LOCUS 157_pAAV-enh107+min_prom-Chr2d-EGFP 6179 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(618..6179,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bp circular) Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5'701bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Insertion 140..617 /origin="MluI-enh.1+min promoter element-BamHI (471bp linear) Fragment: from MluI cut at 1^2 to BamHI cut at 466^467, 465bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=Insert Promoter 145..616 /label="enhancer+min Promoter" CDS 629..1558 /vntifkey="4" /label=Chr2d CDS 1559..2281 /vntifkey="4" /label=EGFP Misc._feature 2296..2904 /vntifkey="21" /label=WPRE PolyA_signal 2924..3402 /vntifkey="25" /label="hGH polyA" Misc._feature complement(3442..3582) /vntifkey="21" /label=R-ITR Replication_ori 3674..3980 /vntifkey="33" /label="f1 Origin" Misc._feature 4499..5356 /vntifkey="21" /label=AmpR Replication_ori 5507..6174 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTACGCGT ATCCTGGGGT TTAAACAGGA CTAGGTCCAC 181 TGGGAGAAGA GGAAGTCAAA CAGGACTAGT CATCGATAGG CTAGCCAGCT TAATTAATCA 241 GATCCCTTCC AGCATGACAA TACCAGGTTT TCAAGGGCCC TAGAACTATG AGATGGTAAA 301 GTGATGCCCC CAGATGTCCT ACTCACAGCA CATGCAGCTG CTTCTCTCTC CCGGCTCTCC 361 CCTCAAGAGG CAGCTATGCA CAGGAATAGT CTAATAATCC CAAAGTCACC TAAATACTAA 421 TACAATTAAG ACTGTAACAG TTAATCCCAG ACAGCACTCT GGGCGGGGCT AGTCTCGTAC 481 ATGGAAAGGT CAGTGAAAGC CTCTATCCTC ACATGGTCCT GCTGGAGTTA GTAGAGGGTA 541 TATAATGGAA GCTCGACTTC CAGCTATCAC ATCCACTGTG TTGTTGTGAA CTGGAATCCA 601 CTATAGGCCA GGATCCGGAT CCGCCACCAT GGATTATGGA GGCGCCCTGA GTGCCGTTGG 661 GCGCGAGCTG CTATTTGTAA CGAACCCAGT AGTCGTCAAT GGCTCTGTAC TTGTGCCTGA 721 GGACCAGTGT TACTGCGCGG GCTGGATTGA GTCGCGTGGC ACAAACGGTG CCCAAACGGC 781 GTCGAACGTG CTGCAATGGC TTGCTGCTGG CTTCTCCATC CTACTGCTTA TGTTTTACGC 841 CTACCAAACA TGGAAGTCAA CCTGCGGCTG GGAGGAGATC TATGTGTGCG CTATCGAGAT 901 GGTCAAGGTG ATTCTCGAGT TCTTCTTCGA GTTTAAGAAC CCGTCCATGC TGTATCTAGC 961 CACAGGCCAC CGCGTCCAGT GGTTGCGTTA CGCCGAGTGG CTTCTCACCT GCCCGGTCAT 1021 TTGCATTCAC CTGTCAAACC TGACGGGCTT GTCCAACGAC TACAGCAGGC GCACCATGGG 1081 TCTGCTTGTG TCTGATATTG GCACAATTGT GTGGGGCGCC ACTTCCGCCA TGGCCACCGG 1141 ATACGTCAAG GTCATCTTCT TCTGCCTGGG TCTGTGTTAT GGTGCTAACA CGTTCTTTCA 1201 CGCTGCCAAG GCCTACATCG AGGGTTACCA CACCGTGCCG AAGGGCCGGT GTCGCCAGGT 1261 GGTGACTGGC ATGGCTTGGC TCTTCTTCGT ATCATGGGGT ATGTTCCCCA TCCTGTTCAT 1321 CCTCGGCCCC GAGGGCTTCG GCGTCCTGAG CGTGTACGGC TCCACCGTCG GCCACACCAT 1381 CATTGACCTG ATGTCGAAGA ACTGCTGGGG TCTGCTCGGC CACTACCTGC GCGTGCTGAT 1441 CCACGAGCAT ATCCTCATCC ACGGCGACAT TCGCAAGACC ACCAAATTGA ACATTGGTGG 1501 CACTGAGATT GAGGTCGAGA CGCTGGTGGA GGACGAGGCC GAGGCTGGCG CGGTACCCAT 1561 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG 1621 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG 1681 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT 1741 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA 1801 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT 1861 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT 1921 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA 1981 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG 2041 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA 2101 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA 2161 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT 2221 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTGATA 2281 AGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 2341 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 2401 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 2461 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 2521 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 2581 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 2641 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 2701 ATCATCGTCC TTTCCTTGGC TGCTCGCCTA TGTTGCCACC TGGATTCTGC GCGGGACGTC 2761 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 2821 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 2881 GGCCGCCTCC CCGCATCGAT ACCGAGCGCT GCTCGAGAGA TCTACGGGTG GCATCCCTGT 2941 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 3001 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 3061 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 3121 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 3181 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 3241 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 3301 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 3361 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC 3421 ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 3481 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 3541 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT 3601 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC 3661 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 3721 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 3781 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC 3841 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC 3901 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 3961 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG 4021 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 4081 GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC 4141 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG 4201 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT 4261 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG 4321 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 4381 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 4441 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 4501 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 4561 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 4621 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 4681 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 4741 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 4801 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 4861 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 4921 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 4981 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 5041 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 5101 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 5161 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 5221 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 5281 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 5341 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 5401 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 5461 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 5521 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 5581 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 5641 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 5701 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 5761 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 5821 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 5881 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 5941 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 6001 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 6061 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 6121 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGT //