LOCUS 178_pAAV-synP178-Chr2d-EGFP 6426 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..130 /vntifkey="21" /label=L-ITR Vector join(865..6426,1..139) /origin="P03-75-014-pAAV-EF1a-Chr2d-EGFP (6,977bp circular)Target vector: from BamHI cut at 1415^1416 to MluI cut at 139^140, 5,701bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" Insertion 140..864 /origin="178 (731bp linear)Fragment: from MluI cut at 1^2 to BamHI cut at 726^727, 725bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label=Insert Restriction_sit join(<140..>143,<144..144,139^140) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Restriction_sit join(864..>864,complement(865..>868),864^865,complement(864^865)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 720^721" /note="cut_0_on_negative_strand: 724^725" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 876..1805 /vntifkey="4" /label=Chr2d CDS 1806..2528 /vntifkey="4" /label=EGFP Misc._feature 2543..3151 /vntifkey="21" /label=WPRE PolyA_signal 3171..3649 /vntifkey="25" /label="hGH polyA" Misc._feature 3689..3829 /vntifkey="21" /label=R-ITR Replication_ori 3921..4227 /vntifkey="33" /label="f1 Origin" Misc._feature 4746..5603 /vntifkey="21" /label=AmpR Replication_ori 5754..6421 /vntifkey="33" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTATCCTG GGGTTTAAAC AGGACTAGGT CCACTGGGAG 181 AAGAGGAAGT CAAACAGGAC TAGTCATCGA TAGGCTAGCC AGCTTAATTA ATCAGATCAT 241 CCCGACCCCA GGCACAGCCC CTTCTCTGCA CCCCGCCCGT TTCAGCTGAG ACAGGGTCTC 301 CTGTAGAGTG CTCCACAGCG CCCACCCTTG GGAAGGCGGA TCTGTGATCT AGTACATGCC 361 AGGAAATGCT TCCTCATCCA CAACTCTACG CCCTTGGTTT GCCACTTCCA CGCTGTGCTG 421 AACCCTGGGG CTAACTTTCT GACCTTTCCT GGTTTACTGA GCACCAGGGA CATACATGTG 481 AACAGCTGCC ACTGGTGGCA GGCAGCTACT TAAGAAACAA ATTCAAAACC CTGTACCTCA 541 AGTCCAGTCA TATTTTAGGT GCTCATTTCA AGTGCCACAG AGCAGATTAC AGAGATCTAC 601 GGCACTTCAG AGGGGGTGAG AGGTGTAACT TTCCACGTAC TCCACACAAA AGGTCACCAC 661 TCAGACTAAG CCCGCGCAGC CCCGCTTTCC AACTCCCTAC CCTCATAATC CATCTCCTGT 721 CACGGCAATA AAATCTAGCG CTGTGGAGGA AGGATCAATC CTCACATGGT CCTGCTGGAG 781 TTAGTAGAGG GTATATAATG GAAGCTCGAC TTCCAGCTAT CACATCCACT GTGTTGTTGT 841 GAACTGGAAT CCACTATAGG CCAGGATCCG CCACCATGGA TTATGGAGGC GCCCTGAGTG 901 CCGTTGGGCG CGAGCTGCTA TTTGTAACGA ACCCAGTAGT CGTCAATGGC TCTGTACTTG 961 TGCCTGAGGA CCAGTGTTAC TGCGCGGGCT GGATTGAGTC GCGTGGCACA AACGGTGCCC 1021 AAACGGCGTC GAACGTGCTG CAATGGCTTG CTGCTGGCTT CTCCATCCTA CTGCTTATGT 1081 TTTACGCCTA CCAAACATGG AAGTCAACCT GCGGCTGGGA GGAGATCTAT GTGTGCGCTA 1141 TCGAGATGGT CAAGGTGATT CTCGAGTTCT TCTTCGAGTT TAAGAACCCG TCCATGCTGT 1201 ATCTAGCCAC AGGCCACCGC GTCCAGTGGT TGCGTTACGC CGAGTGGCTT CTCACCTGCC 1261 CGGTCATTTG CATTCACCTG TCAAACCTGA CGGGCTTGTC CAACGACTAC AGCAGGCGCA 1321 CCATGGGTCT GCTTGTGTCT GATATTGGCA CAATTGTGTG GGGCGCCACT TCCGCCATGG 1381 CCACCGGATA CGTCAAGGTC ATCTTCTTCT GCCTGGGTCT GTGTTATGGT GCTAACACGT 1441 TCTTTCACGC TGCCAAGGCC TACATCGAGG GTTACCACAC CGTGCCGAAG GGCCGGTGTC 1501 GCCAGGTGGT GACTGGCATG GCTTGGCTCT TCTTCGTATC ATGGGGTATG TTCCCCATCC 1561 TGTTCATCCT CGGCCCCGAG GGCTTCGGCG TCCTGAGCGT GTACGGCTCC ACCGTCGGCC 1621 ACACCATCAT TGACCTGATG TCGAAGAACT GCTGGGGTCT GCTCGGCCAC TACCTGCGCG 1681 TGCTGATCCA CGAGCATATC CTCATCCACG GCGACATTCG CAAGACCACC AAATTGAACA 1741 TTGGTGGCAC TGAGATTGAG GTCGAGACGC TGGTGGAGGA CGAGGCCGAG GCTGGCGCGG 1801 TACCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC 1861 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA 1921 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC 1981 CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA 2041 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA 2101 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA 2161 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG 2221 GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA 2281 AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC 2341 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA 2401 ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA 2461 TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA 2521 AGTGATAAGA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG 2581 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 2641 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 2701 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 2761 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 2821 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 2881 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 2941 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTATGT TGCCACCTGG ATTCTGCGCG 3001 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 3061 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 3121 CCCTTTGGGC CGCCTCCCCG CATCGATACC GAGCGCTGCT CGAGAGATCT ACGGGTGGCA 3181 TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC 3241 CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA 3301 TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG 3361 GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC 3421 AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT 3481 CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC 3541 CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC 3601 CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTGTA 3661 GGTAACCACG TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC 3721 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC 3781 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG 3841 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT 3901 AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 3961 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 4021 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 4081 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG 4141 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 4201 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT 4261 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 4321 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA 4381 TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC 4441 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA 4501 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG 4561 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG 4621 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 4681 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 4741 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 4801 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 4861 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 4921 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 4981 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 5041 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 5101 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 5161 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 5221 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 5281 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 5341 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 5401 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 5461 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 5521 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 5581 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 5641 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 5701 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 5761 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 5821 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 5881 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 5941 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 6001 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 6061 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 6121 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 6181 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 6241 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 6301 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 6361 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 6421 ACATGT //