LOCUS       NEWMOL       6546 bp    DNA   circular            17-APR-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|623586541|
COMMENT     VNTDBDATE|623586541|
COMMENT     LSOWNER|
COMMENT     VNTNAME|NEWMOL|
COMMENT     VNTAUTHORNAME|jisheng.chen|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "NEWMOL" 0 6546 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 1 0 0 30 100) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AflII" "ApaI" "BamHI" "BglII" "EcoRI" "EcoRV" "HindIII" 
COMMENT         "KpnI" "MluI" "NcoI" "PstI" "SacI" "SalI" "SmaI" "SpeI" "XbaI" "XhoI" 
COMMENT         "NdeI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList #0=(COligo "1241F" "TGTAGGTTTGGCAAGCTAGC" "" 0 (CStringList) 0) 
COMMENT         #1=(COligo "1563F" "CTGGGAAATCACCATAAACG" "" 0 (CStringList) 0) 
COMMENT         #2=(COligo "3'AD" "AGATGGTGCACGATGCACAG" "" 0 (CStringList) 0) 
COMMENT         #3=(COligo "3'AOX1" "GCAAATGGCATTCTGACATCC" "" 0 (CStringList) 0) 
COMMENT         #4=(COligo "3'BD" "TAAGAGTCACTTTAAAATTTGTATC" "" 0 (CStringList) 0) 
COMMENT         #5=(COligo "5'AOX1" "GACTGGTTCCAATTGACAAGC" "" 0 (CStringList) 0) 
COMMENT         #6=(COligo "a-Factor" "TACTATTGCCAGCATTGCTGC" "" 0 (CStringList) 0) 
COMMENT         #7=(COligo "BGH" "TAGAAGGCACAGTCGAGG" "" 0 (CStringList) 0) 
COMMENT         #8=(COligo "GAL4AD" "TCACACTACAATGGATG" "" 0 (CStringList) 0) 
COMMENT         #9=(COligo "GLP2" "CTTTATGTTTTTGGCGTCTTCCA" "" 0 (CStringList) 0) 
COMMENT         #10=(COligo "H1.3F" "GACGTCAATGGGAGTTTGTT" "" 0 (CStringList) 0) 
COMMENT         #11=(COligo "M13F" "GTAAAACGACGGCCAG" "" 0 (CStringList) 0) 
COMMENT         #12=(COligo "M13F(-47)" "CGCCAGGGTTTTCCCAGTCACGAC" "" 0 (CStringList) 0) 
COMMENT         #13=(COligo "M13R" "CAGGAAACAGCTATGAC" "" 0 (CStringList) 0) 
COMMENT         #14=(COligo "M13R(-48)" "AGCGGATAACAATTTCACACAGGA" "" 0 (CStringList) 0) 
COMMENT         #15=(COligo "PBV220F" "AAGAAGGGCAGCATTCAAAG" "" 0 (CStringList) 0) 
COMMENT         #16=(COligo "PBV220R" "CTGCGTTCTGATTTAATCTG" "" 0 (CStringList) 0) 
COMMENT         #17=(COligo "pEGFP-C-3'" "AAACCTCTACAAATGTGGTATG" "" 0 (CStringList) 0) 
COMMENT         #18=(COligo "pEGFP-C-5'" "CATGGTCCTGCTGGAGTTCGTG" "" 0 (CStringList) 0) 
COMMENT         #19=(COligo "pEGFP-N-3'" "CGTCGCCGTCCAGCTCGACCAG" "" 0 (CStringList) 0) 
COMMENT         #20=(COligo "pEGFP-N-5'" "TGGGAGGTCTATATAAGCAGAG" "" 0 (CStringList) 0) 
COMMENT         #21=(COligo "pGEX-3'" "CCGGGAGCTGCATGTGTCAGAGG" "" 0 (CStringList) 0) 
COMMENT         #22=(COligo "pGEX-5'" "GGGCTGGCAAGCCACGTTTGGTG" "" 0 (CStringList) 0) 
COMMENT         #23=(COligo "pRNA-CMV-F" "GTACGGTGGGAGGTCTATAT" "" 0 (CStringList) 0) 
COMMENT         #24=(COligo "RVP3" "CTAGCAAAATAGGCTGTCCC" "" 0 (CStringList) 0) 
COMMENT         #25=(COligo "S.tag" "CGAACGCCAGCACATGGAC" "" 0 (CStringList) 0) 
COMMENT         #26=(COligo "SP6" "GATTTAGGTGACACTATAG" "" 0 (CStringList) 0) 
COMMENT         #27=(COligo "T3" "ATTAACCCTCACTAAAGGGA" "" 0 (CStringList) 0) 
COMMENT         #28=(COligo "T7 promotor" "TAATACGACTCACTATAGG" "" 0 (CStringList) 0) 
COMMENT         #29=(COligo "T7 terminator" "TGCTAGTTATTGCTCAGCGG" "" 0 (CStringList) 0) 
COMMENT         #30=(COligo "U6-5'" "TACGATACAAGGCTGTTAGAGAG" "" 0 (CStringList) 0) 
COMMENT         #31=(COligo "PRM1" "AACGCAGTCAGGCACCGTGT" 
COMMENT              "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #32=(COligo "PRM2" "ATCCGTTAGCGAGGTGCCGC" 
COMMENT              "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT              0 (CStringList "PCR_PRIMER" "ANTISENSE_PRIMER") 0) 
COMMENT         #33=(COligo "SD1234S" "GATCCCCTATGGTGCACTCT" "" 0 (CStringList) 0) 
COMMENT         #34=(COligo "SiRNA5'" "CCTCCGATCGTTGTCAGAAG" "" 0 (CStringList) 0) 
COMMENT         #35=(COligo "pBac-1-seqR:" "CAACCCTTGTTTGAAACTAA" "" 0 (CStringList) 0)) 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #36=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 985 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BglII" "agatct" 2 0 2 1244 0 3286 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 283 0 2650 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2658 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 917 0 2662 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1924 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 151 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 995 0 1442 0 1496 0 1925 
COMMENT             0 0 "") 
COMMENT        #46=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 3948 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 3897 0 3908 0 
COMMENT             0 "") #50=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 1282 0 3280 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #54=(CFSignal (CObList) "Sox2+TATA box" 4 0 0 150 989 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) 
COMMENT       (CObList #55=(CMSite "1241F" "TGTAGGTTTGGCAAGCTAGC" "" 0) 
COMMENT        #56=(CMSite "1563F" "CTGGGAAATCACCATAAACG" "" 0) 
COMMENT        #57=(CMSite "3'AD" "AGATGGTGCACGATGCACAG" "" 0) 
COMMENT        #58=(CMSite "3'AOX1" "GCAAATGGCATTCTGACATCC" "" 0) 
COMMENT        #59=(CMSite "3'BD" "TAAGAGTCACTTTAAAATTTGTATC" "" 0) 
COMMENT        #60=(CMSite "5'AOX1" "GACTGGTTCCAATTGACAAGC" "" 0) 
COMMENT        #61=(CMSite "a-Factor" "TACTATTGCCAGCATTGCTGC" "" 0) 
COMMENT        #62=(CMSite "BGH" "TAGAAGGCACAGTCGAGG" "" 0) 
COMMENT        #63=(CMSite "GAL4AD" "TCACACTACAATGGATG" "" 0) 
COMMENT        #64=(CMSite "GLP2" "CTTTATGTTTTTGGCGTCTTCCA" "" 0) 
COMMENT        #65=(CMSite "H1.3F" "GACGTCAATGGGAGTTTGTT" "" 0) 
COMMENT        #66=(CMSite "M13F" "GTAAAACGACGGCCAG" "" 0) 
COMMENT        #67=(CMSite "M13F(-47)" "CGCCAGGGTTTTCCCAGTCACGAC" "" 0) 
COMMENT        #68=(CMSite "M13R" "CAGGAAACAGCTATGAC" "" 0) 
COMMENT        #69=(CMSite "M13R(-48)" "AGCGGATAACAATTTCACACAGGA" "" 0) 
COMMENT        #70=(CMSite "PBV220F" "AAGAAGGGCAGCATTCAAAG" "" 0) 
COMMENT        #71=(CMSite "PBV220R" "CTGCGTTCTGATTTAATCTG" "" 0) 
COMMENT        #72=(CMSite "pEGFP-C-3'" "AAACCTCTACAAATGTGGTATG" "" 0) 
COMMENT        #73=(CMSite "pEGFP-C-5'" "CATGGTCCTGCTGGAGTTCGTG" "" 1 2579 0 100 100 
COMMENT             100) 
COMMENT        #74=(CMSite "pEGFP-N-3'" "CGTCGCCGTCCAGCTCGACCAG" "" 1 1971 1 100 100 
COMMENT             100) #75=(CMSite "pEGFP-N-5'" "TGGGAGGTCTATATAAGCAGAG" "" 0) 
COMMENT        #76=(CMSite "pGEX-3'" "CCGGGAGCTGCATGTGTCAGAGG" "" 1 4615 0 100 100 100) 
COMMENT        #77=(CMSite "pGEX-5'" "GGGCTGGCAAGCCACGTTTGGTG" "" 0) 
COMMENT        #78=(CMSite "pRNA-CMV-F" "GTACGGTGGGAGGTCTATAT" "" 0) 
COMMENT        #79=(CMSite "RVP3" "CTAGCAAAATAGGCTGTCCC" "" 0) 
COMMENT        #80=(CMSite "S.tag" "CGAACGCCAGCACATGGAC" "" 0) 
COMMENT        #81=(CMSite "SP6" "GATTTAGGTGACACTATAG" "" 0) 
COMMENT        #82=(CMSite "T3" "ATTAACCCTCACTAAAGGGA" "" 0) 
COMMENT        #83=(CMSite "T7 promotor" "TAATACGACTCACTATAGG" "" 0) 
COMMENT        #84=(CMSite "T7 terminator" "TGCTAGTTATTGCTCAGCGG" "" 0) 
COMMENT        #85=(CMSite "U6-5'" "TACGATACAAGGCTGTTAGAGAG" "" 0) 
COMMENT        #86=(CMSite "PRM1" "AACGCAGTCAGGCACCGTGT" 
COMMENT             "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT             0) 
COMMENT        #87=(CMSite "PRM2" "ATCCGTTAGCGAGGTGCCGC" 
COMMENT             "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT             0) #88=(CMSite "SD1234S" "GATCCCCTATGGTGCACTCT" "" 0) 
COMMENT        #89=(CMSite "SiRNA5'" "CCTCCGATCGTTGTCAGAAG" "" 1 5271 1 100 100 100) 
COMMENT        #90=(CMSite "pBac-1-seqR:" "CAACCCTTGTTTGAAACTAA" "" 0)) (CObList) 
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COMMENT                          "DNA 'NEWMOL'" 1) 
COMMENT                     #95=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #96=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/17/12 10:29\311\317\316\347" 1) 
COMMENT                     #97=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/17/12 10:29\311\317\316\347" 1) 
COMMENT                     #98=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6546 bp" 1) 
COMMENT                     #99=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #100=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                           "form: Circular" 1))) "General Description") 
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COMMENT                            "Original Source Database: GenBank" 1) 
COMMENT                      #103=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "Modification Date in the Original DB: 17-APR-2012" 
COMMENT                            1))) "Standard Fields") 
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COMMENT                                      #109=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 150   End: 989 " 1))) 
COMMENT                                    "Sox2+TATA box"))) "CDS (1 total)"))) 
COMMENT                    "Feature Map") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "AflII: 0 sites") 1 5 "CTTAAG" 
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COMMENT                             (CGroupPar (CParagraph 28695728 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ApaI: 0 sites") 5 1 "GGGCCC" 
COMMENT                            "CCCGGG") 
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COMMENT                                     (CObjectList 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 985 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                                            " N1: 1244 " 1) 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3286 " 1))))) "BglII: 2 sites") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 283 " 1) 
COMMENT                                      #123=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2650 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #124=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #126=(CLinePar 
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COMMENT                                            " N1: 2658 " 1))))) "EcoRV: 1 site") 
COMMENT                            3 3 "GATATC" "CTATAG") 
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COMMENT                                            " N1: 917 " 1) 
COMMENT                                      #130=(CLinePar 
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COMMENT                                            " N2: 2662 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                     (CObjectList 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1924 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 151 " 1))))) "MluI: 1 site") 1 
COMMENT                            5 "ACGCGT" "TGCGCA") 
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COMMENT                                            " N1: 995 " 1) 
COMMENT                                      #140=(CLinePar 
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COMMENT                                            " N2: 1442 " 1) 
COMMENT                                      #141=(CLinePar 
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COMMENT                                            " N3: 1496 " 1) 
COMMENT                                      #142=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 1925 " 1))))) "NcoI: 4 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            "GTATAC") 
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COMMENT                                            " N1: 7 " 1) 
COMMENT                                      #147=(CLinePar 
