LOCUS       116_pAAV-synP116-Chr2d-EGFP      6361 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(800..6361,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..799
                     /origin="116 (666bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 661^662, 660bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             811..1740
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1741..2463
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2478..3086
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3106..3584
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3624..3764
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3856..4162
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4681..5538
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5689..6356
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTAATCGG GAACACCTAA AAACAAATTT GAAGGGCACA
      181 CAAAACAAAT GCCAGACCCC TCAAAAACAA GTCACGTAAC GGGCTAAAAC AACCATGTAG
      241 ATGTGTTAAA ACAACGGTGC TGTAGGCCCA AAACAAACTC AACTCACGAT GAAAACAAAT
      301 CCAACGGCTG GTCAAAACAA TCACGGGATC ACTCAAAAAC AAAAGAGGTC GGGGGGTAAA
      361 ACAAAGTGAA TGATTTCGCA AAACAAGGTT CGTGTCTGCG CAAAACAATT TGAGACATAA
      421 TTCAAAACAA GACACGGGTG AATGAAAAAC AAAAATTCCA ACCTGGGAAA ACAACCCATG
      481 CAATATTTAA AAACAAGCCA ATTGCTGGTT TAAAACAAGG GGTGGGTAGC CCGAAAACAA
      541 GCAATGGACG TTTTAAAAAC AATGCCTGCC GCTGACAAAA ACAAGCTCGA GATCTGCGAT
      601 CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
      661 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC TAATTTTTTT TATTTATGCA
      721 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
      781 GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT
      841 GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT
      901 GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG
      961 GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC
     1021 GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG
     1081 ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA
     1141 GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC
     1201 ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG
     1261 GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC
     1321 GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT
     1381 CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG
     1441 GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC
     1501 ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC
     1561 ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG
     1621 ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT
     1681 GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC
     1741 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1801 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     1861 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     1921 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     1981 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2041 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2101 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2161 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
     2221 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
     2281 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2341 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2401 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA
     2461 TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA
     2521 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG
     2581 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC
     2641 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC
     2701 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT
     2761 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT
     2821 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG
     2881 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG
     2941 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG
     3001 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT
     3061 TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT
     3121 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC
     3181 TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA
     3241 TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT
     3301 GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT
     3361 CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG
     3421 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT
     3481 TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT
     3541 TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA
     3601 CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
     3661 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
     3721 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA
     3781 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC
     3841 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT
     3901 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG
     3961 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
     4021 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA
     4081 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
     4141 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA
     4201 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     4261 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC
     4321 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
     4381 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC
     4441 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA
     4501 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT
     4561 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
     4621 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     4681 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
     4741 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
     4801 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
     4861 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
     4921 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
     4981 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
     5041 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
     5101 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
     5161 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
     5221 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     5281 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     5341 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     5401 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     5461 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     5521 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     5581 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     5641 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     5701 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     5761 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     5821 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     5881 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     5941 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     6001 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     6061 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     6121 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     6181 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     6241 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     6301 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     6361 T
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