LOCUS       152_pAAV-synP152-Chr2d-EGFP      6441 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(880..6441,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5'701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Insertion       140..879
                     /origin="152 (746bp linear)          Fragment: from MluI
                     cut at 1^2 to BamHI cut at 741^742, 740bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     CDS             891..1820
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1821..2543
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2558..3166
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3186..3664
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3704..3844
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3936..4242
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4761..5618
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5769..6436
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTTCAAGT TCATTAGCGA TTGCACAATA TCAGAGAACC
      181 TTAGTATTGC ACAATATATT CGTTCTCAAG CATTGCACAA TAACGTATTT GGTCACCATT
      241 GCACAATACC GATTAGAACG CAAATTGCAC AATAAGACAT CCTTCTGTCA TTGCACAATA
      301 TAAGCTGATT ACAATATTGC ACAATACATC CTTCGTATAT GATTGCACAA TAGGCGTGGC
      361 GCATGCAATT GCACAATAGT TTCAATTGAG GGCATTGCAC AATATATCGC GCGAGCCGAA
      421 TTGCACAATA CCAGCAGAAA TACGCATTGC ACAATATACT AATGACAGCC GATTGCACAA
      481 TACCTACTTG ATAGGGTATT GCACAATAAT AAACTCACAT GATATTGCAC AATAATCTCA
      541 AGACGCGGAA TTGCACAATA GTCTTGCACA GACGCATTGC ACAATAGTTA CAGTCTTGAG
      601 TATTGCACAA TAAGAAAGAC AACAAGTATT GCACAATAGG TTAATTGGTT AGCATTGCAC
      661 AATAGCTCGA GATCTGCGAT CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
      721 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATCGCTGAC
      781 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
      841 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGG ATCCGCCACC ATGGATTATG
      901 GAGGCGCCCT GAGTGCCGTT GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA
      961 ATGGCTCTGT ACTTGTGCCT GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG
     1021 GCACAAACGG TGCCCAAACG GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA
     1081 TCCTACTGCT TATGTTTTAC GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA
     1141 TCTATGTGTG CGCTATCGAG ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA
     1201 ACCCGTCCAT GCTGTATCTA GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT
     1261 GGCTTCTCAC CTGCCCGGTC ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG
     1321 ACTACAGCAG GCGCACCATG GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG
     1381 CCACTTCCGC CATGGCCACC GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT
     1441 ATGGTGCTAA CACGTTCTTT CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC
     1501 CGAAGGGCCG GTGTCGCCAG GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG
     1561 GTATGTTCCC CATCCTGTTC ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG
     1621 GCTCCACCGT CGGCCACACC ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG
     1681 GCCACTACCT GCGCGTGCTG ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA
     1741 CCACCAAATT GAACATTGGT GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG
     1801 CCGAGGCTGG CGCGGTACCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
     1861 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
     1921 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC
     1981 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC
     2041 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
     2101 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
     2161 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
     2221 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
     2281 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG
     2341 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
     2401 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG
     2461 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
     2521 TGGACGAGCT GTACAAGTGA TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG
     2581 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
     2641 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
     2701 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA
     2761 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG
     2821 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG
     2881 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA
     2941 ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA
     3001 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC
     3061 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC
     3121 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA
     3181 GATCTACGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC
     3241 CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA
     3301 GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG
     3361 GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA
     3421 ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC
     3481 CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA
     3541 GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA
     3601 CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT
     3661 CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG
     3721 GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC
     3781 GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG
     3841 CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
     3901 CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     3961 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     4021 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     4081 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG
     4141 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     4201 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     4261 GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     4321 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT
     4381 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA
     4441 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
     4501 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA
     4561 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
     4621 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
     4681 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     4741 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     4801 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     4861 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     4921 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     4981 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     5041 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     5101 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     5161 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     5221 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     5281 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     5341 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     5401 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     5461 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     5521 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     5581 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     5641 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     5701 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     5761 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     5821 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     5881 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     5941 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     6001 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     6061 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     6121 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     6181 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     6241 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     6301 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     6361 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     6421 GCTGGCCTTT TGCTCACATG T
//
