LOCUS 115_pAAV-synP115-Chr2d-EGFP 6541 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(980..6541,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Insertion 140..979 /origin="115 (846bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 841^842, 840bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert CDS 991..1920 /vntifkey="4" /label=Chr2d CDS 1921..2643 /vntifkey="4" /label=EGFP Misc._feature 2658..3266 /vntifkey="21" /label=WPRE PolyA_signal 3286..3764 /vntifkey="25" /label="hGH polyA" Misc._feature 3804..3944 /vntifkey="21" /label=R-ITR Replication_ori 4036..4342 /vntifkey="33" /label="f1 Origin" Misc._feature 4861..5718 /vntifkey="21" /label=AmpR Replication_ori 5869..6536 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTCGCGGT TATTCCCCTA TGGGGTATCA ATAATTTTCG 181 GGCCAACTGC ATGGGGTATC AATAATTCAA TTTTGTAGTA TATGGGGTAT CAATAATTGG 241 TCTCAGTATT AAATGGGGTA TCAATAATTC GAACCAATAC GGCATGGGGT ATCAATAATC 301 AATCCCAGGC CGTAATGGGG TATCAATAAT TGGGATTAGC GCTCTATGGG GTATCAATAA 361 TCCGGCGTAC CGATCAATGG GGTATCAATA ATAAAGGGAT TATTCACATG GGGTATCAAT 421 AATACCAGCA AATGAGACAT GGGGTATCAA TAATCAAGTA GATGTCAGTA TGGGGTATCA 481 ATAATTCAAC ACACTGATGA ATGGGGTATC AATAATGCTT AGATGTATCC TATGGGGTAT 541 CAATAATTGA CGCAACAACT CTATGGGGTA TCAATAATGG GACCGTAGGA TTCATGGGGT 601 ATCAATAATA TCTAAATCTT GGCGATGGGG TATCAATAAT GGTTTCTATC AGACAATGGG 661 GTATCAATAA TAAAAGGTAC TGGGCGATGG GGTATCAATA ATGTCCCTAG GCACCATATG 721 GGGTATCAAT AATGATCGCA GCCGGTTCAT GGGGTATCAA TAATGCTCGA GATCTGCGAT 781 CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 841 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC TAATTTTTTT TATTTATGCA 901 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 961 GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT 1021 GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT 1081 GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG 1141 GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC 1201 GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG 1261 ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA 1321 GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC 1381 ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG 1441 GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC 1501 GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT 1561 CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG 1621 GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC 1681 ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC 1741 ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG 1801 ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT 1861 GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC 1921 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 1981 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC 2041 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC 2101 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG 2161 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 2221 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG 2281 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC 2341 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC 2401 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC 2461 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC 2521 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC 2581 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA 2641 TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 2701 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 2761 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 2821 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 2881 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 2941 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 3001 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 3061 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG 3121 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 3181 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 3241 TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT 3301 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC 3361 TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA 3421 TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT 3481 GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT 3541 CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG 3601 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT 3661 TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT 3721 TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA 3781 CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT 3841 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC 3901 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA 3961 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC 4021 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 4081 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 4141 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4201 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA 4261 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 4321 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA 4381 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 4441 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC 4501 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA 4561 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC 4621 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA 4681 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT 4741 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 4801 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT 4861 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 4921 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA 4981 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 5041 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC 5101 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG 5161 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 5221 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC 5281 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 5341 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 5401 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT 5461 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 5521 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT 5581 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 5641 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 5701 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT 5761 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG 5821 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC 5881 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 5941 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG 6001 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA 6061 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA 6121 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG 6181 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 6241 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG 6301 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG 6361 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC 6421 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA 6481 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 6541 T //