LOCUS       51_pAAV-synP51-Chr2d-EGFP      7421 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(1860..7421,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..1859
                     /origin="51 (1'726bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 1721^1722, 1'720bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             1871..2800
                     /vntifkey="4"
                     /label=Chr2d
     CDS             2801..3523
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   3538..4146
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    4166..4644
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   4684..4824
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 4916..5222
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   5741..6598
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 6749..7416
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTTCAAGC CTCCTACCGC CTTTGTTATG CAAACATATC
      181 AAACGCCCTC CTTTGTTATG CAAAAGGGCT GGAACGGGGC CTTTGTTATG CAAATCGCCC
      241 TCCCCGATCC CTTTGTTATG CAAATTTGAC GAATTCCCAC CTTTGTTATG CAAACAAATC
      301 TCCTACCCTC CTTTGTTATG CAAAGTGAGA GGGGCTGCAC CTTTGTTATG CAAAATGCGG
      361 CCCCTGAGAC CTTTGTTATG CAAAAACCAT GTACGCTTGC CTTTGTTATG CAAACCGCCT
      421 GTTGCTTGGC CTTTGTTATG CAAAGCCACG CGATTGGCGC CTTTGTTATG CAAAGCTCGG
      481 TTATGTACAC CTTTGTTATG CAAAGCTACT TTAAACTTGC CTTTGTTATG CAAATCACGA
      541 CCTGACCGTC CTTTGTTATG CAAAAACGGT TGAAATAGTC CTTTGTTATG CAAAATGATA
      601 TTGAATAGTC CTTTGTTATG CAAAAATTTA GATGCCGACC CTTTGTTATG CAAAGGAATG
      661 GGCGTGCTGC CTTTGTTATG CAAATTTTCG CTGCGACAGC CTTTGTTATG CAAACATGCT
      721 CGCCACTCAC CTTTGTTATG CAAAGGTCTA ACAATGACCC CTTTGTTATG CAAACTACGT
      781 GGAATAGATC CTTTGTTATG CAAACCCCGA GTTTTTGAAC CTTTGTTATG CAAAATCAGT
      841 AACTTCATTC CTTTGTTATG CAAAATGTGA CTTAACCTCC CTTTGTTATG CAAATACACG
      901 CTAGTCGCAT GGACGTGGAT GCTAGCTAAA ACCCAGCAGG CTCGGTTGTT GTAGTTCTTA
      961 CATAATGACT ATCACCTCGG CAAGGGAGTT TTAAGCGGCA GCGGGAGAAT TGCCGCATGA
     1021 ACACAAACTT TCTCTGTCGA TGGTTCGCGG AACTCTTCGG CGATTGTCCG CCCCTTTCAA
     1081 CCACCTAAAT AGATGTGCTT TTTGGGTTGA GGTGCTGTTC CAAAGAACTG GCACGCATCG
     1141 AAGCACGTCA AAATCGGCTC GTAGAAATTC GCACGTATTC CCCGTGCCTG GTGGGTCGTC
     1201 GAGATTCTGT GAAGGAGCAC TGGGAACGAA TGCGTGGGTC ACCATACCCG TAGAATGCTT
     1261 GTAACGCCAC TAGCGGCTTG GTCATCTTTC CCTAAAGGCT GCGTGGTCCC GAATGACGTA
     1321 ACGTCGATGG CCGGTTAGCC CAAAAATATG CCCGTATTTA CTAAACCTGT CGTGTCTCTC
     1381 TTCCTGTTGT CTCTCGCTAG GTCCATCCCT AAGTCTTATC GAAGGTGCTA TTTTACTCTG
     1441 CTCTTGGAAC ACACCCATCT CCACGCACGT GATAGGTACA GCAAGCGTTA CAGGCCATCG
     1501 TTTCGTACGA ATTTTCGAAA AGTAATCCTC AGAAATCGAA TATTACCTTT AGTTGGAACG
     1561 CGCTTCCAAA GCGCCGGGCC CTGAAGTGCT ATGGGATGGG CTTCTTTCGC GTGTCCTTTT
     1621 GGCCGATGTC TGACTGACAG ATTAGCTCGA GATCTGCGAT CTGCATCTCA ATTAGTCAGC
     1681 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA
     1741 TTCTCCGCCC CATCGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC
     1801 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGG
     1861 ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT GGGCGCGAGC TGCTATTTGT
     1921 AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT GAGGACCAGT GTTACTGCGC
     1981 GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG GCGTCGAACG TGCTGCAATG
     2041 GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC GCCTACCAAA CATGGAAGTC
     2101 AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG ATGGTCAAGG TGATTCTCGA
     2161 GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA GCCACAGGCC ACCGCGTCCA
     2221 GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC ATTTGCATTC ACCTGTCAAA
     2281 CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG GGTCTGCTTG TGTCTGATAT
     2341 TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC GGATACGTCA AGGTCATCTT
     2401 CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT CACGCTGCCA AGGCCTACAT
     2461 CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG GTGGTGACTG GCATGGCTTG
     2521 GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC ATCCTCGGCC CCGAGGGCTT
     2581 CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC ATCATTGACC TGATGTCGAA
     2641 GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG ATCCACGAGC ATATCCTCAT
     2701 CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT GGCACTGAGA TTGAGGTCGA
     2761 GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC ATGGTGAGCA AGGGCGAGGA
     2821 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
     2881 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
     2941 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
     3001 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
     3061 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
     3121 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
     3181 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
     3241 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
     3301 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
     3361 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
     3421 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
     3481 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA TAAGAATTCG ATATCAAGCT
     3541 TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA
     3601 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC
     3661 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA
     3721 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC
     3781 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC
     3841 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC
     3901 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG
     3961 GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC
     4021 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC
     4081 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG
     4141 ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT
     4201 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT
     4261 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA
     4321 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA
     4381 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG
     4441 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC
     4501 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC
     4561 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA
     4621 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC
     4681 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG
     4741 AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG
     4801 AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT
     4861 GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT
     4921 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
     4981 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
     5041 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
     5101 CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT
     5161 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
     5221 AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
     5281 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
     5341 AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
     5401 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
     5461 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC
     5521 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
     5581 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     5641 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     5701 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     5761 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     5821 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     5881 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     5941 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     6001 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     6061 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT
     6121 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
     6181 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT
     6241 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC
     6301 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
     6361 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
     6421 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT
     6481 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
     6541 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA
     6601 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     6661 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     6721 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     6781 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     6841 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     6901 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     6961 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     7021 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     7081 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     7141 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     7201 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     7261 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     7321 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     7381 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG T
//