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COMMENT                                            " N2: 3948 " 1))))) "PstI: 2 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                              (CObjectList)) "SacI: 0 sites") 5 1 "GAGCTC" 
COMMENT                            "CTCGAG") 
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COMMENT                            "CAGCTG") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 43 " 1) 
COMMENT                                      #153=(CLinePar 
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COMMENT                                            " N2: 54 " 1) 
COMMENT                                      #154=(CLinePar 
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COMMENT                                            " N3: 3897 " 1) 
COMMENT                                      #155=(CLinePar 
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COMMENT                                            " N4: 3908 " 1))))) "SmaI: 4 sites") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                              (CObjectList)) "SpeI: 0 sites") 1 5 "ACTAGT" 
COMMENT                            "TGATCA") 
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COMMENT                            "AGATCT") 
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COMMENT                                            " N1: 1282 " 1) 
COMMENT                                      #161=(CLinePar 
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COMMENT                                            " N2: 3280 " 1))))) "XhoI: 2 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC"))) 
COMMENT                    "Restriction/Methylation Map") 
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COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                            1) 
COMMENT                      #164=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "is calculated as an AVERAGE similarity" 1) 
COMMENT                      #165=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28654496 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #166=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #55# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #167=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #168=(COligo "1241F" 
COMMENT                                                    "TGTAGGTTTGGCAAGCTAGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TGTAGGTTTGGCAAGCTAGC" 1)))))) 
COMMENT                            "1241F (0 sites)") 
COMMENT                      #169=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28654416 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #170=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #56# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #171=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #172=(COligo "1563F" 
COMMENT                                                    "CTGGGAAATCACCATAAACG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTGGGAAATCACCATAAACG" 1)))))) 
COMMENT                            "1563F (0 sites)") 
COMMENT                      #173=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28654336 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #174=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #57# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #175=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #176=(COligo "3'AD" 
COMMENT                                                    "AGATGGTGCACGATGCACAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " AGATGGTGCACGATGCACAG" 1)))))) 
COMMENT                            "3'AD (0 sites)") 
COMMENT                      #177=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28662600 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #178=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #58# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #179=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #180=(COligo "3'AOX1" 
COMMENT                                                    "GCAAATGGCATTCTGACATCC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GCAAATGGCATTCTGACATCC" 1)))))) 
COMMENT                            "3'AOX1 (0 sites)") 
COMMENT                      #181=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28662520 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #182=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #59# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #183=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #184=(COligo "3'BD" 
COMMENT                                                    "TAAGAGTCACTTTAAAATTTGTATC" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " TAAGAGTCACTTTAAAATTTGTATC" 1)))))) 
COMMENT                            "3'BD (0 sites)") 
COMMENT                      #185=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28662120 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #186=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #60# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #187=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #188=(COligo "5'AOX1" 
COMMENT                                                    "GACTGGTTCCAATTGACAAGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GACTGGTTCCAATTGACAAGC" 1)))))) 
COMMENT                            "5'AOX1 (0 sites)") 
COMMENT                      #189=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28662040 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #190=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #61# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #191=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #192=(COligo "a-Factor" 
COMMENT                                                    "TACTATTGCCAGCATTGCTGC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TACTATTGCCAGCATTGCTGC" 1)))))) 
COMMENT                            "a-Factor (0 sites)") 
COMMENT                      #193=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28661960 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #194=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #62# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #195=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #196=(COligo "BGH" 
COMMENT                                                    "TAGAAGGCACAGTCGAGG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TAGAAGGCACAGTCGAGG" 1)))))) 
COMMENT                            "BGH (0 sites)") 
COMMENT                      #197=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28661880 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #198=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #63# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #199=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #200=(COligo "GAL4AD" 
COMMENT                                                    "TCACACTACAATGGATG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TCACACTACAATGGATG" 1)))))) 
COMMENT                            "GAL4AD (0 sites)") 
COMMENT                      #201=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28661480 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #202=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #64# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #203=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #204=(COligo "GLP2" 
COMMENT                                                    "CTTTATGTTTTTGGCGTCTTCCA" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CTTTATGTTTTTGGCGTCTTCCA" 1)))))) 
COMMENT                            "GLP2 (0 sites)") 
COMMENT                      #205=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28661400 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #206=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #65# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #207=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #208=(COligo "H1.3F" 
COMMENT                                                    "GACGTCAATGGGAGTTTGTT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GACGTCAATGGGAGTTTGTT" 1)))))) 
COMMENT                            "H1.3F (0 sites)") 
COMMENT                      #209=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938680 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #210=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #66# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #211=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #212=(COligo "M13F" 
COMMENT                                                    "GTAAAACGACGGCCAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GTAAAACGACGGCCAG" 1)))))) 
COMMENT                            "M13F (0 sites)") 
COMMENT                      #213=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938600 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #214=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #67# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #215=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #216=(COligo "M13F(-47)" 
COMMENT                                                    "CGCCAGGGTTTTCCCAGTCACGAC" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " CGCCAGGGTTTTCCCAGTCACGAC" 1)))))) 
COMMENT                            "M13F(-47) (0 sites)") 
COMMENT                      #217=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938360 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #218=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #68# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #219=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #220=(COligo "M13R" 
COMMENT                                                    "CAGGAAACAGCTATGAC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CAGGAAACAGCTATGAC" 1)))))) 
COMMENT                            "M13R (0 sites)") 
COMMENT                      #221=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938440 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #222=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #69# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #223=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #224=(COligo "M13R(-48)" 
COMMENT                                                    "AGCGGATAACAATTTCACACAGGA" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " AGCGGATAACAATTTCACACAGGA" 1)))))) 
COMMENT                            "M13R(-48) (0 sites)") 
COMMENT                      #225=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 39592432 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #226=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #90# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #227=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #228=(COligo "pBac-1-seqR:" 
COMMENT                                                    "CAACCCTTGTTTGAAACTAA" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CAACCCTTGTTTGAAACTAA" 1)))))) 
COMMENT                            "pBac-1-seqR: (0 sites)") 
COMMENT                      #229=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938520 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #230=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #70# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #231=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #232=(COligo "PBV220F" 
COMMENT                                                    "AAGAAGGGCAGCATTCAAAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " AAGAAGGGCAGCATTCAAAG" 1)))))) 
COMMENT                            "PBV220F (0 sites)") 
COMMENT                      #233=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938200 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #234=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #71# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #235=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #236=(COligo "PBV220R" 
COMMENT                                                    "CTGCGTTCTGATTTAATCTG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTGCGTTCTGATTTAATCTG" 1)))))) 
COMMENT                            "PBV220R (0 sites)") 
COMMENT                      #237=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938280 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #238=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #72# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #239=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #240=(COligo "pEGFP-C-3'" 
COMMENT                                                    "AAACCTCTACAAATGTGGTATG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " AAACCTCTACAAATGTGGTATG" 1)))))) 
COMMENT                            "pEGFP-C-3' (0 sites)") 
COMMENT                      #241=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937400 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #242=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #73# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #243=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #244=(COligo "pEGFP-C-5'" 
COMMENT                                                    "CATGGTCCTGCTGGAGTTCGTG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CATGGTCCTGCTGGAGTTCGTG" 1)) 
COMMENT                                     #245=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #73# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 2579 similarity 100.0% " 
COMMENT                                           1))))) "pEGFP-C-5' (1 site)") 
COMMENT                      #246=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937480 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #247=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #74# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #248=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #249=(COligo "pEGFP-N-3'" 
COMMENT                                                    "CGTCGCCGTCCAGCTCGACCAG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CGTCGCCGTCCAGCTCGACCAG" 1)) 
COMMENT                                     #250=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #74# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 1971 similarity 100.0% on complementary strand" 
COMMENT                                           1))))) "pEGFP-N-3' (1 site)") 
COMMENT                      #251=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937560 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #252=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #75# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #253=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #254=(COligo "pEGFP-N-5'" 
COMMENT                                                    "TGGGAGGTCTATATAAGCAGAG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " TGGGAGGTCTATATAAGCAGAG" 1)))))) 
COMMENT                            "pEGFP-N-5' (0 sites)") 
COMMENT                      #255=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937640 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #256=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #76# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #257=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #258=(COligo "pGEX-3'" 
COMMENT                                                    "CCGGGAGCTGCATGTGTCAGAGG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CCGGGAGCTGCATGTGTCAGAGG" 1)) 
COMMENT                                     #259=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #76# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 4615 similarity 100.0% " 
COMMENT                                           1))))) "pGEX-3' (1 site)") 
COMMENT                      #260=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937720 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #261=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #77# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #262=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #263=(COligo "pGEX-5'" 
COMMENT                                                    "GGGCTGGCAAGCCACGTTTGGTG" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " GGGCTGGCAAGCCACGTTTGGTG" 1)))))) 
COMMENT                            "pGEX-5' (0 sites)") 
COMMENT                      #264=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 39587688 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #265=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #86# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #266=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #267=(COligo "PRM1" 
COMMENT                                                    "AACGCAGTCAGGCACCGTGT" 
COMMENT                                                    "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " AACGCAGTCAGGCACCGTGT" 1)) 
COMMENT                                     #268=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                           1))))) "PRM1 (0 sites)") 
COMMENT                      #269=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 39610824 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #270=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #87# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #271=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #272=(COligo "PRM2" 
COMMENT                                                    "ATCCGTTAGCGAGGTGCCGC" 
COMMENT                                                    "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " ATCCGTTAGCGAGGTGCCGC" 1)) 
COMMENT                                     #273=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT                                           1))))) "PRM2 (0 sites)") 
COMMENT                      #274=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937800 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #275=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #78# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #276=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #277=(COligo "pRNA-CMV-F" 
COMMENT                                                    "GTACGGTGGGAGGTCTATAT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GTACGGTGGGAGGTCTATAT" 1)))))) 
COMMENT                            "pRNA-CMV-F (0 sites)") 
COMMENT                      #278=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937880 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #279=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #79# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #280=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #281=(COligo "RVP3" 
COMMENT                                                    "CTAGCAAAATAGGCTGTCCC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTAGCAAAATAGGCTGTCCC" 1)))))) 
COMMENT                            "RVP3 (0 sites)") 
COMMENT                      #282=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13937960 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #283=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #80# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #284=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #285=(COligo "S.tag" 
COMMENT                                                    "CGAACGCCAGCACATGGAC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CGAACGCCAGCACATGGAC" 1)))))) 
COMMENT                            "S.tag (0 sites)") 
COMMENT                      #286=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 39584784 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #287=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #88# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #288=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #289=(COligo "SD1234S" 
COMMENT                                                    "GATCCCCTATGGTGCACTCT" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GATCCCCTATGGTGCACTCT" 1)))))) 
COMMENT                            "SD1234S (0 sites)") 
COMMENT                      #290=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 39585824 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #291=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #89# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #292=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #293=(COligo "SiRNA5'" 
COMMENT                                                    "CCTCCGATCGTTGTCAGAAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CCTCCGATCGTTGTCAGAAG" 1)) 
COMMENT                                     #294=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #89# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 5271 similarity 100.0% on complementary strand" 
COMMENT                                           1))))) "SiRNA5' (1 site)") 
COMMENT                      #295=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938040 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #296=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #81# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #297=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #298=(COligo "SP6" 
COMMENT                                                    "GATTTAGGTGACACTATAG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GATTTAGGTGACACTATAG" 1)))))) 
COMMENT                            "SP6 (0 sites)") 
COMMENT                      #299=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 13938120 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #300=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #82# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #301=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #302=(COligo "T3" 
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FEATURES             Location/Qualifiers
     CDS             150..989
                     /vntifkey="4"
                     /label=Sox2+TATA\box
BASE COUNT     1414 a      1799 c      1676 g      1657 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgttcaag cctcctaccg cctttgttat 
      181 gcaaacatat caaacgccct cctttgttat gcaaaagggc tggaacgggg cctttgttat 
      241 gcaaatcgcc ctccccgatc cctttgttat gcaaatttga cgaattccca cctttgttat 
      301 gcaaacaaat ctcctaccct cctttgttat gcaaagtgag aggggctgca cctttgttat 
      361 gcaaaatgcg gcccctgaga cctttgttat gcaaaaacca tgtacgcttg cctttgttat 
      421 gcaaaccgcc tgttgcttgg cctttgttat gcaaagccac gcgattggcg cctttgttat 
      481 gcaaagctcg gttatgtaca cctttgttat gcaaagctac tttaaacttg cctttgttat 
      541 gcaaatcacg acctgaccgt cctttgttat gcaaaaacgg ttgaaatagt cctttgttat 
      601 gcaaaatgat attgaatagt cctttgttat gcaaaaattt agatgccgac cctttgttat 
      661 gcaaaggaat gggcgtgctg cctttgttat gcaaattttc gctgcgacag cctttgttat 
      721 gcaaacatgc tcgccactca cctttgttat gcaaaggtct aacaatgacc cctttgttat 
      781 gcaaactacg tggaatagat cctttgttat gcaaaccccg agtttttgaa cctttgttat 
      841 gcaaaatcag taacttcatt cctttgttat gcaaaatgtg acttaacctc cctttgttat 
      901 gcaaatatat aatggaagct tgaccggagc cgttagttgc ccgtcggacg cgatttgcga 
      961 ggcgctttat gcgcgcaccg gtcggatccg ccaccatgga ttatggaggc gccctgagtg 
     1021 ccgttgggcg cgagctgcta tttgtaacga acccagtagt cgtcaatggc tctgtacttg 
     1081 tgcctgagga ccagtgttac tgcgcgggct ggattgagtc gcgtggcaca aacggtgccc 
     1141 aaacggcgtc gaacgtgctg caatggcttg ctgctggctt ctccatccta ctgcttatgt 
