LOCUS 46_pAAV-#46-Chr2d-EGFP 5937 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(376..5937,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..375 /origin="synP46 (242bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 237^238, 236bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..374 /label=synP46(TF_Sox2_1+SV40) Restriction_sit join(375..>375,complement(376..>379),375^376,complement(375^376)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 237^238" /note="cut_0_on_negative_strand: 241^242" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 387..1316 /vntifkey="4" /label=Chr2d CDS 1317..2039 /vntifkey="4" /label=EGFP Misc._feature 2054..2662 /vntifkey="21" /label=WPRE PolyA_signal 2682..3160 /vntifkey="25" /label="hGH polyA" Misc._feature 3200..3340 /vntifkey="21" /label=R-ITR Replication_ori 3432..3738 /vntifkey="33" /label="f1 Origin" Misc._feature 4257..5114 /vntifkey="21" /label=AmpR Replication_ori 5265..5932 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTGCCTTT GTTATGCAAA GCTCGAGATC TGCGATCTGC 181 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 241 CGCCCAGTTC CGCCCATTCT CCGCCCCATC GCTGACTAAT TTTTTTTATT TATGCAGAGG 301 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 361 TAGGCTTTTG CAAAGGATCC GCCACCATGG ATTATGGAGG CGCCCTGAGT GCCGTTGGGC 421 GCGAGCTGCT ATTTGTAACG AACCCAGTAG TCGTCAATGG CTCTGTACTT GTGCCTGAGG 481 ACCAGTGTTA CTGCGCGGGC TGGATTGAGT CGCGTGGCAC AAACGGTGCC CAAACGGCGT 541 CGAACGTGCT GCAATGGCTT GCTGCTGGCT TCTCCATCCT ACTGCTTATG TTTTACGCCT 601 ACCAAACATG GAAGTCAACC TGCGGCTGGG AGGAGATCTA TGTGTGCGCT ATCGAGATGG 661 TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT TTAAGAACCC GTCCATGCTG TATCTAGCCA 721 CAGGCCACCG CGTCCAGTGG TTGCGTTACG CCGAGTGGCT TCTCACCTGC CCGGTCATTT 781 GCATTCACCT GTCAAACCTG ACGGGCTTGT CCAACGACTA CAGCAGGCGC ACCATGGGTC 841 TGCTTGTGTC TGATATTGGC ACAATTGTGT GGGGCGCCAC TTCCGCCATG GCCACCGGAT 901 ACGTCAAGGT CATCTTCTTC TGCCTGGGTC TGTGTTATGG TGCTAACACG TTCTTTCACG 961 CTGCCAAGGC CTACATCGAG GGTTACCACA CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG 1021 TGACTGGCAT GGCTTGGCTC TTCTTCGTAT CATGGGGTAT GTTCCCCATC CTGTTCATCC 1081 TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG TGTACGGCTC CACCGTCGGC CACACCATCA 1141 TTGACCTGAT GTCGAAGAAC TGCTGGGGTC TGCTCGGCCA CTACCTGCGC GTGCTGATCC 1201 ACGAGCATAT CCTCATCCAC GGCGACATTC GCAAGACCAC CAAATTGAAC ATTGGTGGCA 1261 CTGAGATTGA GGTCGAGACG CTGGTGGAGG ACGAGGCCGA GGCTGGCGCG GTACCCATGG 1321 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG 1381 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA 1441 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG 1501 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC 1561 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA 1621 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA 1681 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC 1741 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA 1801 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC 1861 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC 1921 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC 1981 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTGATAAG 2041 AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 2101 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 2161 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 2221 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 2281 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 2341 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 2401 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT 2461 CATCGTCCTT TCCTTGGCTG CTCGCCTATG TTGCCACCTG GATTCTGCGC GGGACGTCCT 2521 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 2581 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 2641 CCGCCTCCCC GCATCGATAC CGAGCGCTGC TCGAGAGATC TACGGGTGGC ATCCCTGTGA 2701 CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT 2761 CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG 2821 GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG 2881 GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC 2941 CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG 3001 CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC 3061 CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT 3121 GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTGT AGGTAACCAC 3181 GTGCGGACCG AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC 3241 GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG 3301 GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT 3361 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC 3421 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 3481 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 3541 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 3601 TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC 3661 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 3721 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA 3781 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 3841 ATTTTAACAA AATATTAACG TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG 