LOCUS       113_pAAV-synP113-Chr2d-EGFP      6421 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(860..6421,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..859
                     /origin="113 (726bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 721^722, 720bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             871..1800
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1801..2523
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2538..3146
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3166..3644
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3684..3824
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3916..4222
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4741..5598
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5749..6416
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTTATGGC TAGTGCTCAG ACCACCCAGT CGCGGACGGC
      181 CATGGACCAC CCAGTCCTAT TAACAAAGCG ACCACCCAGC TCACTAGAGT AGAGGACCAC
      241 CCAGCTATAG TAATCTACAG ACCACCCAGA TGGGTACTCT GGGGGACCAC CCAGACCAAA
      301 TTCATCGAGG ACCACCCAGG CAACGTCCAC TTCTGACCAC CCAGAAATGG AGAAAGTGTG
      361 ACCACCCAGT CACCGCACTC GTAAGACCAC CCAGAGCCGT GTCCGCCTGG ACCACCCAGC
      421 TCACTATGAA TTTTGACCAC CCAGAACTAG TAAGTGTAGG ACCACCCAGC CCGTAGCTGT
      481 TCATGACCAC CCAGGGCCGG AGGGGTCCCG ACCACCCAGC CTAACGCCTA CATCGACCAC
      541 CCAGAAATCT TTGACCAAGG ACCACCCAGA GTGCGCACCA ATCTGACCAC CCAGTCCAAC
      601 TTACAACCGG ACCACCCAGG GTCAGGTAGT GAGAGACCAC CCAGGCTCGA GATCTGCGAT
      661 CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
      721 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC TAATTTTTTT TATTTATGCA
      781 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
      841 GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT
      901 GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT
      961 GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG
     1021 GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC
     1081 GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG
     1141 ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA
     1201 GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC
     1261 ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG
     1321 GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC
     1381 GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT
     1441 CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG
     1501 GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC
     1561 ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC
     1621 ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG
     1681 ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT
     1741 GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC
     1801 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1861 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     1921 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     1981 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     2041 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2101 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2161 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2221 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
     2281 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
     2341 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2401 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2461 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA
     2521 TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA
     2581 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG
     2641 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC
     2701 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC
     2761 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT
     2821 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT
     2881 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG
     2941 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG
     3001 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG
     3061 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT
     3121 TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT
     3181 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC
     3241 TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA
     3301 TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT
     3361 GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT
     3421 CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG
     3481 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT
     3541 TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT
     3601 TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA
     3661 CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
     3721 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
     3781 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA
     3841 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC
     3901 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT
     3961 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG
     4021 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
     4081 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA
     4141 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
     4201 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA
     4261 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     4321 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC
     4381 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
     4441 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC
     4501 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA
     4561 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT
     4621 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
     4681 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     4741 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
     4801 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
     4861 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
     4921 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
     4981 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
     5041 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
     5101 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
     5161 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
     5221 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
     5281 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     5341 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     5401 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     5461 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     5521 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     5581 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     5641 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     5701 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     5761 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     5821 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     5881 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     5941 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     6001 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     6061 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     6121 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     6181 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     6241 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     6301 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     6361 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     6421 T
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