LOCUS       NEWMOL       6557 bp    DNA   circular            17-APR-2012
DEFINITION  Myc+SV40 min in pAAV-EF1a-ChR2d-EGFP.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|623586541|
COMMENT     VNTDBDATE|623586541|
COMMENT     LSOWNER|
COMMENT     VNTNAME|NEWMOL|
COMMENT     VNTAUTHORNAME|jisheng.chen|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "NEWMOL" 0 6557 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 1 0 0 30 100) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AflII" "ApaI" "BamHI" "BglII" "EcoRI" "EcoRV" "HindIII" 
COMMENT         "KpnI" "MluI" "NcoI" "PstI" "SacI" "SalI" "SmaI" "SpeI" "XbaI" "XhoI" 
COMMENT         "NdeI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList #0=(COligo "1241F" "TGTAGGTTTGGCAAGCTAGC" "" 0 (CStringList) 0) 
COMMENT         #1=(COligo "1563F" "CTGGGAAATCACCATAAACG" "" 0 (CStringList) 0) 
COMMENT         #2=(COligo "3'AD" "AGATGGTGCACGATGCACAG" "" 0 (CStringList) 0) 
COMMENT         #3=(COligo "3'AOX1" "GCAAATGGCATTCTGACATCC" "" 0 (CStringList) 0) 
COMMENT         #4=(COligo "3'BD" "TAAGAGTCACTTTAAAATTTGTATC" "" 0 (CStringList) 0) 
COMMENT         #5=(COligo "5'AOX1" "GACTGGTTCCAATTGACAAGC" "" 0 (CStringList) 0) 
COMMENT         #6=(COligo "a-Factor" "TACTATTGCCAGCATTGCTGC" "" 0 (CStringList) 0) 
COMMENT         #7=(COligo "BGH" "TAGAAGGCACAGTCGAGG" "" 0 (CStringList) 0) 
COMMENT         #8=(COligo "GAL4AD" "TCACACTACAATGGATG" "" 0 (CStringList) 0) 
COMMENT         #9=(COligo "GLP2" "CTTTATGTTTTTGGCGTCTTCCA" "" 0 (CStringList) 0) 
COMMENT         #10=(COligo "H1.3F" "GACGTCAATGGGAGTTTGTT" "" 0 (CStringList) 0) 
COMMENT         #11=(COligo "M13F" "GTAAAACGACGGCCAG" "" 0 (CStringList) 0) 
COMMENT         #12=(COligo "M13F(-47)" "CGCCAGGGTTTTCCCAGTCACGAC" "" 0 (CStringList) 0) 
COMMENT         #13=(COligo "M13R" "CAGGAAACAGCTATGAC" "" 0 (CStringList) 0) 
COMMENT         #14=(COligo "M13R(-48)" "AGCGGATAACAATTTCACACAGGA" "" 0 (CStringList) 0) 
COMMENT         #15=(COligo "PBV220F" "AAGAAGGGCAGCATTCAAAG" "" 0 (CStringList) 0) 
COMMENT         #16=(COligo "PBV220R" "CTGCGTTCTGATTTAATCTG" "" 0 (CStringList) 0) 
COMMENT         #17=(COligo "pEGFP-C-3'" "AAACCTCTACAAATGTGGTATG" "" 0 (CStringList) 0) 
COMMENT         #18=(COligo "pEGFP-C-5'" "CATGGTCCTGCTGGAGTTCGTG" "" 0 (CStringList) 0) 
COMMENT         #19=(COligo "pEGFP-N-3'" "CGTCGCCGTCCAGCTCGACCAG" "" 0 (CStringList) 0) 
COMMENT         #20=(COligo "pEGFP-N-5'" "TGGGAGGTCTATATAAGCAGAG" "" 0 (CStringList) 0) 
COMMENT         #21=(COligo "pGEX-3'" "CCGGGAGCTGCATGTGTCAGAGG" "" 0 (CStringList) 0) 
COMMENT         #22=(COligo "pGEX-5'" "GGGCTGGCAAGCCACGTTTGGTG" "" 0 (CStringList) 0) 
COMMENT         #23=(COligo "pRNA-CMV-F" "GTACGGTGGGAGGTCTATAT" "" 0 (CStringList) 0) 
COMMENT         #24=(COligo "RVP3" "CTAGCAAAATAGGCTGTCCC" "" 0 (CStringList) 0) 
COMMENT         #25=(COligo "S.tag" "CGAACGCCAGCACATGGAC" "" 0 (CStringList) 0) 
COMMENT         #26=(COligo "SP6" "GATTTAGGTGACACTATAG" "" 0 (CStringList) 0) 
COMMENT         #27=(COligo "T3" "ATTAACCCTCACTAAAGGGA" "" 0 (CStringList) 0) 
COMMENT         #28=(COligo "T7 promotor" "TAATACGACTCACTATAGG" "" 0 (CStringList) 0) 
COMMENT         #29=(COligo "T7 terminator" "TGCTAGTTATTGCTCAGCGG" "" 0 (CStringList) 0) 
COMMENT         #30=(COligo "U6-5'" "TACGATACAAGGCTGTTAGAGAG" "" 0 (CStringList) 0) 
COMMENT         #31=(COligo "PRM1" "AACGCAGTCAGGCACCGTGT" 
COMMENT              "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #32=(COligo "PRM2" "ATCCGTTAGCGAGGTGCCGC" 
COMMENT              "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT              0 (CStringList "PCR_PRIMER" "ANTISENSE_PRIMER") 0) 
COMMENT         #33=(COligo "SD1234S" "GATCCCCTATGGTGCACTCT" "" 0 (CStringList) 0) 
COMMENT         #34=(COligo "SiRNA5'" "CCTCCGATCGTTGTCAGAAG" "" 0 (CStringList) 0) 
COMMENT         #35=(COligo "pBac-1-seqR:" "CAACCCTTGTTTGAAACTAA" "" 0 (CStringList) 0)) 
COMMENT        1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6557 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #36=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 996 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BglII" "agatct" 2 0 3 787 0 1255 0 3297 0 0 
COMMENT             "") #40=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2661 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2669 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2673 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1935 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 151 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1006 0 1453 0 1507 0 
COMMENT             1936 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 3959 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 737 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 3908 0 3919 0 
COMMENT             0 "") #50=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 783 0 1293 0 3291 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #54=(CFSignal (CObList) "Myc+SV40 min" 4 0 0 150 1000 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) 
COMMENT       (CObList #55=(CMSite "1241F" "TGTAGGTTTGGCAAGCTAGC" "" 0) 
COMMENT        #56=(CMSite "1563F" "CTGGGAAATCACCATAAACG" "" 0) 
COMMENT        #57=(CMSite "3'AD" "AGATGGTGCACGATGCACAG" "" 0) 
COMMENT        #58=(CMSite "3'AOX1" "GCAAATGGCATTCTGACATCC" "" 0) 
COMMENT        #59=(CMSite "3'BD" "TAAGAGTCACTTTAAAATTTGTATC" "" 0) 
COMMENT        #60=(CMSite "5'AOX1" "GACTGGTTCCAATTGACAAGC" "" 0) 
COMMENT        #61=(CMSite "a-Factor" "TACTATTGCCAGCATTGCTGC" "" 0) 
COMMENT        #62=(CMSite "BGH" "TAGAAGGCACAGTCGAGG" "" 0) 
COMMENT        #63=(CMSite "GAL4AD" "TCACACTACAATGGATG" "" 0) 
COMMENT        #64=(CMSite "GLP2" "CTTTATGTTTTTGGCGTCTTCCA" "" 0) 
COMMENT        #65=(CMSite "H1.3F" "GACGTCAATGGGAGTTTGTT" "" 0) 
COMMENT        #66=(CMSite "M13F" "GTAAAACGACGGCCAG" "" 0) 
COMMENT        #67=(CMSite "M13F(-47)" "CGCCAGGGTTTTCCCAGTCACGAC" "" 0) 
COMMENT        #68=(CMSite "M13R" "CAGGAAACAGCTATGAC" "" 0) 
COMMENT        #69=(CMSite "M13R(-48)" "AGCGGATAACAATTTCACACAGGA" "" 0) 
COMMENT        #70=(CMSite "PBV220F" "AAGAAGGGCAGCATTCAAAG" "" 0) 
COMMENT        #71=(CMSite "PBV220R" "CTGCGTTCTGATTTAATCTG" "" 0) 
COMMENT        #72=(CMSite "pEGFP-C-3'" "AAACCTCTACAAATGTGGTATG" "" 0) 
COMMENT        #73=(CMSite "pEGFP-C-5'" "CATGGTCCTGCTGGAGTTCGTG" "" 1 2590 0 100 100 
COMMENT             100) 
COMMENT        #74=(CMSite "pEGFP-N-3'" "CGTCGCCGTCCAGCTCGACCAG" "" 1 1982 1 100 100 
COMMENT             100) #75=(CMSite "pEGFP-N-5'" "TGGGAGGTCTATATAAGCAGAG" "" 0) 
COMMENT        #76=(CMSite "pGEX-3'" "CCGGGAGCTGCATGTGTCAGAGG" "" 1 4626 0 100 100 100) 
COMMENT        #77=(CMSite "pGEX-5'" "GGGCTGGCAAGCCACGTTTGGTG" "" 0) 
COMMENT        #78=(CMSite "pRNA-CMV-F" "GTACGGTGGGAGGTCTATAT" "" 0) 
COMMENT        #79=(CMSite "RVP3" "CTAGCAAAATAGGCTGTCCC" "" 0) 
COMMENT        #80=(CMSite "S.tag" "CGAACGCCAGCACATGGAC" "" 0) 
COMMENT        #81=(CMSite "SP6" "GATTTAGGTGACACTATAG" "" 0) 
COMMENT        #82=(CMSite "T3" "ATTAACCCTCACTAAAGGGA" "" 0) 
COMMENT        #83=(CMSite "T7 promotor" "TAATACGACTCACTATAGG" "" 0) 
COMMENT        #84=(CMSite "T7 terminator" "TGCTAGTTATTGCTCAGCGG" "" 0) 
COMMENT        #85=(CMSite "U6-5'" "TACGATACAAGGCTGTTAGAGAG" "" 0) 
COMMENT        #86=(CMSite "PRM1" "AACGCAGTCAGGCACCGTGT" 
COMMENT             "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT             0) 
COMMENT        #87=(CMSite "PRM2" "ATCCGTTAGCGAGGTGCCGC" 
COMMENT             "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT             0) #88=(CMSite "SD1234S" "GATCCCCTATGGTGCACTCT" "" 0) 
COMMENT        #89=(CMSite "SiRNA5'" "CCTCCGATCGTTGTCAGAAG" "" 1 5282 1 100 100 100) 
COMMENT        #90=(CMSite "pBac-1-seqR:" "CAACCCTTGTTTGAAACTAA" "" 0)) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                   4) 
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COMMENT                          "DNA 'NEWMOL'" 1) 
COMMENT                     #95=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Myc+SV40 min in pAAV-EF1a-ChR2d-EGFP" 1) 
COMMENT                     #96=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: jisheng.chen. Original author: jisheng.chen" 
COMMENT                          1) 
COMMENT                     #97=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/17/12 10:29\311\317\316\347" 1) 
COMMENT                     #98=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/17/12 10:29\311\317\316\347" 1) 
COMMENT                     #99=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6557 bp" 1) 
COMMENT                     #100=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                           "storage type: Basic" 1) 
COMMENT                     #101=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                           "form: Circular" 1))) "General Description") 
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COMMENT                            "Original Source Database: GenBank" 1) 
COMMENT                      #104=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "Modification Date in the Original DB: 17-APR-2012" 
COMMENT                            1))) "Standard Fields") 
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COMMENT                    "Annotations") 
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COMMENT                                            "Start: 150   End: 1000" 1))) 
COMMENT                                    "Myc+SV40 min"))) "CDS (1 total)"))) 
COMMENT                    "Feature Map") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28942168 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AflII: 0 sites") 1 5 "CTTAAG" 
COMMENT                            "GAATTC") 
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COMMENT                             (CGroupPar (CParagraph 73430640 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ApaI: 0 sites") 5 1 "GGGCCC" 
COMMENT                            "CCCGGG") 
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COMMENT                                            " N1: 996 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                                            " N1: 787 " 1) 
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COMMENT                                            " N2: 1255 " 1) 
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COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                            3 3 "GATATC" "CTATAG") 
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COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                            5 "ACGCGT" "TGCGCA") 
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COMMENT                                            " N3: 1507 " 1) 
COMMENT                                      #146=(CLinePar 
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COMMENT                                            " N4: 1936 " 1))))) "NcoI: 4 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            5 "GTCGAC" "CAGCTG") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            "TGATCA") 
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COMMENT                            "AGATCT") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 73431600 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #165=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #52# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #166=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #52# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 783 " 1) 
COMMENT                                      #167=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #52# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1293 " 1) 
COMMENT                                      #168=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #52# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3291 " 1))))) "XhoI: 3 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #169=(CFolderPar 
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COMMENT                     (CObjectList 
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COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                            1) 
COMMENT                      #171=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "is calculated as an AVERAGE similarity" 1) 
COMMENT                      #172=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73431920 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #173=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #55# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
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COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #175=(COligo "1241F" 
COMMENT                                                    "TGTAGGTTTGGCAAGCTAGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TGTAGGTTTGGCAAGCTAGC" 1)))))) 
COMMENT                            "1241F (0 sites)") 
COMMENT                      #176=(CFolderPar 
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COMMENT                             (CObjectList 
COMMENT                              #177=(CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #179=(COligo "1563F" 
COMMENT                                                    "CTGGGAAATCACCATAAACG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTGGGAAATCACCATAAACG" 1)))))) 
COMMENT                            "1563F (0 sites)") 
COMMENT                      #180=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73427096 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #181=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #57# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #182=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #183=(COligo "3'AD" 
COMMENT                                                    "AGATGGTGCACGATGCACAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " AGATGGTGCACGATGCACAG" 1)))))) 
COMMENT                            "3'AD (0 sites)") 
COMMENT                      #184=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73427256 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #185=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #58# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #186=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #187=(COligo "3'AOX1" 
COMMENT                                                    "GCAAATGGCATTCTGACATCC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GCAAATGGCATTCTGACATCC" 1)))))) 
COMMENT                            "3'AOX1 (0 sites)") 
COMMENT                      #188=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73428216 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #189=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #59# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #190=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #191=(COligo "3'BD" 
COMMENT                                                    "TAAGAGTCACTTTAAAATTTGTATC" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " TAAGAGTCACTTTAAAATTTGTATC" 1)))))) 
COMMENT                            "3'BD (0 sites)") 
COMMENT                      #192=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73430240 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #193=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #60# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #194=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #195=(COligo "5'AOX1" 
COMMENT                                                    "GACTGGTTCCAATTGACAAGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GACTGGTTCCAATTGACAAGC" 1)))))) 
COMMENT                            "5'AOX1 (0 sites)") 
