LOCUS 159_pAAV-enh.112+min_prom-Chr2d-EGFP 6398 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(837..6398,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6'977bpcircular) Target vector: from BamHI cut at1415^1416 to MluI cut at 139^140, 5'701bpOrientation: original Plus strand 5': no changePlus strand 3': no change Minus strand 5': nochange Minus strand 3': no change" Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Promoter 146..835 Misc._feature 239..729 /label=enh.112 Restriction_sit join(836..>836,complement(837..>840),836^837,complement(836^837)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 298^299" /note="cut_0_on_negative_strand: 302^303" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 848..1777 /vntifkey="4" /label=Chr2d CDS 1778..2500 /vntifkey="4" /label=EGFP Misc._feature 2515..3123 /vntifkey="21" /label=WPRE PolyA_signal 3143..3621 /vntifkey="25" /label="hGH polyA" /label="hGH polyA" Misc._feature 3661..3801 /vntifkey="21" /label=R-ITR Replication_ori 3893..4199 /vntifkey="33" /label="f1 Origin" /label="f1 Origin" Misc._feature 4718..5575 /vntifkey="21" /label=AmpR Replication_ori 5726..6393 /vntifkey="33" /label="pUC ori" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTATCCTG GGGTTTAAAC AGGACTAGGT CCACTGGGAG 181 AAGAGGAAGT CAAACAGGAC TAGTCATCGA TAGGCTAGCC AGCTTAATTA ATCAGATTGT 241 CTTTCCTCCC GTCTAAGCAT GGAAGCCCTA AATGGCCTAC AATTCCTATA GCTTAGTTGC 301 AAATTTGAGA AGTTCCCCCA CTGACTCTGG TGGAGGTGGT CTATTCTGTG TCCCTGGTGA 361 AAAGTCTACC CCCTCGGGTT TGTTATGGGG CCCAGAACTT GTACAATGCC CCGTAAGCTT 421 CTTAGAGGAG GCGGGTATTA GGCAGGATTG GATCTGCGTC CCCTGACATG CCCTTCATAC 481 CCTTGAATTA GGATTCCTAA GGGAGCCGGC AGGAGGAGAA TGGGGTGTTC CTTGTGGTTC 541 CACTTCACAC CTACTCTTCG AGACCCCGGA TTCTGACAAG TTTCCCGAAC TTGGAAGACC 601 CAGATGTCAA GGTCTGAAGG TAAATGATGT GTTTAGGCAG GAGGGTTCAG CCAGGCTCAG 661 CTCTCCTCCC CTCTCGCAGG GGAAGGAGAG AGACCCTCTA GAATTCCTGT AGGAGGTGCA 721 CGTGGGCTGA TCCTCACATG GTCCTGCTGG AGTTAGTAGA GGGTATATAA TGGAAGCTCG 781 ACTTCCAGCT ATCACATCCA CTGTGTTGTT GTGAACTGGA ATCCACTATA GGCCAGGATC 841 CGCCACCATG GATTATGGAG GCGCCCTGAG TGCCGTTGGG CGCGAGCTGC TATTTGTAAC 901 GAACCCAGTA GTCGTCAATG GCTCTGTACT TGTGCCTGAG GACCAGTGTT ACTGCGCGGG 961 CTGGATTGAG TCGCGTGGCA CAAACGGTGC CCAAACGGCG TCGAACGTGC TGCAATGGCT 1021 TGCTGCTGGC TTCTCCATCC TACTGCTTAT GTTTTACGCC TACCAAACAT GGAAGTCAAC 1081 CTGCGGCTGG GAGGAGATCT ATGTGTGCGC TATCGAGATG GTCAAGGTGA TTCTCGAGTT 1141 CTTCTTCGAG TTTAAGAACC CGTCCATGCT GTATCTAGCC ACAGGCCACC GCGTCCAGTG 1201 GTTGCGTTAC GCCGAGTGGC TTCTCACCTG CCCGGTCATT TGCATTCACC TGTCAAACCT 1261 GACGGGCTTG TCCAACGACT ACAGCAGGCG CACCATGGGT CTGCTTGTGT CTGATATTGG 1321 CACAATTGTG TGGGGCGCCA CTTCCGCCAT GGCCACCGGA TACGTCAAGG TCATCTTCTT 1381 CTGCCTGGGT CTGTGTTATG GTGCTAACAC GTTCTTTCAC GCTGCCAAGG CCTACATCGA 1441 GGGTTACCAC ACCGTGCCGA AGGGCCGGTG TCGCCAGGTG GTGACTGGCA TGGCTTGGCT 1501 CTTCTTCGTA TCATGGGGTA TGTTCCCCAT CCTGTTCATC CTCGGCCCCG AGGGCTTCGG 1561 CGTCCTGAGC GTGTACGGCT CCACCGTCGG CCACACCATC ATTGACCTGA TGTCGAAGAA 1621 CTGCTGGGGT CTGCTCGGCC ACTACCTGCG CGTGCTGATC CACGAGCATA TCCTCATCCA 1681 CGGCGACATT CGCAAGACCA CCAAATTGAA CATTGGTGGC ACTGAGATTG AGGTCGAGAC 1741 GCTGGTGGAG GACGAGGCCG AGGCTGGCGC GGTACCCATG GTGAGCAAGG GCGAGGAGCT 1801 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT 1861 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT 1921 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG 1981 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 2041 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA 2101 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 2161 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG 2221 CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT 2281 CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC 2341 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT 2401 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC 2461 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTGATAA GAATTCGATA TCAAGCTTAT 2521 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 2581 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 2641 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 2701 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 2761 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 2821 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 2881 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT 2941 GCTCGCCTAT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 3001 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 3061 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA 3121 CCGAGCGCTG CTCGAGAGAT CTACGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 3181 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 3241 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 3301 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 3361 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 3421 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 3481 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 3541 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 3601 ACTGCTCCCT TCCCTGTCCT TCTGATTTTG TAGGTAACCA CGTGCGGACC GAGCGGCCGC 3661 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 3721 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 3781 GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG 3841 GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG 3901 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 3961 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4021 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4081 AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4141 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4201 ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 4261 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 4321 GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA 4381 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC 4441 CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC 4501 ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4561 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4621 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4681 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 4741 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4801 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 4861 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 4921 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 4981 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 5041 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 5101 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 5161 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5221 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5281 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5341 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5401 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5461 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5521 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5581 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 5641 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5701 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 5761 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 5821 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5881 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 5941 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 6001 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 6061 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 6121 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6181 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6241 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6301 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6361 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGT //