LOCUS       160_pAAV-synP160-Chr2d-EGFP_new      6259 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(698..6259,1..139)
                     /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6,977bp
                     circular)          Target vector: from BamHI cut at
                     1415^1416 to MluI cut at 139^140, 5,701bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
     Misc._feature   1..130
                     /vntifkey="21"
                     /label=L-ITR
     Insertion       140..697
                     /origin="synP160 (364bp linear)          Fragment: from
                     MluI cut at 1^2 to BamHI cut at 359^360, 358bp
                     Orientation: original          Plus strand 5':   no change
                     Plus strand 3':   no change          Minus strand 5':  no
                     change          Minus strand 3':  no change"
                     /label=Insert
     Restriction_sit join(<140..>143,<144..144,139^140)
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Promoter        145..696
                     /label=synP160
     Restriction_sit join(697..>697,complement(698..>701),697^698,complement(697^698))
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 359^360"
                     /note="cut_0_on_negative_strand: 363^364"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             709..1638
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1639..2361
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2376..2984
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3004..3482
                     /vntifkey="25"
                     /label="hGH polyA"
     Misc._feature   3522..3662
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3754..4060
                     /vntifkey="33"
                     /label="f1 Origin"
     Misc._feature   4579..5436
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5587..6254
                     /vntifkey="33"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTATCCTG GGGTTTAAAC AGGACTAGGT CCACTGGGAG
      181 AAGAGGAAGT CAAACAGGAC TAGTCATCGA TAGGCTAGCC AGCTTAATTA ATCAGATCTC
      241 CAGTGTTTAC CCAGGGCAAG AAGTCCCATT TTTGTTCTTT TACAAGCTGA AGACCAGAAA
      301 CACATGCGGC CCATTCCCGT CCGAGGCAGA AATGCTGAGG GATAAGTCTC AGGCTCTGAG
      361 CTGAGTTCAG GAAAAGGTCA GTGTCCCGAT AGAGCACTCA GGTAGAGTGA ATCCGCTTAT
      421 CACAGAGTGA ATCCAGCTTA GTTGTCCTTT GGAACCTCAG TTGATTGGAT AAGTCCTCCA
      481 AGTGTGAAAG AGGCATCTCC ACTCAGGCCT TGGTTCCAAT GGTCTCTCAG GGAACTGCCC
      541 TCAGCAGTGC ATAGAGCTGA GCCAGCTCTC CGGGAAGTTC AGTCGAGTTT ATCCTCACAT
      601 GGTCCTGCTG GAGTTAGTAG AGGGTATATA ATGGAAGCTC GACTTCCAGC TATCACATCC
      661 ACTGTGTTGT TGTGAACTGG AATCCACTAT AGGCCAGGAT CCGCCACCAT GGATTATGGA
      721 GGCGCCCTGA GTGCCGTTGG GCGCGAGCTG CTATTTGTAA CGAACCCAGT AGTCGTCAAT
      781 GGCTCTGTAC TTGTGCCTGA GGACCAGTGT TACTGCGCGG GCTGGATTGA GTCGCGTGGC
      841 ACAAACGGTG CCCAAACGGC GTCGAACGTG CTGCAATGGC TTGCTGCTGG CTTCTCCATC
      901 CTACTGCTTA TGTTTTACGC CTACCAAACA TGGAAGTCAA CCTGCGGCTG GGAGGAGATC
      961 TATGTGTGCG CTATCGAGAT GGTCAAGGTG ATTCTCGAGT TCTTCTTCGA GTTTAAGAAC
     1021 CCGTCCATGC TGTATCTAGC CACAGGCCAC CGCGTCCAGT GGTTGCGTTA CGCCGAGTGG
     1081 CTTCTCACCT GCCCGGTCAT TTGCATTCAC CTGTCAAACC TGACGGGCTT GTCCAACGAC
     1141 TACAGCAGGC GCACCATGGG TCTGCTTGTG TCTGATATTG GCACAATTGT GTGGGGCGCC
     1201 ACTTCCGCCA TGGCCACCGG ATACGTCAAG GTCATCTTCT TCTGCCTGGG TCTGTGTTAT
     1261 GGTGCTAACA CGTTCTTTCA CGCTGCCAAG GCCTACATCG AGGGTTACCA CACCGTGCCG
     1321 AAGGGCCGGT GTCGCCAGGT GGTGACTGGC ATGGCTTGGC TCTTCTTCGT ATCATGGGGT
     1381 ATGTTCCCCA TCCTGTTCAT CCTCGGCCCC GAGGGCTTCG GCGTCCTGAG CGTGTACGGC
     1441 TCCACCGTCG GCCACACCAT CATTGACCTG ATGTCGAAGA ACTGCTGGGG TCTGCTCGGC
     1501 CACTACCTGC GCGTGCTGAT CCACGAGCAT ATCCTCATCC ACGGCGACAT TCGCAAGACC
     1561 ACCAAATTGA ACATTGGTGG CACTGAGATT GAGGTCGAGA CGCTGGTGGA GGACGAGGCC
     1621 GAGGCTGGCG CGGTACCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
     1681 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC
     1741 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
     1801 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC
     1861 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC
     1921 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
     1981 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC
     2041 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG
     2101 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC
     2161 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
     2221 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
     2281 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
     2341 GACGAGCTGT ACAAGTGATA AGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT
     2401 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT
     2461 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC
     2521 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG
     2581 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC
     2641 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA
     2701 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT
     2761 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTA TGTTGCCACC
     2821 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT
     2881 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG
     2941 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGAGCGCT GCTCGAGAGA
     3001 TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA
     3061 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG
     3121 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA
     3181 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT
     3241 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG
     3301 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA
     3361 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC
     3421 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC
     3481 TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA
     3541 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC
     3601 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA
     3661 GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG
     3721 TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT
     3781 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC
     3841 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT
     3901 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT
     3961 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC
     4021 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC
     4081 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG
     4141 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC
     4201 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA
     4261 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG
     4321 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA
     4381 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT
     4441 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
     4501 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
     4561 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
     4621 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
     4681 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
     4741 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
     4801 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
     4861 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
     4921 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
     4981 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
     5041 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
     5101 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
     5161 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
     5221 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
     5281 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
     5341 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
     5401 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
     5461 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
     5521 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
     5581 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
     5641 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
     5701 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT
     5761 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
     5821 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
     5881 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
     5941 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
     6001 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
     6061 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
     6121 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
     6181 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
     6241 TGGCCTTTTG CTCACATGT
//
