LOCUS 171_pAAV-synP171-Chr2d-EGFP 6693 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(1132..6693,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Insertion 140..1131 /origin="171 (998bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 993^994, 992bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert CDS 1143..2072 /vntifkey="4" /label=Chr2d CDS 2073..2795 /vntifkey="4" /label=EGFP Misc._feature 2810..3418 /vntifkey="21" /label=WPRE PolyA_signal 3438..3916 /vntifkey="25" /label="hGH polyA" Misc._feature 3956..4096 /vntifkey="21" /label=R-ITR Replication_ori 4188..4494 /vntifkey="33" /label="f1 Origin" Misc._feature 5013..5870 /vntifkey="21" /label=AmpR Replication_ori 6021..6688 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTAAATAG GAATAGTGGA TTGCACAATA GCTACCCAGT 181 GCTATCAGCA TGACTCAGCA AACTTCCGCT TTCTACGAAA CCGAAACTCC CTTTCTCTTG 241 TAAGTCTGTG GTTTCCCGAA CCTATCCACT GATGACGTCA TCAGACACTG GCTACGTATG 301 CTGACGTCAG CAGACATCTG CACTACTCCA TCTGCTTTGA GCCGCGGCCG GCATTGCACA 361 ATAGAAATAG GGACAGCGCA GCATGACTCA GCATTCTACT GTATCAAGCG AAACCGAAAC 421 TGATAGCAAG TAACCAGTCT GTGGTTTTAC ACTGCCAACG ATTGATGACG TCATCAAGCC 481 CTGACACAGG ATGCTGACGT CAGCATACTG CACTTGGTGA CCATCTGCTT TTACATTATT 541 CTATGATTGC ACAATACTGC ATGCGTCTGT ACAGCATGAC TCAGCATTCA GATACGGATC 601 GCGAAACCGA AACTGTATAA CCCCTGTCTG TCTGTGGTTT CCCAATATAC TTGCTTGATG 661 ACGTCATCAG TTAACCACCG ACAGTGCTGA CGTCAGCAAT CATCTGACTT GCGCCATCTG 721 CTTACCATCC TATGATCTAT TGCACAATAA CCTAGAGACA TTCGCAGCAT GACTCAGCAA 781 TAAGTCCGGG TCGCCGAAAC CGAAACTAAG TTAGCCTGCC GCGTCTGTGG TTTGAGAGTA 841 GTGACTGTTG ATGACGTCAT CAAGAGATCG GCGAGCATGC TGACGTCAGC ACAGAATCGG 901 AGGTGGCCAT CTGCTTGCTC GAGATCTGCG ATCTGCATCT CAATTAGTCA GCAACCATAG 961 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 1021 CCCATCGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC 1081 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GGATCCGCCA 1141 CCATGGATTA TGGAGGCGCC CTGAGTGCCG TTGGGCGCGA GCTGCTATTT GTAACGAACC 1201 CAGTAGTCGT CAATGGCTCT GTACTTGTGC CTGAGGACCA GTGTTACTGC GCGGGCTGGA 1261 TTGAGTCGCG TGGCACAAAC GGTGCCCAAA CGGCGTCGAA CGTGCTGCAA TGGCTTGCTG 1321 CTGGCTTCTC CATCCTACTG CTTATGTTTT ACGCCTACCA AACATGGAAG TCAACCTGCG 1381 GCTGGGAGGA GATCTATGTG TGCGCTATCG AGATGGTCAA GGTGATTCTC GAGTTCTTCT 1441 TCGAGTTTAA GAACCCGTCC ATGCTGTATC TAGCCACAGG CCACCGCGTC CAGTGGTTGC 1501 GTTACGCCGA GTGGCTTCTC ACCTGCCCGG TCATTTGCAT TCACCTGTCA AACCTGACGG 1561 GCTTGTCCAA CGACTACAGC AGGCGCACCA TGGGTCTGCT TGTGTCTGAT ATTGGCACAA 1621 TTGTGTGGGG CGCCACTTCC GCCATGGCCA CCGGATACGT CAAGGTCATC TTCTTCTGCC 1681 TGGGTCTGTG TTATGGTGCT AACACGTTCT TTCACGCTGC CAAGGCCTAC ATCGAGGGTT 1741 ACCACACCGT GCCGAAGGGC CGGTGTCGCC AGGTGGTGAC TGGCATGGCT TGGCTCTTCT 1801 TCGTATCATG GGGTATGTTC CCCATCCTGT TCATCCTCGG CCCCGAGGGC TTCGGCGTCC 1861 TGAGCGTGTA CGGCTCCACC GTCGGCCACA CCATCATTGA CCTGATGTCG AAGAACTGCT 1921 GGGGTCTGCT CGGCCACTAC CTGCGCGTGC TGATCCACGA GCATATCCTC ATCCACGGCG 1981 ACATTCGCAA GACCACCAAA TTGAACATTG GTGGCACTGA GATTGAGGTC GAGACGCTGG 2041 TGGAGGACGA GGCCGAGGCT GGCGCGGTAC CCATGGTGAG CAAGGGCGAG GAGCTGTTCA 2101 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 2161 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA 2221 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC 2281 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC 2341 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC 2401 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG 2461 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA 2521 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC 2581 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG 2641 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA 2701 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA 2761 TCACTCTCGG CATGGACGAG CTGTACAAGT GATAAGAATT CGATATCAAG CTTATCGATA 2821 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 2881 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 2941 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 3001 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 3061 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 3121 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 3181 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 3241 CCTATGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 3301 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 3361 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGAG 3421 CGCTGCTCGA GAGATCTACG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC 3481 CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT 3541 TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA 3601 GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG 3661 TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT 3721 GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT 3781 GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC 3841 TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC 3901 TCCCTTCCCT GTCCTTCTGA TTTTGTAGGT AACCACGTGC GGACCGAGCG GCCGCAGGAA 3961 CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG 4021 CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG 4081 CGCAGCTGCC TGCAGGGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT 4141 TCACACCGCA TACGTCAAAG CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 4201 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 4261 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 4321 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 4381 TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 4441 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 4501 ACCCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 4561 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA 4621 CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC 4681 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT 4741 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC 4801 CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA 4861 TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 4921 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 4981 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 5041 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 5101 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 5161 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 5221 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 5281 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 5341 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 5401 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 5461 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 5521 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 5581 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 5641 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 5701 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 5761 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 5821 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 5881 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 5941 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 6001 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 6061 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 6121 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 6181 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 6241 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 6301 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 6361 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 6421 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 6481 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 6541 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 6601 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 6661 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGT //