LOCUS       151_pAAV-synP151-Chr2d-EGFP      6481 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(920..6481,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..919
                     /origin="151 (786bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 781^782, 780bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             931..1860
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1861..2583
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2598..3206
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3226..3704
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3744..3884
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3976..4282
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4801..5658
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5809..6476
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTCTTACC GATTGCAGAC GAAACCGAAA CTTAGCTGAC
      181 ATCGAGTCGA AACCGAAACT TTTCATGGCC GAAGGCGAAA CCGAAACTTG CGTCCGTGTA
      241 ACGCGAAACC GAAACTTCCC GAGCATCCTT ACGAAACCGA AACTGCAACC GGCTACACTC
      301 GAAACCGAAA CTACTAATGA TACCAACCGA AACCGAAACT CGGTAAGTGA AACTGCGAAA
      361 CCGAAACTTG GTGGACAGCC TTCCGAAACC GAAACTGGAC GATGTTCGTT CCGAAACCGA
      421 AACTATATTC GTGCCCAAGC GAAACCGAAA CTAGGGGGCC GATATCCCGA AACCGAAACT
      481 ACAGGCCCCG CCCGTCGAAA CCGAAACTTA CGTCTCAGGA AGTCGAAACC GAAACTGGCT
      541 ACCACTGACC ACGAAACCGA AACTTCTACT GTTCCGTGAC GAAACCGAAA CTGACGGACA
      601 AGAGTACCGA AACCGAAACT TAGTCAGGCG TCCATCGAAA CCGAAACTCA AAGATTCAAA
      661 AAACGAAACC GAAACTTTCT GTTGGGATGT CCGAAACCGA AACTGCTCGA GATCTGCGAT
      721 CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
      781 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC TAATTTTTTT TATTTATGCA
      841 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
      901 GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT
      961 GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT
     1021 GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG
     1081 GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC
     1141 GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG
     1201 ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA
     1261 GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC
     1321 ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG
     1381 GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC
     1441 GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT
     1501 CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG
     1561 GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC
     1621 ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC
     1681 ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG
     1741 ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT
     1801 GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC
     1861 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1921 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     1981 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     2041 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     2101 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2161 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2221 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2281 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
     2341 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
     2401 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2461 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2521 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA
     2581 TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA
     2641 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG
     2701 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC
     2761 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC
     2821 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT
     2881 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT
     2941 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG
     3001 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG
     3061 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG
     3121 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT
     3181 TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT
     3241 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC
     3301 TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA
     3361 TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT
     3421 GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT
     3481 CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG
     3541 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT
     3601 TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT
     3661 TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA
     3721 CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
     3781 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
     3841 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA
     3901 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC
     3961 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT
     4021 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG
     4081 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
     4141 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA
     4201 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
     4261 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA
     4321 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     4381 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC
     4441 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
     4501 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC
     4561 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA
     4621 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT
     4681 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
     4741 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     4801 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
     4861 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
     4921 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
     4981 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
     5041 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
     5101 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
     5161 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
     5221 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
     5281 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
     5341 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     5401 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     5461 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     5521 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     5581 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     5641 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     5701 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     5761 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     5821 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     5881 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     5941 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     6001 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     6061 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     6121 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     6181 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     6241 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     6301 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     6361 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     6421 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     6481 T
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