COMMENT                      #196=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73432320 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #197=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #61# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #198=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #199=(COligo "a-Factor" 
COMMENT                                                    "TACTATTGCCAGCATTGCTGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TACTATTGCCAGCATTGCTGC" 1)))))) 
COMMENT                            "a-Factor (0 sites)") 
COMMENT                      #200=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73422272 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #201=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #62# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #202=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #203=(COligo "BGH" 
COMMENT                                                    "TAGAAGGCACAGTCGAGG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TAGAAGGCACAGTCGAGG" 1)))))) 
COMMENT                            "BGH (0 sites)") 
COMMENT                      #204=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73433520 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #205=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #63# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #206=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #207=(COligo "GAL4AD" 
COMMENT                                                    "TCACACTACAATGGATG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TCACACTACAATGGATG" 1)))))) 
COMMENT                            "GAL4AD (0 sites)") 
COMMENT                      #208=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73434160 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #209=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #64# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #210=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #211=(COligo "GLP2" 
COMMENT                                                    "CTTTATGTTTTTGGCGTCTTCCA" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CTTTATGTTTTTGGCGTCTTCCA" 1)))))) 
COMMENT                            "GLP2 (0 sites)") 
COMMENT                      #212=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73434800 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #213=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #65# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #214=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #215=(COligo "H1.3F" 
COMMENT                                                    "GACGTCAATGGGAGTTTGTT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GACGTCAATGGGAGTTTGTT" 1)))))) 
COMMENT                            "H1.3F (0 sites)") 
COMMENT                      #216=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73425976 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #217=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #66# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #218=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #219=(COligo "M13F" 
COMMENT                                                    "GTAAAACGACGGCCAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GTAAAACGACGGCCAG" 1)))))) 
COMMENT                            "M13F (0 sites)") 
COMMENT                      #220=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73426136 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #221=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #67# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #222=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #223=(COligo "M13F(-47)" 
COMMENT                                                    "CGCCAGGGTTTTCCCAGTCACGAC" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " CGCCAGGGTTTTCCCAGTCACGAC" 1)))))) 
COMMENT                            "M13F(-47) (0 sites)") 
COMMENT                      #224=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73426296 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #225=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #68# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #226=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #227=(COligo "M13R" 
COMMENT                                                    "CAGGAAACAGCTATGAC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CAGGAAACAGCTATGAC" 1)))))) 
COMMENT                            "M13R (0 sites)") 
COMMENT                      #228=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73426456 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #229=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #69# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #230=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #231=(COligo "M13R(-48)" 
COMMENT                                                    "AGCGGATAACAATTTCACACAGGA" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " AGCGGATAACAATTTCACACAGGA" 1)))))) 
COMMENT                            "M13R(-48) (0 sites)") 
COMMENT                      #232=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73429416 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #233=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #90# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #234=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #235=(COligo "pBac-1-seqR:" 
COMMENT                                                    "CAACCCTTGTTTGAAACTAA" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CAACCCTTGTTTGAAACTAA" 1)))))) 
COMMENT                            "pBac-1-seqR: (0 sites)") 
COMMENT                      #236=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73426776 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #237=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #70# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #238=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #239=(COligo "PBV220F" 
COMMENT                                                    "AAGAAGGGCAGCATTCAAAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " AAGAAGGGCAGCATTCAAAG" 1)))))) 
COMMENT                            "PBV220F (0 sites)") 
COMMENT                      #240=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73427896 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #241=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #71# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #242=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #243=(COligo "PBV220R" 
COMMENT                                                    "CTGCGTTCTGATTTAATCTG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTGCGTTCTGATTTAATCTG" 1)))))) 
COMMENT                            "PBV220R (0 sites)") 
COMMENT                      #244=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73428696 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #245=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #72# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #246=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #247=(COligo "pEGFP-C-3'" 
COMMENT                                                    "AAACCTCTACAAATGTGGTATG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " AAACCTCTACAAATGTGGTATG" 1)))))) 
COMMENT                            "pEGFP-C-3' (0 sites)") 
COMMENT                      #248=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73428856 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #249=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #73# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #250=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #251=(COligo "pEGFP-C-5'" 
COMMENT                                                    "CATGGTCCTGCTGGAGTTCGTG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CATGGTCCTGCTGGAGTTCGTG" 1)) 
COMMENT                                     #252=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #73# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 2590 similarity 100.0% " 
COMMENT                                           1))))) "pEGFP-C-5' (1 site)") 
COMMENT                      #253=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73429016 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #254=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #74# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #255=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #256=(COligo "pEGFP-N-3'" 
COMMENT                                                    "CGTCGCCGTCCAGCTCGACCAG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CGTCGCCGTCCAGCTCGACCAG" 1)) 
COMMENT                                     #257=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #74# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 1982 similarity 100.0% on complementary strand" 
COMMENT                                           1))))) "pEGFP-N-3' (1 site)") 
COMMENT                      #258=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73423392 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #259=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #75# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #260=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #261=(COligo "pEGFP-N-5'" 
COMMENT                                                    "TGGGAGGTCTATATAAGCAGAG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " TGGGAGGTCTATATAAGCAGAG" 1)))))) 
COMMENT                            "pEGFP-N-5' (0 sites)") 
COMMENT                      #262=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73424512 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #263=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #76# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #264=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #265=(COligo "pGEX-3'" 
COMMENT                                                    "CCGGGAGCTGCATGTGTCAGAGG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CCGGGAGCTGCATGTGTCAGAGG" 1)) 
COMMENT                                     #266=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #76# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 4626 similarity 100.0% " 
COMMENT                                           1))))) "pGEX-3' (1 site)") 
COMMENT                      #267=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73425016 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #268=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #77# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #269=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #270=(COligo "pGEX-5'" 