3901 ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG 3961 CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT 4021 GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC 4081 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 4141 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 4201 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 4261 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 4321 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 4381 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 4441 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 4501 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 4561 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 4621 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 4681 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 4741 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 4801 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 4861 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 4921 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 4981 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 5041 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 5101 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 5161 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 5221 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 5281 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 5341 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 5401 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 5461 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 5521 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 5581 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 5641 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 5701 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 5761 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 5821 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 5881 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 57_pAAV-#57-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="SynP57_Prph_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP57(Prph_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTGCTCAG GCTCTTGGGG ACTGGGCTCC AGCCCTCTGG 181 GATCATCATT TGCTCTAAGA ACTGGCCTGG GTGCAGCTCC AGACCAAAGG CAGCAATTGT 241 TCAGAGCCCT GAAAGCGCCA AGGCGCCAAG GCTTCTTCTA CATACTCACC TCTGACCCAC 301 CAGCCCCCCA CCCCAGCCCA GGTCTGACGA AAGGTACCTC TCTCCACTGC AACAACTGGG 361 GTGTGGCAGG CTCTGGTTTA TTCGCCTTGT TCTCCCTTCC CCAACCCCCC TTTTCTCATC 421 CCCCTAGCAA CCAAACTAGA TCCATCAAAG AGCAGGACCT GGCAGCCGAG CTGGGAGAGA 481 CTAATAGCCT GGAAGGAAGG CGGGGCCTGG AGAGGAACGG AAGCCTAGGG ATGCAAGCCA 541 GCACTGGGCG TTGGCTCTGA CCCATCTCGG AGGACACACG GAAGGTGGGG GAGTTCTCTG 601 CTCTGCAGTC TGCAGGGAGC CATCCTCCTT ATCCCAGTCA GGCATCCAGC CTAGAACCCC 661 AAGCCTTCTT CTCTTACACC CGTCTCTTTC TCAGGACCCA ACTGAGGTAG ACTCATCCTG 721 TTTGAGAGTC CCAGGGTCCC CAGTGGTAGC AGACACATGG CTCTCAGCAA ACCCAAAGGG 781 CTTCAGCATC CTTTCTCCTG CAGAGAATCC AGACGGCCTC TGTCCACTCC TGGGACTGCC 841 TGTGCTGCAT TCTGGAAGTA GTGTGTCACA CAAAGGTCAG ACACCAGCCT TTCTGCTAAC 901 TGGGGTGTGG GGGCGCTGTT AAGGGGTGTA GCTGTGTATT CCTGTCATGT CTGTGCACAC 961 ATGCATATTT GTAGCCTCTA CAAAGCTGGC TCAGTGAGTA TTGGGCAAGT TATCTGTGGA 1021 CCTGTCGGAG GACTTCTCTC TCTAACAGGC TGTAGTGGCT GGGTATCTCT CCCATCTCAT 1081 CTCCCTTTAT CTGCACCATG TCTGGGTACC TGCATCTCCT CTGCACTGGA GACTGGTGCC 1141 TACTAGTCTA TATGTCTTTC AGCCCTGGCA GCTGCTATCC CCCACCCCCC TCCCCTTCCT 1201 ACTTCAGGAA TTCCTCTGGT TCCCGTAAGG CCCGTGACTG CCCAGCAGAT GGTGTGGAGG 1261 GGGCACCAAT CCAGTAAAGG CTGAAAGTGT ACCACAGGCC CACTCAGCCC CAACAAGAGT 1321 GGGCACCTCA CAGGCCCTTT CATGGCACAG ACCCTTGGAA CCCCGACATC CTCAGCACCC 1381 TGTGAGGTGC CCACTCTTGT TGGGGTGGGT GTTACGTCCG AGTTTGGGGG CTGTGTCTTT 1441 AAGATGGAAA CATCACCATG CAACTTCTGC TGGTCCAAGG GCGGGGGTGG GGGTGGGAGA 1501 GCTGGTCAGT CCATTAGCTG CAGAGCTGGC GCCAATCACC AGCCCTTTAC CGTGCCCTGG 1561 GGAGTAGGCA GAGATAAGCT CTTCCCCAGC TCCCTCTGCC TCAGCCCTCG GTTGTGGCCA 1621 ATGATGGGGG GCAGTTGACA ACAGGTGAAA GGAGAACCCC AGTTTCAGGA GACAGGAGGA 1681 GGCACGAATT CCCTGGCTTA GGCCAGGTTA GCTCTCCCTC CACCTACCCC ACTTCTCATT 1741 GCTCAAAACT TGCCCTTTTC CTCAGGTCCT CATATTCCCT AATTTTTACC CCCTCTTCTG 1801 AGAGGGCACC CCAGGTCAAG CCATGTCCTC CCATTCTAGG CTCCAGCGTT GGATGCATGC 1861 TCTAAGGTAG ACCTTAGCCC ACCTCCATCA CATCCCGGAT CTCAGCCAGC AACAAGGGGG 1921 AATCAAGCAG GCAGGGTGCC AGCAACCAGG AGAGGGAAGG GGTGGTGTCC TCTCTCTGCA 1981 GGGTGGGGCA TCCCCCTCCC CACACAGCCC AAGGCTGAAG TCAGGCCAGT GGGAGGAGCT 2041 GTCGTGGCCC CCCACCCCCC CTCCCCGGAG ACCGCAGGGC TATAAAGCCG CCCCGCATCG 2101 GTCTGCAGCT CCTTGCCACC CGGCCTAGTT CTGCCAAGCG CTGAGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 58_pAAV-#58-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP58_Hbb-b1_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP58(Hbb-b1_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTTTTTTC AGAGGCTGCA CTCTGGTTTT CTGTCTCTCC 181 ATTTTCTGCA GGCAGTTCTC TAGTTTGCTG GTCAGCCACT TCAGCCTGTT TGCCCTTCTC 241 TCCCCTCTTC CCTTTTATCT GCACTTTTTT TGTCTGATGC TTTATTCTTT CCTGCAGCCC 301 CTTTGGGCTT CCTGTCATTG ATGGGGCAGG CTTGGCTAAT CGCCTTGCAG AATGGTGCTT 361 