAGGTCT AACAATGACC CCTTTGTTAT
      781 GCAAACTACG TGGAATAGAT CCTTTGTTAT GCAAACCCCG AGTTTTTGAA CCTTTGTTAT
      841 GCAAAATCAG TAACTTCATT CCTTTGTTAT GCAAAATGTG ACTTAACCTC CCTTTGTTAT
      901 GCAAAGCTCG AGATCTGCGA TCTGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
      961 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATCGCTGA
     1021 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
     1081 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG GATCCGCCAC CATGGATTAT
     1141 GGAGGCGCCC TGAGTGCCGT TGGGCGCGAG CTGCTATTTG TAACGAACCC AGTAGTCGTC
     1201 AATGGCTCTG TACTTGTGCC TGAGGACCAG TGTTACTGCG CGGGCTGGAT TGAGTCGCGT
     1261 GGCACAAACG GTGCCCAAAC GGCGTCGAAC GTGCTGCAAT GGCTTGCTGC TGGCTTCTCC
     1321 ATCCTACTGC TTATGTTTTA CGCCTACCAA ACATGGAAGT CAACCTGCGG CTGGGAGGAG
     1381 ATCTATGTGT GCGCTATCGA GATGGTCAAG GTGATTCTCG AGTTCTTCTT CGAGTTTAAG
     1441 AACCCGTCCA TGCTGTATCT AGCCACAGGC CACCGCGTCC AGTGGTTGCG TTACGCCGAG
     1501 TGGCTTCTCA CCTGCCCGGT CATTTGCATT CACCTGTCAA ACCTGACGGG CTTGTCCAAC
     1561 GACTACAGCA GGCGCACCAT GGGTCTGCTT GTGTCTGATA TTGGCACAAT TGTGTGGGGC
     1621 GCCACTTCCG CCATGGCCAC CGGATACGTC AAGGTCATCT TCTTCTGCCT GGGTCTGTGT
     1681 TATGGTGCTA ACACGTTCTT TCACGCTGCC AAGGCCTACA TCGAGGGTTA CCACACCGTG
     1741 CCGAAGGGCC GGTGTCGCCA GGTGGTGACT GGCATGGCTT GGCTCTTCTT CGTATCATGG
     1801 GGTATGTTCC CCATCCTGTT CATCCTCGGC CCCGAGGGCT TCGGCGTCCT GAGCGTGTAC
     1861 GGCTCCACCG TCGGCCACAC CATCATTGAC CTGATGTCGA AGAACTGCTG GGGTCTGCTC
     1921 GGCCACTACC TGCGCGTGCT GATCCACGAG CATATCCTCA TCCACGGCGA CATTCGCAAG
     1981 ACCACCAAAT TGAACATTGG TGGCACTGAG ATTGAGGTCG AGACGCTGGT GGAGGACGAG
     2041 GCCGAGGCTG GCGCGGTACC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
     2101 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
     2161 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
     2221 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC
     2281 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
     2341 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
     2401 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
     2461 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
     2521 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG
     2581 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
     2641 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
     2701 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
     2761 ATGGACGAGC TGTACAAGTG ATAAGAATTC GATATCAAGC TTATCGATAA TCAACCTCTG
     2821 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA
     2881 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT
     2941 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC
     3001 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT
     3061 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG
     3121 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC
     3181 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTATGTTGCC
     3241 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC
     3301 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT
     3361 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGAGC GCTGCTCGAG
     3421 AGATCTACGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG
     3481 CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT
     3541 AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG
     3601 GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC
     3661 AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC
     3721 CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT
     3781 AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT
     3841 ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG
     3901 TCCTTCTGAT TTTGTAGGTA ACCACGTGCG GACCGAGCGG CCGCAGGAAC CCCTAGTGAT
     3961 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT
     4021 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT
     4081 GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT
     4141 ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG
     4201 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC
     4261 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC
     4321 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT
     4381 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG
     4441 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG
     4501 GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG
     4561 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG
     4621 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA
     4681 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT
     4741 GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG
     4801 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT
     4861 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT
     4921 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA
     4981 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT
     5041 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT
     5101 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG
     5161 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG
     5221 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA
     5281 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT
     5341 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC
     5401 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG
     5461 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC
     5521 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT
     5581 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA
     5641 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT
     5701 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC
     5761 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA
     5821 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC
     5881 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG
     5941 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG
     6001 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     6061 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     6121 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
     6181 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     6241 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     6301 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     6361 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     6421 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     6481 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     6541 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     6601 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     6661 TGCTGGCCTT TTGCTCACAT GT
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