COMMENT                                                    "GGGCTGGCAAGCCACGTTTGGTG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " GGGCTGGCAAGCCACGTTTGGTG" 1)))))) 
COMMENT                            "pGEX-5' (0 sites)") 
COMMENT                      #271=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73427656 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #272=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #86# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #273=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #274=(COligo "PRM1" 
COMMENT                                                    "AACGCAGTCAGGCACCGTGT" 
COMMENT                                                    "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " AACGCAGTCAGGCACCGTGT" 1)) 
COMMENT                                     #275=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                           1))))) "PRM1 (0 sites)") 
COMMENT                      #276=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73428376 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #277=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #87# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #278=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #279=(COligo "PRM2" 
COMMENT                                                    "ATCCGTTAGCGAGGTGCCGC" 
COMMENT                                                    "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " ATCCGTTAGCGAGGTGCCGC" 1)) 
COMMENT                                     #280=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                           1))))) "PRM2 (0 sites)") 
COMMENT                      #281=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73425176 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #282=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #78# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #283=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #284=(COligo "pRNA-CMV-F" 
COMMENT                                                    "GTACGGTGGGAGGTCTATAT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GTACGGTGGGAGGTCTATAT" 1)))))) 
COMMENT                            "pRNA-CMV-F (0 sites)") 
COMMENT                      #285=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73425336 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #286=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #79# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #287=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #288=(COligo "RVP3" 
COMMENT                                                    "CTAGCAAAATAGGCTGTCCC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTAGCAAAATAGGCTGTCCC" 1)))))) 
COMMENT                            "RVP3 (0 sites)") 
COMMENT                      #289=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73434400 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #290=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #80# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #291=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #292=(COligo "S.tag" 
COMMENT                                                    "CGAACGCCAGCACATGGAC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CGAACGCCAGCACATGGAC" 1)))))) 
COMMENT                            "S.tag (0 sites)") 
COMMENT                      #293=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73429176 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #294=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #88# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #295=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #296=(COligo "SD1234S" 
COMMENT                                                    "GATCCCCTATGGTGCACTCT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GATCCCCTATGGTGCACTCT" 1)))))) 
COMMENT                            "SD1234S (0 sites)") 
COMMENT                      #297=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 73430160 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #298=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #89# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #299=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #300=(COligo "SiRNA5'" 
COMMENT                                                    "CCTCCGATCGTTGTCAGAAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CCTCCGATCGTTGTCAGAAG" 1)) 
COMMENT                                     #301=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #89# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 5282 similarity 100.0% on complementary strand" 
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FEATURES             Location/Qualifiers
     CDS             150..1000
                     /vntifkey="4"
                     /label=Myc+SV40\min
BASE COUNT     1365 a      1866 c      1757 g      1569 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtacgtg ttacgtgacg cgcacgtggc 
      181 actcagtagg cggcacgcac gtggcgtgat gcacccgtca cgcacgtggc tgatagatca 
      241 tggcccgcac gtggcgtatc tgcaaccaac cgcacgtggc ccgaaaaatc ggcggcgcac 
      301 gtggccttta cggctttagt cgcacgtggc gccatcaagg tagtccgcac gtggctcgtt 
      361 tgtcgatatg cgcacgtggc ttgcattcga cccgtcgcac gtggccgctg aaaggtttag 
      421 cgcacgtggc ccctacgtcc tacaccgcac gtggcgggtg gcaaccaccc cgcacgtggc 
      481 gccctgactc ttgaacgcac gtggccaaag atgttccgct cgcacgtggc caagggcctg 
      541 gggtgcgcac gtggccgtat ggcgtcggac cgcacgtggc agagaacctt gttgacgcac 
      601 gtggccccat aggtctcagg cgcacgtggc gctgtgtaaa ttagacgcac gtggctaaat 
      661 atcggtcttg cgcacgtggc gatttgggaa tagtccgcac gtggcttaac ctcagaatgt 
      721 cgcacgtggc tactggtcga cggctcgcac gtggcgcatt acgttaccag cgcacgtggc 
      781 gctcgagatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
      841 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat 
      901 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 
      961 aggaggcttt tttggaggcc taggcttttg caaaggatcc gccaccatgg attatggagg 
     1021 cgccctgagt gccgttgggc gcgagctgct atttgtaacg aacccagtag tcgtcaatgg 
     1081 ctctgtactt gtgcctgagg accagtgtta ctgcgcgggc tggattgagt cgcgtggcac 
     1141 aaacggtgcc caaacggcgt cgaacgtgct gcaatggctt gctgctggct tctccatcct 
     1201 actgcttatg ttttacgcct accaaacatg gaagtcaacc tgcggctggg aggagatcta 
     1261 tgtgtgcgct atcgagatgg tcaaggtgat tctcgagttc ttcttcgagt ttaagaaccc 
     1321 gtccatgctg tatctagcca caggccaccg cgtccagtgg ttgcgttacg ccgagtggct 
     1381 tctcacctgc ccggtcattt gcattcacct gtcaaacctg acgggcttgt ccaacgacta 
     1441 cagcaggcgc accatgggtc tgcttgtgtc tgatattggc acaattgtgt ggggcgccac 
     1501 ttccgccatg gccaccggat acgtcaaggt catcttcttc tgcctgggtc tgtgttatgg 
     1561 tgctaacacg ttctttcacg ctgccaaggc ctacatcgag ggttaccaca ccgtgccgaa 
     1621 gggccggtgt cgccaggtgg tgactggcat ggcttggctc ttcttcgtat catggggtat 
     1681 gttccccatc ctgttcatcc tcggccccga gggcttcggc gtcctgagcg tgtacggctc 
     1741 caccgtcggc cacaccatca ttgacctgat gtcgaagaac tgctggggtc tgctcggcca 
     1801 ctacctgcgc gtgctgatcc acgagcatat cctcatccac ggcgacattc gcaagaccac 
     1861 caaattgaac attggtggca ctgagattga ggtcgagacg ctggtggagg acgaggccga 
     1921 ggctggcgcg gtacccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 
     1981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 
     2041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 
     2101 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 
     2161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 
     2221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 
     2281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 
     2341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 
     2401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 
     2461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 
     2521 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 
     2581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 
     2641 cgagctgtac aagtgataag aattcgatat caagcttatc gataatcaac ctctggatta 
     2701 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     2761 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     2821 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     2881 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     2941 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     3001 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     3061 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg 
     3121 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     3181 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     3241 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 
     3301 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 
     3361 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 
     3421 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 
     3481 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 
     3541 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 
     3601 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 
     3661 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 
     3721 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 
     3781 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 
     3841 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     3901 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     3961 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4021 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     4141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     4381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     4501 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     4561 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     4621 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     4681 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     4741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     4801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     4861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     4921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     4981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     5521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     5581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     5641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     5701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     5761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     5821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6541 gccttttgct cacatgt 
//