GGGGTATGCC TTCACTGCTC CATCTCACCT TCCTCTTGGG AATCATAGCT GTGCCGGGTG 421 AGAGATACAT CCATCGCTGG ACCTGTCAAG GAGCTGCACT GCCTAGCCTC CACCACACGA 481 AATGCCAGTA TTTTTTTTCA ACTGTGCTTT TTATTCACAA ATAACTCTAG CTTGTGTCAA 541 GTTGACAAAA CAAAATCCAA CCAGAGCTTG GGGTAAAAAG TGAAGAAAAT GATTCAAAAT 601 ATTACTATAC TTACACTTAG TACACTTAGT GTCAAGCAGA CACAGAAACT GACTGACTGA 661 CTGAATGAAT GAACAAATGA AGAAATGAAT GAATGAGTCA GTCAGTTAGT TACTGATAGA 721 ATTCCTTTCT ACCTTCTTCC TATTTGGCTC CAGGCACATT CCCACATCAA GATAAAGAGA 781 AACAGGTATA GCTACATAGT CTCTACTTTT AGACAATCGC GTCTACTAAT GTTTCAAAAC 841 ATTGAGTTCT AAATAATTTT ATACTGATTT CTATGTCTTT AGAATTGAGA TTTCTCCATT 901 TGCATCTGCA GATCCCAAAA ATTGAATAGA ATCACTACTG CACAGGGTAT GCTCAATTCA 961 AATGTACCTT ATTTTAAAAC ATTATTTAGC AATGTGTAAA AGTGAAATAA AGAGGTAAGT 1021 TCACATACAA ATAGAGAGGC ATACAAACAT ACATGCATAA TACACATACA TACACACACA 1081 GACACACAGA CACACAGATA TACAGAGATA CAGACACACA GACACACACA CACAGGTACA 1141 TATACAAATA CACACACACA TATTCACAGA TATACAGAGA TACAGACACA CTCAGAGACA 1201 TACACATGGA CATGCATACA AACACACAGA TACACACATA CACACACATG GGCAGCATGT 1261 GCTGAGGACT TGGTTCAGTA AATAAAGGTC AAGGCTGCCT GCCTTTAATT CAAAAGCATG 1321 GAAGACAGGA CAATCCCTGA AAAAGCAGGT TATCCAAGCT AACCAGATTT GTGAGCTCAG 1381 GGTTTACTTA AGAGATCCTG ACTCAACAAT AAGGTATAGA GCAATCAAGG GTGATTCTCT 1441 GAAGGCAGTT ATTGAACTCC TTGTACACTC CTCCCACACA CATGTGTTCA GAAAACTTGA 1501 TAAAACACAT ATATACAAGT AAACCACACA AACATTCACA TGAGAGAAGA AAAAAAAGAG 1561 TAAATAAAGT AAGATGCATT TTCTTATCAG GAAGTTTAGT TGACACCAGA AAGAAGTCAT 1621 ATTTGGAATC AAGATGGAGT CATCATCATG TATGCTAAAG ATGTTTTTTC CACATTCTTG 1681 AGCAATGTGG ACAGAGAAGG AGATTCATCC ATGCACTCAA ACTGAGAAAC AAAGAAAAGA 1741 AATCCTCTTC TAAGCTTTGC TTCTCAATTT CTTATTTGCA TAATGAGAAA AAAAGGAAAA 1801 TTAATTTTAA CAACCAATTC AGTAGTTGAT TGAGCAAATG TGTTGCCAAA AAGGATGCTT 1861 TAGAGACAGT GTTCTCTGCA CAGATAAGGA CAAACATTAT TCAGAGGGAG TACCCAGAGC 1921 TGAGACTCCT AAGCCAGTGA GTGGCACAGC ATCCAGGGAG AAATATGCTT GTCATCACCG 1981 AAGCCTGATT CCGTAGAGCC ACACCCTGGT AAGGGCCAAT CTGCTCACAC AGGATAGAGA 2041 GGGCAGGAGC CAGGGCAGAG CATATAAGGT GAGGTAGGAT CAGTTGCTCC TCACATTTGC 2101 TTCTGACATA GTTGTGTTGA CTCACAACCC CAGAAACAGA CATCGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 59_pAAV-#59-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP59_Mfsd2(a)_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP59(Mfsd2(a)_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTCTTAGG CTGCATTAAC AATGTGTCGT GTCCAGACTG 181 GGGGAAATAA TAGGTCTATT GTACTGGCTT GGTCAGGCCA TATCTGGAAT CTTGCGTTGC 241 TACACTGACA GACTGGAATC GGGCCAGGAG GGGAACTAGC CCAGCCTCAG CTCCTGAGTC 301 CTTGTGCGCG AAATGTTTCC TGTACAACTT CTTGTCTTAT CCCCCAGACA ACGCTATTAG 361 GTAGGTCCCT ATTTTGTACA CAAGGAGGTT AATAAAGTTG TTCAGGAACA ACTAGATAGC 421 CCGTCCATGG AGGTGCCTGG GGTTTTTCTG AATGGGCTGG CTCCTCTGGT GCTTCAGCTC 481 ACACTCACTG TGATTTAGTG GGATATGGGA GTGAAAGAGA TAGAGTTCTT ATTTTCAAGG 541 AGGGCATAAT CTGGCTCGGT TATTCAGATC ACTTCTGGAA TGCTGCCATT CAAAGGAGTG 601 AAGAATTTAG GGCTGGAAGA GACTGTACAG GACTCCATAG CTTCCCTCCT ATAGTATGGA 661 AGAGAGAGAG GGCAGTGTGA CAGTGGGGAC AACAGGCCTT CTTACAGTCT TCACTATTTC 721 CCTTGGCTTG TTTCTGGACA GTGATGTGCC AGGGCACACA AGGGTGCTGT GGCCATTGTG 781 GAGGAGTCAG ATCTCAATAG CTCTTCCCTG CAATCTGAAC TAAGAGGAGG GGAAAAAAAA 841 AAAGCTTGAC ACTGAGAAAC ACAGGTCCTG TGTGAGCGGT CTCTGAGAGT CTAAGCAGTA 901 ACTTCACTGA AGAGGGAGAG CTGGACTTGA CCCAGGCTCC TGGGGATCTG TGAAGGCTGG 961 AGTGCCATTA GCAGAGGTGG AAGGAAAGGC AGCAAAGACC GTTCTCTGCT GGCACCTATG 1021 CCTGGCCTCT GTTTAGATGA TGACGGTCCT CCCTTCTTTC AAGGACCACA TTCCTCTGCA 1081 TGCTCTTTCT CAAAGCTTTT CTTTTCTCTT CTCTCTTTTC TTTTCTTTTC TTTTCTTTTC 1141 TTTTCTTTTC TTTTCTTTTC TTTTCTTTTC TTTTCTTTTC TTTTCGACAC AGAGGGCTGC 1201 TGGCGTCTTG TATCTCCCAG GGCTTCCTGT CTGGGAGCAC AGACATAGCT CAGCCTTCTG 1261 TAATTTATGC TGAGGGGCAG AGCAGGAGAG TTATGAGTAA AGACTTTGGA ACTGGACAAA 1321 ACCTTTCTGG CCTGTCTGTG TCGCAACAGC TGTTCTTCTT GTGTCCCCCT TTCCTGGGTT 1381 GTAAAATGGG GATAATAATA GTACCACCCT CAGATGGCGT TCATAAGCAC CCAGTGAGGT 1441 CATGTTAAGC CTGTTAGAAA GCTACCCATA AACGGCTGCT GTGGCCATGG CTCCTTCTTA 1501 GAACTTGCGA GTTCCCCTGC CTGGAAGCGG TAGCCACTGG ACTTGGTAAT TTTTCTGTCC 1561 CATTGAAAAA GGCCCCTACT TTTCAAAGGG AGGAAGAGGA AATTGTCGAT CTTGAGACAC 1621 GAGGGACATT GATAATGAAA GTTACAGCCC ATGGCCTCAG GTTTGGGGGA GGGGAGAAGC 1681 CACCTGACTC CGGATTTTAA AAGGAGCTGA TGGGGATTCA GAAGTCAAGG GCCACAGGCT 1741 CTACTCAGGG AAAACTCACT CTGGGGGACC TCGGACAGGT CACTTTCAGC CTGGGACTTC 1801 AGTTTCCTCG GGCGAGTCAG GTGGGTTTCT CAGCTTTGCT TAGGTAGTTA GGAGCACGCG 1861 GTGTGGGTTG CCGGCTTTGA GAACGTGGCT GAGCAGCGAG GCTCCGCCAG CCGAGATGGG 1921 CAGGCGCGTG CAGGGATTGG CTGGAGGCGC AGGCCCGAAC CTTTAAGAGG CGCGGAGGGG 1981 ACGTGCTAGG GAGTTTTCAT CGCCGGCGTT CCTGGTTTGC TAAGTGCTAG CGAACGTAGT 2041 AGTGGCTGGG GCTGCGCCTA GGATCGGAGC CACAGGCGAG TGCCGGCCGG CAGAACACAA 2101 GAGCATCCCG GCGTCCCGGT CCCACGCGGC GTGGCCCGCG GGTCGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 60_pAAV-#60-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP60_Rdh5_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP60(Rdh5_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTAAGAGT GCAGAGATGG TGATCTGATT GTCTATGGGA 181 AGAAAGGAGG CACATACTAT GCCTTCCGCC CAGGGCGAGT GACAGGCTTG GTTGCAAATC 241 CAGAGACTGA