     1201 tttacgccta ccaaacatgg aagtcaacct gcggctggga ggagatctat gtgtgcgcta 
     1261 tcgagatggt caaggtgatt ctcgagttct tcttcgagtt taagaacccg tccatgctgt 
     1321 atctagccac aggccaccgc gtccagtggt tgcgttacgc cgagtggctt ctcacctgcc 
     1381 cggtcatttg cattcacctg tcaaacctga cgggcttgtc caacgactac agcaggcgca 
     1441 ccatgggtct gcttgtgtct gatattggca caattgtgtg gggcgccact tccgccatgg 
     1501 ccaccggata cgtcaaggtc atcttcttct gcctgggtct gtgttatggt gctaacacgt 
     1561 tctttcacgc tgccaaggcc tacatcgagg gttaccacac cgtgccgaag ggccggtgtc 
     1621 gccaggtggt gactggcatg gcttggctct tcttcgtatc atggggtatg ttccccatcc 
     1681 tgttcatcct cggccccgag ggcttcggcg tcctgagcgt gtacggctcc accgtcggcc 
     1741 acaccatcat tgacctgatg tcgaagaact gctggggtct gctcggccac tacctgcgcg 
     1801 tgctgatcca cgagcatatc ctcatccacg gcgacattcg caagaccacc aaattgaaca 
     1861 ttggtggcac tgagattgag gtcgagacgc tggtggagga cgaggccgag gctggcgcgg 
     1921 tacccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
     1981 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
     2041 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
     2101 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 
     2161 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
     2221 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     2281 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     2341 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 
     2401 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 
     2461 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 
     2521 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 
     2581 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 
     2641 agtgataaga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg 
     2701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     2761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     2821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     2881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     2941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     3001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     3061 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg 
     3121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     3181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     3241 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca 
     3301 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac 
     3361 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa 
     3421 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag 
     3481 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc 
     3541 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt 
     3601 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac 
     3661 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc 
     3721 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta 
     3781 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc 
     3841 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 
     3901 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 
     3961 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 
     4021 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     4081 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     4141 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     4201 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 
     4261 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     4321 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 
     4381 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     4441 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 
     4501 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 
     4561 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 
     4621 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 
     4681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     4801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     4861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     4921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     4981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     5041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     5101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     5161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     5221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     5281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     5341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     5401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     5461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     5521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     5581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     5641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     5701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     5761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     5821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     5881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     5941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     6001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 
     6061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     6121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     6181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     6241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     6301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     6361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     6421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     6481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     6541 acatgt 
//
