LOCUS       109_pAAV-synP109-Chr2d-EGFP      6381 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(820..6381,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..819
                     /origin="109 (686bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 681^682, 680bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             831..1760
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1761..2483
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2498..3106
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3126..3604
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3644..3784
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3876..4182
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4701..5558
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5709..6376
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTCGCTTT CCAGTATGCC ACCTGACGAT TGTTCACCGG
      181 TCCACCTGAC CTCTCCCGGT CGCCTCACCT GACAGACATC GGCGGTGTCA CCTGACTGAT
      241 AGATGCGATT CCACCTGACA CGATACGCAG CTAGCACCTG ACCCCACAAG ACTAACACAC
      301 CTGACGATGA TTCACCTTGC CACCTGACGC GCTTGCCCAC AGTCACCTGA CGAACTTTGC
      361 CGGGAACACC TGACTGGAAC CCGGCACACC ACCTGACCGA CACGCCATCG ACCACCTGAC
      421 CCATTGATAG GGATACACCT GACTAATTTT TCGAATTTCA CCTGACATAC TTTTGTTGAA
      481 TCACCTGACT CGCTCTATAG TCAGCACCTG ACATATTTGA GTCCTCACAC CTGACCCAAC
      541 CAGGTACCCA CACCTGACCA AAGCATATGG CAACACCTGA CAATACACTC GCGGGACACC
      601 TGACGCTCGA GATCTGCGAT CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
      661 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC
      721 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
      781 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG
      841 GAGGCGCCCT GAGTGCCGTT GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA
      901 ATGGCTCTGT ACTTGTGCCT GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG
      961 GCACAAACGG TGCCCAAACG GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA
     1021 TCCTACTGCT TATGTTTTAC GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA
     1081 TCTATGTGTG CGCTATCGAG ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA
     1141 ACCCGTCCAT GCTGTATCTA GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT
     1201 GGCTTCTCAC CTGCCCGGTC ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG
     1261 ACTACAGCAG GCGCACCATG GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG
     1321 CCACTTCCGC CATGGCCACC GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT
     1381 ATGGTGCTAA CACGTTCTTT CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC
     1441 CGAAGGGCCG GTGTCGCCAG GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG
     1501 GTATGTTCCC CATCCTGTTC ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG
     1561 GCTCCACCGT CGGCCACACC ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG
     1621 GCCACTACCT GCGCGTGCTG ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA
     1681 CCACCAAATT GAACATTGGT GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG
     1741 CCGAGGCTGG CGCGGTACCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
     1801 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
     1861 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC
     1921 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC
     1981 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
     2041 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
     2101 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
     2161 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
     2221 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG
     2281 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
     2341 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG
     2401 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
     2461 TGGACGAGCT GTACAAGTGA TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG
     2521 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
     2581 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
     2641 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA
     2701 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG
     2761 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG
     2821 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA
     2881 ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA
     2941 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC
     3001 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC
     3061 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA
     3121 GATCTACGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC
     3181 CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA
     3241 GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG
     3301 GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA
     3361 ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC
     3421 CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA
     3481 GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA
     3541 CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT
     3601 CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG
     3661 GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC
     3721 GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG
     3781 CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
     3841 CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     3901 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     3961 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     4021 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG
     4081 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     4141 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     4201 GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     4261 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT
     4321 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA
     4381 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
     4441 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA
     4501 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
     4561 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
     4621 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     4681 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     4741 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     4801 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     4861 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     4921 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     4981 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     5041 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     5101 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     5161 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     5221 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     5281 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     5341 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     5401 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     5461 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     5521 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     5581 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     5641 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     5701 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     5761 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     5821 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     5881 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     5941 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     6001 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     6061 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     6121 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     6181 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     6241 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     6301 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     6361 GCTGGCCTTT TGCTCACATG T
//