TTCATCCAAG GGAGTCATGC AGGGTTAGGG TCATCAACCA TTACTCATCT 301 TGAGGGGAGA TTAGGGGGAA GAATGGCTGT GGTTGGGGGC TCTGGTCTCC TTTGTCTCCA 361 GACAGCTCTC TCCTCCTCTT CTCTGGTGTT CCCACACTGG CACATCCTTT GCTACATTTG 421 TGACGCTGGC CAGACTTCTT TCTGTTTACT TGCTAGACGC CACCCCACAT TCGAAGCTTA 481 GAGGGCACCT AAGCCAAATC CTGATTAGAA AGGGTTTTGT GTTGCTCCCC ACAGCTCCTA 541 TTCTCCAGAA AATAAAACAA AGGCCTAGTT TGTCTCAGCC CTTGTCAGGT GCCCTGCCCA 601 CTTTCTTGAA GCCCCCCCCC CCACCCAGCC CTTTGCCTCC TCTGCCCCCA CACATTCCAG 661 TGGGTGAGGG CACCGGATGT GGAGTCTCCT TACTGACCTA GAGCTTTGGA ATAGGGAATG 721 AAAAGGTCAT CTGCTAGTAA GAGAGGGAAC ACTGGTTATG TAAAAGGGAA AGTGAAAAAC 781 ACTGGTGGAG GATGGAGAGC TAACCTCTTA GCAGTGATAG TTACCACAGC CAGCACCTTG 841 ACTGAGCTGA GAAGTGTCAG CACTGGGCAG TGTGGGTGGG GTGGTGTTCA CTCATCTGTG 901 TGGGTGGGAG AGTTGGGTCT GAAGGTGAAA GCCTGTGATC CCAGCTCTTG GACTGTAGAG 961 GCAAGAGGAT TAGATCAAGG CCAGCTTCAT CCACATAGCG AGTTTGGGGT CATCCTGGGC 1021 TACATAAGAC CCTGTCTCAA AACGGGGCCT GGGAAGAACC AAGAAAATCT CTTGGGGTAG 1081 GCTCATTTGA CCCAGGATTC CTGAGGAGGG GGTGTGGTCT CCCATCCCAC TCCAGATCCA 1141 GGGAGGGGCC TGACTTCCTT TGCAACTTCT TGCAGAGCTC ACTGGGCTTC AAATCACAGC 1201 CAGATGTGTC CCTGCTGCTG CTGAATTTTC CCACGCTCCC TTTTCCCTCC CAGCTTCTTC 1261 CTCAGGGATA TCCCCCCAGG TTAACCTTCC CTGCCCCAGC TCAGATGCAG AAACTTCTTG 1321 AGAGGATAGG AAATGGTTAC TAGTCAGCTC CGGACTTGGC TTTCCTGGGA ACCTTGGGAT 1381 TGGAGGAAGG AGCCTGCTTC ATTTGGGTCA TATCCAGAAA AGGCCAGAGT CCCAAGGTTT 1441 CCTCAGTCCT CCCTCCTGGC TGATCTCCCA CGACTGTTAT AGGCTGTTTC CCCTGAAAAC 1501 CACCCCATAT GAGTTGGATA AGCTGACATA CCCCCTCCTC CAATCCTTAG TGTGGCCCAG 1561 GCCTACATGA ACCAGAGAAA GCTGGACCAT GAGGTGAAGA CTCTACAGGT CCAGGCTGCC 1621 CAGTTTGCCA AGCAGACAGG CCAGTGGATT GGAATGGTGG AGAACTTCAA CCAGGCACTT 1681 AAGGTGGGCC TCACTCAGTA CATCACTGCC TTTAACTGAA CCGACATTGG CTGTCTCCAG 1741 TCTCATAAAT ATCCCAGACC TAGGGTTAAT GAGGAAGTGG GGATGGTCCC AGATACTCTT 1801 TTCTATGGCT ATAGTATCAG AAAAACTAGA AAGAAAAAGG GCAGGATCTC AGGATATAGC 1861 TCGGTGACAT GTTTAGGGCC CTAGTACATG TCTGGGTTCA ATCCCCAGCA CTACAAAATA 1921 AAAGTACTCA GAAAACCCTG AGTATGGTAC ATGGACCAGT GCTACCAGCT CGTCTGGACG 1981 CTGATGCAGG AGATAGCTTA AGCCCAGGAA TTGAAGCCAA CCTGAGCAAC ACTGCAGTAA 2041 TTCATCTCAA AAGAATGCGA GACAGACAGA AAAGCAGTTG ATGGGTCTGA GTAAGCCACC 2101 CCAGCTGGCC TCAATCTCTT CTCCCCTTTC CCAGGAAATT GGGGGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 62_pAAV-#62-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP62_Rpe65_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label=synP62(Rpe65_2kb) Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTTGCTTG ATCTGTTTCT CCTTTTGATT TATCTATTAC 181 AAATGTCCAT TACAATAATA AGTGAAATGC AAAGTAGATC ATGTAATTCT TCATCAAAAC 241 CTCTTCTTAT GAAGAGCTAT TGATTTCTTT AGATGAGACA AAGTTCCACA AAACTGGACC 301 TTCCCTTATC TCTAGACCAG TCTTAGGTTT ATACTCCTGA GATACAACAC CATGACCAAA 361 AGCAACCTGG AGAGAAAGTT AAATCTTACA GCTCTAGCTC TCAGGTCACA TTTCATCACT 421 GAAGTAAATC AGGGCAGGAA CTCAAGTCAG GAATTTGAAG GCAGGAATTA AAGGAGAGAC 481 CATGAATTGG TGCTGTTTAC CAGGTTGTTC TCCATGGCTT AGTCAGCCTG CTTTCTTATA 541 GCATCCAAGA CCAACTACCT ACTCATGGGT GGCCTGCCCA CAATGGGCTG TGCCCACCCA 601 CTTCAATGGC TAATTAATAA AACGTCCTAC AGACTTGCCT ACAGACCAGT CTAATAGAGG 661 TGTTTTTATC TATTATGGTT CCCTCTTCTC AGATAACTCT TGCTTATATC AAGTTGAAAA 721 ACAAGTTTCA CCTCTTTCTA GTTCATTGTA TCACATTCAC ATCTAAACTT TATAAGCTCT 781 CAAGCTTTGA CTAGCTTTTA TATAGTCTTT CTAGTTCAGA TTGCTATATT TTTCTTTTAG 841 GACTTCCTAA ACCAATGGAG ACATCCCTTC TAAGTACAAC ATCATTTTAT AGAAATTGGA 901 CCCTCAGTTG CAAATGGTGA AACCAACTTT AACTAAGTAA AAAGTGCACA AATAGTTTAA 961 ATACTAGGTA GCTAACATAA TTTCTGGAAT AGTTAAAAGA TAGAGCTTTA GGAATTAACT 1021 GTACAGTTAT TTCCTAAGTT CCTCTACACA GTGTCTTAAG TGGTCTCAGG ACTATCATGA 1081 ATGAGCAGAA TCTCCAATGA GGCCAGGCAC TGGGTGCTAT CACCACCTTC TCCTTTTGCC 1141 TATACTTGTG TCTGTGCCCC TGCTGCAAGT AAGCTGGGAA AGTTCCTCAT ATTTTTCTGA 1201 GTTCTTGGAT TGATTGCTTT ACACAGCTTT GCTGTATAAG GAAAAGTTGT TATAACCATT 1261 TAAATTTAAA TTTATCCCTT AGTTTCCTGA AAGTGATATC ATTGCTCTCT CTTGTCTATT 1321 TTCCTAGGAC TTCTGTAGAC AGCACATGTC TATCCTTAAT AATAGCAGTA GCTTAATATG 1381 AGTGTTGACA TGTGAATAAA ATGGCATTAT TTAGTCTCTG AGTGCAGAAC AAAAGCAATG 1441 GTGAAGACAG TGATGGACAA CAGGCAAGCA GTGGTGATAA GCAAAAACAT GTAGTGTTTC 1501 CTCTTTAATA AGTTCTCAGC TAAAGTTCTC AGCCTTGTTG AAAGGACCTG GATACTGAAC 1561 TGTGCCGAAG AAGGATAGCA GGGTTAAAAC ATGCAAAGAC AGCACCTCAT ATACCTCTAA 1621 TGTTGTTAAC AATAGCTAAC TTTTATCAAA CAGTGTCCTG TCACCATGAC AGTTACAACA 1681 TAATGATAAT GACTGTACTT TCTCTAACCA GGTCTAGATC ACTTATAATA AATATATCTT 1741 TTAGTAATTG AGTAAATGAA TTACAGTGAG GATAACAGCA AAGAAATGGT GGACAGATGT 1801 TTACACCAAG AAAGTATGAT GACTGAGGTC AGCTCAGGAC TGCATGGCAG GCCCACATGG 1861 CTCTTTTTTA TCCAACTCAC TACTCCCTCT CCCTTGAAAG GATCCAAGTC TGGAAAATAG 1921 CCAAAACACT GTTATGTAAA CACCAAGTCC AAATAATGTG CAAGCATCTA AAGTATTGAA 1981 AGCCACTTTT GTTACCTTCC ATCAGCTGAG GGGTGGAGAG GGTTCCCAGA GCCGCAGGCT 2041 CCTCCAATAA GGATTAGATT GCATACAAAA AAGCCCTGGC TAAGAACTTG CTTCCTCATC 2101 CTACAGCTGG TACCAGAACT CTCTCTAATC TTCACTGGAA GAAAGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 64_pAAV-#64-Chr2d-EGFP 6979 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(1418..6979,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..1417 /origin="synP64_mGluR1 (1'284bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 1279^1280, 1'278bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..1416 /label="synP64(mGluR1 )" Restriction_sit join(1417..>1417,complement(1418..>1421),1417^1418,complement(1417^1418)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 1273^1274" /note="cut_0_on_negative_strand: 1277^1278" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 1429..2358 /vntifkey="4" /label=Chr2d CDS 2359..3081 /vntifkey="4" /label=EGFP Misc._feature 3096..3704 /vntifkey="21" /label=WPRE PolyA_signal 3724..4202 /vntifkey="25" /label="hGH polyA" Misc._feature 4242..4382 /vntifkey="21" /label=R-ITR Replication_ori 4474..4780 /vntifkey="33" /label="f1 Origin" Misc._feature 5299..6156 /vntifkey="21" /label=AmpR Replication_ori 6307..6974 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTtcactc tagtgttttc atgtgacctc ttgttctcaa 181 tttagatact tcttgacctg gaaaaggtgg gtgctgcttg ccactctaca tttggctact 241 cctgttttag gatgtgcatg ttccttcctc cccttgtctc ccgccatgaa atctcttccc 301 atcctctttg caacctgaca acagagcctt gtccctaggt agatatacac atacattgtt 361 tttctcattg tgttccttat ttttagagag agaaaaaaaa tctgagtctg taagataaat 421 gcaaaggata acaatcccca aaacagccta ggaacccctc attcctctgg caagacacta 481 ctgatgatct gtatgcttgg tagcctcttt aagtcctctc agagatagta tagtagttga 541 caaatgccag ctcctatatt ctctagcttc tccttttcct ctatcaaatg actttttaaa 601 taaattagat attagacaaa ccttgggtga cctgcttttt cttttctttt cctttttttt 661 ttttttactt ctccccccgc cccccctccc ccccgttttt tttcccttgc ggtataatag 721 tgttgaagaa agagggcact agtgtacagc ccagatggca gccttgcacc gtctgatttg 781 gagctgaggt gtctgcgaac cgagggcggc tgcagtcctc tgacctgaga ccaatagctc 841 tgtctacccg gactcaggta ggtcttgaag atgaaagggt gtgtgtgtgt gtgtgtgtgt 901 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtacgcgcgc gcgcgcgcga gtgtacgaat 961 cctagctctg agcgctgtcc agggttctga catttgagtg gctcaggcgg ttcaagcccc 1021 aacacaggga ggctaccagg cgttgcctta gtaactaata tggcttttcc acaagctgtc 1081 gcatttacat tttccaccat cagcgtccag cttatggcca ctaacgcgcc gctcattgca 1141 cacctgattc acacaccttc gggcaccagt gaaaaaaccg cgacttgatt ttctggaaga 1201 acacccctag ggtttgggag cggtcgtgga ggaccagcag gaagaagcgg aggggagagg 1261 ggcggtggtg gagactgaga agctttgaac cagctgtgtt ggccaaaggc acgaagcggc 1321 aaaagacagc ggcgggcgtc agggtggttc gcgctgggaa cctgcaggcg ggaccggcgt 1381 gggaacgtgg cttgccggcg gtcgaaccgc gtcttcGGAT CCGCCACCAT GGATTATGGA 1441 GGCGCCCTGA GTGCCGTTGG GCGCGAGCTG CTATTTGTAA CGAACCCAGT AGTCGTCAAT 1501 GGCTCTGTAC TTGTGCCTGA GGACCAGTGT TACTGCGCGG GCTGGATTGA GTCGCGTGGC 1561 ACAAACGGTG CCCAAACGGC GTCGAACGTG CTGCAATGGC TTGCTGCTGG CTTCTCCATC 1621 CTACTGCTTA TGTTTTACGC CTACCAAACA TGGAAGTCAA CCTGCGGCTG GGAGGAGATC 1681 TATGTGTGCG CTATCGAGAT GGTCAAGGTG ATTCTCGAGT TCTTCTTCGA GTTTAAGAAC 1741 CCGTCCATGC TGTATCTAGC CACAGGCCAC CGCGTCCAGT GGTTGCGTTA CGCCGAGTGG 1801 CTTCTCACCT GCCCGGTCAT TTGCATTCAC CTGTCAAACC TGACGGGCTT GTCCAACGAC 1861 TACAGCAGGC GCACCATGGG TCTGCTTGTG TCTGATATTG GCACAATTGT GTGGGGCGCC 1921 ACTTCCGCCA TGGCCACCGG ATACGTCAAG GTCATCTTCT TCTGCCTGGG TCTGTGTTAT 1981 GGTGCTAACA CGTTCTTTCA CGCTGCCAAG GCCTACATCG AGGGTTACCA CACCGTGCCG 2041 AAGGGCCGGT GTCGCCAGGT GGTGACTGGC ATGGCTTGGC TCTTCTTCGT ATCATGGGGT 2101 ATGTTCCCCA TCCTGTTCAT CCTCGGCCCC GAGGGCTTCG GCGTCCTGAG CGTGTACGGC 2161 TCCACCGTCG GCCACACCAT CATTGACCTG ATGTCGAAGA ACTGCTGGGG TCTGCTCGGC 2221 CACTACCTGC GCGTGCTGAT CCACGAGCAT ATCCTCATCC ACGGCGACAT TCGCAAGACC 2281 ACCAAATTGA ACATTGGTGG CACTGAGATT GAGGTCGAGA CGCTGGTGGA GGACGAGGCC 2341 GAGGCTGGCG CGGTACCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC 2401 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC 2461 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG 2521 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC 2581 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC 2641 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG 2701 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC 2761 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG 2821 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC 2881 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG 2941 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG 3001 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG 3061 GACGAGCTGT ACAAGTGATA AGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT 3121 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 3181 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 3241 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 3301 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 3361 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 3421 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 3481 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTA TGTTGCCACC 3541 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 3601 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 3661 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGAGCGCT GCTCGAGAGA 3721 TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA 3781 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG 3841 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA 3901 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT 3961 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG 4021 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA 4081 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC 4141 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC 4201 TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA 4261 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC 4321 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA 4381 GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG 4441 TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4501 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 4561 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 4621 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT 4681 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 4741 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC 4801 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 4861 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC 4921 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA 4981 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG 5041 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA 5101 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT 5161 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 5221 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 5281 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 5341 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 5401 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 5461 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 5521 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 5581 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 5641 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 5701 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 5761 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 5821 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 5881 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 5941 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 6001 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 6061 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 6121 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 6181 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 6241 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 6301 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 6361 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 6421 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 6481 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 6541 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 6601 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 6661 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 6721 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 6781 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 6841 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 6901 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 6961 TGGCCTTTTG CTCACATGT // LOCUS 66_pAAV-#66-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP66_Car14_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BclI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label="synP66(Car14_2kb )" Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BclI" /note="Pattern: tgatca" /note="cut_0_on_positive_strand: 2001^2002" /note="cut_0_on_negative_strand: 2005^2006" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BclI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTAAGGAA TCCCAAGTTT TAAAAATTCG TAGAGAAGCC 181 AAGAGGTGGC GCCAGGCGAG ACGCACTTCC TGGGAGGCTG AAAGTCGGTC GTAGATGCTG 241 CAGGGGAGCT CTGTCCTGAT AGGGGTTATT CTATCCAAAC CTCCTCTTTC CTACCTTGTG 301 AAATATGTGT CAATTCTCCT TTCACCCCCA CCCCACCCCC CTCTTAACTT TCCGGACACC 361 AAAGAAATCT TAACTTGTAT TCACATCTTT TATTCTTCCT AGATGGGACT GGATGAGGCT 421 TTGGAAGAAC TTTAAGACGG AAACTTTTTT TTAGGGGATG AAGTATTCGC AAAGCGATAA 481 AACGTTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTATTT TAAATATATA 541 TATATATTTT TAAAAAGTCC TTACTCTGAA GCTCAGTCGT GGGACAGAAA AGCACTCTGA 601 GCTCCTGTCT CCGCAGGAGT GATGACCTGG CACATGCATT ATTCTGTTCA TTTGGCTTTT 661 ATCCCGTGCC CTTGTTTGGC TTCTGCTCCT TTGTTTTGGC TATTTATTGC TACTTGATAG 721 GAGTAAGGAG GGCAGAAAGA CCTCACAGAG GTTATGGAGG AGAGATTTCA CCATCTAGCT 781 TGAAATTATC TGTGGTCTTC ACACTCACTA ATGAGGGACT TAGGATGTAC ATTCCGATCG 841 GGATTTCTAG TCCCGTGGGG GAGCGCACAC CAGCCACATT CCTGAGACGC CACGCAGCCT 901 CCATGCTCCA CTAGCAAACT CAGTTCCACC ACAGCAAGTT TCACTTGATC CCACAGGACC 961 TGCCTGGACC CTTTGTTGGG TGGGCGGGAA GCCCCTGGAC CCCTCACCCA CCTGGTCACA 1021 TGGCCTTTCT ATGGCCCCTC CCCTCTCTTT CTTTGGAGTC CGCCTTTTTC TCATGGTGAC 1081 AGTAATTGTT TCTACAGTCA CCCATCACTC CTCCTGCCCG GCCTCTCTGC TAGGGGTCAG 1141 TGTCCTCTTC AGTGACAAAA GGCGCCCTAG ATCTGGGCTC CCTGGGGAGG GAGGTGGCTG 1201 GATCTGGTAG TTAGAATGCT GTCTCTCCCT GGTGCTGCGA CAACCCCTCC CCCGCTGTGC 1261 ACACAAATGG GCTTTATCCG GCAAACAAAA CCGCACCCCC ACCCCAGGCT ACACAGGCAC 1321 GTGGACCTAA GCCTCACCAA GGGAAGGGGG TGGTGACAGG CGCCAGAGAA AGACGAGGCA 1381 GAAGACAAAT ATTCTCCTCG CCCCACTTCT TGCTCTCCAC AATCCAGATT TTAAAGTTTG 1441 ACTTTCCACT CCCCACCTGC TCCCTGAGCC TCCAACTTCT GTTCTCCTAT CTCCCGAATC 1501 TGAATTCTTT CTGGTTCCAA CCAGCTCAAC TTCTCTTGGT TTCAGAAACA GGTTCCCCAC 1561 CAGCAGTCCC ATACCACCCA GGGATGTGCT CCTCAGGCAG GGGAATGAGA ACACGGTGTC 1621 CTGGGAGGGA GAGGTGCGAA ACCCCCCTTC CCTGACCGAG AGTTCCAGGG GACACCCGTA 1681 CGAGCGGCAG TGTAAATAGC AAAGGAAGAG CTCACTCCAG GGTCCTGAGC CAAGGCCAGG 1741 GCGCTGGGCT CTGCTGGGCC ATCCTGCATG CCTAATCCGC TATTTAAGGA GCTGCTCGGC 1801 TGCAGCCCAG GCACCACCCC CCTCCTCACG TACACCGGTT TCGAGCCACC TTAAAAGCGG 1861 GAGGCAACTG TGAAGTTGCT GGCCAGTAAG TGGTGTGGAG ATTACGAAGG GACATACGGA 1921 GAGGGCAGAG AGAGGAAGAG AGAGAAAGTG AGAGAGGAAG AAATTTATCC TGGGATCCAG 1981 AAGTTCGTGT TAACCTGTGG AAAATCAAGT CCCTGGAAGT CTGCAGAGAC AGAGACAAGA 2041 AGAAAAGAGA TAGAACGCCA GAAACTCCTC TCTCTCCCTC CCTCTCCACC TCTCTCTTCT 2101 AAACCCCAAA TTCCTGGTCC CTTGTACCCC ATTGTGGGGA TAATTGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT // LOCUS 68_pAAV-#68-Chr2d-EGFP 7707 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Vector join(2146..7707,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Misc._feature 1..130 /vntifkey="21" /label=L-ITR Insertion 140..2145 /origin="synP68_Cp_2kb (2'012bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 2007^2008, 2'006bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 145..2144 /label="synP68(Cp_2kb )" Restriction_sit join(2145..>2145,complement(2146..>2149),2145^2146,complement(2145^2146)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 2007^2008" /note="cut_0_on_negative_strand: 2011^2012" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 2157..3086 /vntifkey="4" /label=Chr2d CDS 3087..3809 /vntifkey="4" /label=EGFP Misc._feature 3824..4432 /vntifkey="21" /label=WPRE PolyA_signal 4452..4930 /vntifkey="25" /label="hGH polyA" Misc._feature 4970..5110 /vntifkey="21" /label=R-ITR Replication_ori 5202..5508 /vntifkey="33" /label="f1 Origin" Misc._feature 6027..6884 /vntifkey="21" /label=AmpR Replication_ori 7035..7702 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTGAAGAA GTTTCTTACA AAACATGGAG CTTGACCAAT 181 GAAGTTTGGA CAAAATTTCA GACATACTCC TGTCCTCCCT GTGGATGTGA AGGAAGAGTT 241 GTTCAGGAAA GTTCCAGAAA GAGGCTTGGG CACCTCGCCT CCTATAATTC CCTTTTCTGT 301 ATAAAGTCAA GTGGTCAAGA TTATCAGTAG CAAGGATAGC CCAGTAGCTC AGTACTAGAG 361 CACTTGCCTG TTAAACATAA GGATGCCTAA ATCTCTGTAT TCAATCCCAT TAAAACACAC 421 ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACTGTT GTTAGTAGCT 481 TGCAAGGTCA TTTCAATTGA TCTGGATAAT TTCCTTCAAG GCTGACCTTT ACAAATGAGG 541 AAACTGAAGC TAAGGGTAAG AATTTTGACA GCATCAGATT TTAACTTTAT CCTGACATTT 601 TTTTTCTAGC ATCTATGACT GACATTGTGT TCCTCATTTT TCATGTGTTA ATAAGTTTTC 661 ATGTTGTATA TAACGGCATG CATGCTCCAT GGAGTTATAT CCACTGCTAT CTGCCCACTG 721 AGGACAGAAC TGCACGCACC ATGGAACTCA TTCAATATTT GTTAAATAAG AGTACCTGTT 781 CTGATACCAG TCTATTTCAG CAAATAGCAT TTAGTGCTAT ACAGTATCCT ACAAGAAAAC 841 AACAATAACA ACAATCGCTT TATTAACACA AAATAGTAAC TGCTATCATC AGGATTGCAT 901 TTCACTTTAG GAAAAAAAAA TTATACATTT AGATACATGC CACAAGTGAA GACAGAGTTC 961 TAACCAAAAA TCTTTTACCT TAAAGCTCTC ATACATGTTA CAGAGAGAAA TTCTAGGTTA 1021 ATAAACAAAG CTCTTTTTCC AGTTGAGTAA AATCATTCTT TGCCTTTGAA AACAGGCTTA 1081 ATTGTGGTTG ATTTTTTTCT TTTCCTCTCT CTTTCCTATC TCTTTTCCTT TCTCTCCTCC 1141 TCTTCCTTCT TTGCCCATTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 1201 TCTCTCTGAG TATTACTTCA TTGTACAGAC GGGTACTAAA ATCCTAGATT CAACAGATTG 1261 TCATGTGCCA GTTTCCCCAT TATGCATAGC TTATTTGGTT TGTGACTGAC TTCTTTTTTT 1321 TTTTTAAAGA TTTATTTATT ATTATACATA AGTTCACTGT AGCTGACTTC AGACACACCA 1381 TAAGAGGGTG TCAGATCTCA TTACAGGTGG TTGTGAGCCA CCATGTGGTT GCTGGGATTT 1441 GAACTCAGGA CCTTCAGAAG AGCAGTCAGT GCTCTTATCC GCTAAGCCAT CTCACCAGCC 1501 CTTGTGACTG ACTTCTATCA TTCAAACAAA ATTATCATTT CCTGGTATGT TCTTCTATTA 1561 ATTTCTACAT AGCAATCCAT GTCAACTGAT AAAGTCTCTA TAACACACAG TACAAAAAAA 1621 TTTTTTTAAA TGAAATTTTT ATTGGCTACT TTTACGATGT GCTAAATATG TTTCATATCT 1681 TCAAAATTAC AAGTAAGTTT TTGTTAGTTT TCTAAAAATC ATTAACAATG ACATATTTGA 1741 TTCATTTTTC CTTGGCTTAT TAAAAACTGG CTCTGGGTGA GGTGTAGTGC CTGGGATTGC 1801 TAGAAGAACA AAGGGAGCAT TTAGAGATGG TCTCTGCCCT CCTGGAATTT ACACGATGAA 1861 TGGAAGAAGA TGCAGAATTG CGCATGCTGT GCATGTCCAA CACAAACAGA TGACATTCCT 1921 TTGTAGTTAG AGAAGCCTGG CCACAGGGAA CTGTGTTTGT TTTGGTTTTT CCCCTTCGTG 1981 GTTTACCAGA ACCAATCTGA GTTGGAAAAT GCTATTTGCT TTAAAGAAAT TAGTTACTCT 2041 TTTGCAACAT TAAATTGTGT CAGCCAAGCA AATGAAGCCC TATTTATAAA ATTGCTTGCT 2101 TCTGGTTTCA CTTTATTGCA TCGTGGGCTC CGAGAGGGAG AAAAGGATCC GCCACCATGG 2161 ATTATGGAGG CGCCCTGAGT GCCGTTGGGC GCGAGCTGCT ATTTGTAACG AACCCAGTAG 2221 TCGTCAATGG CTCTGTACTT GTGCCTGAGG ACCAGTGTTA CTGCGCGGGC TGGATTGAGT 2281 CGCGTGGCAC AAACGGTGCC CAAACGGCGT CGAACGTGCT GCAATGGCTT GCTGCTGGCT 2341 TCTCCATCCT ACTGCTTATG TTTTACGCCT ACCAAACATG GAAGTCAACC TGCGGCTGGG 2401 AGGAGATCTA TGTGTGCGCT ATCGAGATGG TCAAGGTGAT TCTCGAGTTC TTCTTCGAGT 2461 TTAAGAACCC GTCCATGCTG TATCTAGCCA CAGGCCACCG CGTCCAGTGG TTGCGTTACG 2521 CCGAGTGGCT TCTCACCTGC CCGGTCATTT GCATTCACCT GTCAAACCTG ACGGGCTTGT 2581 CCAACGACTA CAGCAGGCGC ACCATGGGTC TGCTTGTGTC TGATATTGGC ACAATTGTGT 2641 GGGGCGCCAC TTCCGCCATG GCCACCGGAT ACGTCAAGGT CATCTTCTTC TGCCTGGGTC 2701 TGTGTTATGG TGCTAACACG TTCTTTCACG CTGCCAAGGC CTACATCGAG GGTTACCACA 2761 CCGTGCCGAA GGGCCGGTGT CGCCAGGTGG TGACTGGCAT GGCTTGGCTC TTCTTCGTAT 2821 CATGGGGTAT GTTCCCCATC CTGTTCATCC TCGGCCCCGA GGGCTTCGGC GTCCTGAGCG 2881 TGTACGGCTC CACCGTCGGC CACACCATCA TTGACCTGAT GTCGAAGAAC TGCTGGGGTC 2941 TGCTCGGCCA CTACCTGCGC GTGCTGATCC ACGAGCATAT CCTCATCCAC GGCGACATTC 3001 GCAAGACCAC CAAATTGAAC ATTGGTGGCA CTGAGATTGA GGTCGAGACG CTGGTGGAGG 3061 ACGAGGCCGA GGCTGGCGCG GTACCCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG 3121 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 3181 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 3241 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 3301 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 3361 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 3421 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 3481 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 3541 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 3601 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 3661 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 3721 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 3781 TCGGCATGGA CGAGCTGTAC AAGTGATAAG AATTCGATAT CAAGCTTATC GATAATCAAC 3841 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 3901 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 3961 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4021 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4081 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4141 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4201 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTATG 4261 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4321 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4381 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGAGCGCTGC 4441 TCGAGAGATC TACGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA 4501 AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC 4561 TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA 4621 AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT 4681 GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA 4741 GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT 4801 TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT 4861 GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT 4921 CCCTGTCCTT CTGATTTTGT AGGTAACCAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA 4981 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA 5041 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC 5101 TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC 5161 CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5221 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5281 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5341 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5401 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5461 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 5521 TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 5581 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 5641 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC 5701 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC 5761 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 5821 GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 5881 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 5941 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 6001 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 6061 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 6121 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 6181 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 6241 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 6301 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 6361 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 6421 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 6481 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 6541 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 6601 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 6661 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 6721 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 6781 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 6841 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 6901 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 6961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 7021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 7081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 7141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 7201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 7261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 7321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 7381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 7441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 7501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 7561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 7621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 7681 CCTTTTGCTG GCCTTTTGCT CACATGT //