LOCUS 206_pAAV-synP206-Chr2d-EGFP(fix_ITR) 6161 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..141 /vntifkey="21" /label=L-ITR Vector join(600..6161,1..150) /origin="P05-49_-pAAV-EF1a-Chr2d-EGFP(fix ITR) (6,988bp circular)Target vector: from BamHI cut at 1426^1427 to MluI cut at 150^151, 5,712bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" Insertion 151..599 /origin="206 (455bp linear)Fragment: from MluI cut at 1^2 to BamHI cut at 450^451, 449bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label=Insert Restriction_sit join(<151..>154,<155..155,150^151) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Restriction_sit join(599..>599,complement(600..>603),599^600,complement(599^600)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 450^451" /note="cut_0_on_negative_strand: 454^455" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 611..1540 /vntifkey="4" /label=Chr2d CDS 1541..2263 /vntifkey="4" /label=EGFP Misc._feature 2278..2886 /vntifkey="21" /label=WPRE PolyA_signal 2906..3384 /vntifkey="25" /label="hGH polyA" /label="hGH polyA" Misc._feature 3424..3564 /vntifkey="21" /label=R-ITR Replication_ori 3656..3962 /vntifkey="33" /label="f1 Origin" /label="f1 Origin" Misc._feature 4481..5338 /vntifkey="21" /label=AmpR Replication_ori 5489..6156 /vntifkey="33" /label="pUC ori" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC 61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA 121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATCCT GGGGTTTAAA CAGGACTAGG 181 TCCACTGGGA GAAGAGGAAG TCAAACAGGA CTAGTCATCG ATAGGCTAGC CAGCTTAATT 241 AATCAGATAC TCTAGGGCTG TGAGTGAAAG GTCGGCGAAA GTTGGAGGGC TTGGCTTGGA 301 ACTGGCAACA CTGTGCTCAT TACTGTCCGG GTAATGAGGG GACGATGCTC ATTACGGTTG 361 GTTTCTTGGC TACATGCTCA GGAATCCTCA ACTTCCTCCT GTTCAGTTCC CAGCAAGTGC 421 TGGGCATTGT GTCAGACAAG TCCCGTATAG TCCGCCATTT ATCCTGTAAG AGGCATCTGA 481 GGCACACGTC ATATCCTCAC ATGGTCCTGC TGGAGTTAGT AGAGGGTATA TAATGGAAGC 541 TCGACTTCCA GCTATCACAT CCACTGTGTT GTTGTGAACT GGAATCCACT ATAGGCCAGG 601 ATCCGCCACC ATGGATTATG GAGGCGCCCT GAGTGCCGTT GGGCGCGAGC TGCTATTTGT 661 AACGAACCCA GTAGTCGTCA ATGGCTCTGT ACTTGTGCCT GAGGACCAGT GTTACTGCGC 721 GGGCTGGATT GAGTCGCGTG GCACAAACGG TGCCCAAACG GCGTCGAACG TGCTGCAATG 781 GCTTGCTGCT GGCTTCTCCA TCCTACTGCT TATGTTTTAC GCCTACCAAA CATGGAAGTC 841 AACCTGCGGC TGGGAGGAGA TCTATGTGTG CGCTATCGAG ATGGTCAAGG TGATTCTCGA 901 GTTCTTCTTC GAGTTTAAGA ACCCGTCCAT GCTGTATCTA GCCACAGGCC ACCGCGTCCA 961 GTGGTTGCGT TACGCCGAGT GGCTTCTCAC CTGCCCGGTC ATTTGCATTC ACCTGTCAAA 1021 CCTGACGGGC TTGTCCAACG ACTACAGCAG GCGCACCATG GGTCTGCTTG TGTCTGATAT 1081 TGGCACAATT GTGTGGGGCG CCACTTCCGC CATGGCCACC GGATACGTCA AGGTCATCTT 1141 CTTCTGCCTG GGTCTGTGTT ATGGTGCTAA CACGTTCTTT CACGCTGCCA AGGCCTACAT 1201 CGAGGGTTAC CACACCGTGC CGAAGGGCCG GTGTCGCCAG GTGGTGACTG GCATGGCTTG 1261 GCTCTTCTTC GTATCATGGG GTATGTTCCC CATCCTGTTC ATCCTCGGCC CCGAGGGCTT 1321 CGGCGTCCTG AGCGTGTACG GCTCCACCGT CGGCCACACC ATCATTGACC TGATGTCGAA 1381 GAACTGCTGG GGTCTGCTCG GCCACTACCT GCGCGTGCTG ATCCACGAGC ATATCCTCAT 1441 CCACGGCGAC ATTCGCAAGA CCACCAAATT GAACATTGGT GGCACTGAGA TTGAGGTCGA 1501 GACGCTGGTG GAGGACGAGG CCGAGGCTGG CGCGGTACCC ATGGTGAGCA AGGGCGAGGA 1561 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 1621 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT 1681 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA 1741 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 1801 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 1861 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 1921 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 1981 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 2041 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 2101 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC 2161 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 2221 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGA TAAGAATTCG ATATCAAGCT 2281 TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 2341 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 2401 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 2461 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 2521 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 2581 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 2641 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG 2701 GCTGCTCGCC TATGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 2761 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 2821 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG 2881 ATACCGAGCG CTGCTCGAGA GATCTACGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 2941 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 3001 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 3061 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 3121 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 3181 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 3241 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 3301 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 3361 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC 3421 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG 3481 AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG 3541 AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 3601 GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT 3661 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 3721 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 3781 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 3841 CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 3901 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 3961 AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 4021 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 4081 AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG 4141 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC 4201 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC 4261 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 4321 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 4381 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 4441 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 4501 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 4561 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 4621 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 4681 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 4741 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 4801 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 4861 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 4921 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 4981 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 5041 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 5101 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 5161 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 5221 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 5281 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 5341 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 5401 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 5461 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 5521 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 5581 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 5641 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 5701 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 5761 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 5821 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 5881 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 5941 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 6001 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 6061 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 6121 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG T //