LOCUS 209_pAAV-synP209-Chr2d-EGFP(fix_ITR) 6220 bp DNA circular UNA 28-MAR-2011 COMMENT http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature 1..141 /vntifkey="21" /label=L-ITR Vector join(659..6220,1..150) /origin="P05-49_-pAAV-EF1a-Chr2d-EGFP(fix ITR) (6,988bp circular)Target vector: from BamHI cut at 1426^1427 to MluI cut at 150^151, 5,712bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" Insertion 151..658 /origin="209 (514bp linear)Fragment: from MluI cut at 1^2 to BamHI cut at 509^510, 508bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label=Insert Restriction_sit join(<151..>154,<155..155,150^151) /note="Name: MluI" /note="Pattern: acgcgt" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=MluI Restriction_sit join(658..>658,complement(659..>662),658^659,complement(658^659)) /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 503^504" /note="cut_0_on_negative_strand: 507^508" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI CDS 670..1599 /vntifkey="4" /label=Chr2d CDS 1600..2322 /vntifkey="4" /label=EGFP Misc._feature 2337..2945 /vntifkey="21" /label=WPRE PolyA_signal 2965..3443 /vntifkey="25" /label="hGH polyA" /label="hGH polyA" Misc._feature 3483..3623 /vntifkey="21" /label=R-ITR Replication_ori 3715..4021 /vntifkey="33" /label="f1 Origin" /label="f1 Origin" Misc._feature 4540..5397 /vntifkey="21" /label=AmpR Replication_ori 5548..6215 /vntifkey="33" /label="pUC ori" /label="pUC ori" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC 61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA 121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATCCT GGGGTTTAAA CAGGACTAGG 181 TCCACTGGGA GAAGAGGAAG TCAAACAGGA CTAGTCATCG ATAGGCTAGC CAGCTTAATT 241 AATCAGATGG CCGCTCTCCT CCCATCTGCC TCCGAAGCTC ATTTCCTGTT TCATGGCTTT 301 AGAAATAGAC CTGCCTCACT GAGCGAGAAA AGGCAAGTCA GCTCAGCTGC GAGGGCTCTG 361 CGTGTGCGCT AGCCTGGGAG ACGAGGATAT TTCGGGAGCC AGATGTCTGC GTGGGTGGAA 421 CTGCTCAAGT CTGCACAGCT CAGCGCCTAG ATACTGCCGA GTCAGCGATT CCTTTTACAT 481 CCAAGACCCC TGGTCTTTTC CCTTTGACAG GTATTTATTG ATGACTCTTA GGTGCAGACT 541 CTGAGGTAAG CATCCTCACA TGGTCCTGCT GGAGTTAGTA GAGGGTATAT AATGGAAGCT 601 CGACTTCCAG CTATCACATC CACTGTGTTG TTGTGAACTG GAATCCACTA TAGGCCAGGA 661 TCCGCCACCA TGGATTATGG AGGCGCCCTG AGTGCCGTTG GGCGCGAGCT GCTATTTGTA 721 ACGAACCCAG TAGTCGTCAA TGGCTCTGTA CTTGTGCCTG AGGACCAGTG TTACTGCGCG 781 GGCTGGATTG AGTCGCGTGG CACAAACGGT GCCCAAACGG CGTCGAACGT GCTGCAATGG 841 CTTGCTGCTG GCTTCTCCAT CCTACTGCTT ATGTTTTACG CCTACCAAAC ATGGAAGTCA 901 ACCTGCGGCT GGGAGGAGAT CTATGTGTGC GCTATCGAGA TGGTCAAGGT GATTCTCGAG 961 TTCTTCTTCG AGTTTAAGAA CCCGTCCATG CTGTATCTAG CCACAGGCCA CCGCGTCCAG 1021 TGGTTGCGTT ACGCCGAGTG GCTTCTCACC TGCCCGGTCA TTTGCATTCA CCTGTCAAAC 1081 CTGACGGGCT TGTCCAACGA CTACAGCAGG CGCACCATGG GTCTGCTTGT GTCTGATATT 1141 GGCACAATTG TGTGGGGCGC CACTTCCGCC ATGGCCACCG GATACGTCAA GGTCATCTTC 1201 TTCTGCCTGG GTCTGTGTTA TGGTGCTAAC ACGTTCTTTC ACGCTGCCAA GGCCTACATC 1261 GAGGGTTACC ACACCGTGCC GAAGGGCCGG TGTCGCCAGG TGGTGACTGG CATGGCTTGG 1321 CTCTTCTTCG TATCATGGGG TATGTTCCCC ATCCTGTTCA TCCTCGGCCC CGAGGGCTTC 1381 GGCGTCCTGA GCGTGTACGG CTCCACCGTC GGCCACACCA TCATTGACCT GATGTCGAAG 1441 AACTGCTGGG GTCTGCTCGG CCACTACCTG CGCGTGCTGA TCCACGAGCA TATCCTCATC 1501 CACGGCGACA TTCGCAAGAC CACCAAATTG AACATTGGTG GCACTGAGAT TGAGGTCGAG 1561 ACGCTGGTGG AGGACGAGGC CGAGGCTGGC GCGGTACCCA TGGTGAGCAA GGGCGAGGAG 1621 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 1681 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 1741 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 1801 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 1861 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 1921 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 1981 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 2041 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 2101 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 2161 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 2221 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 2281 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTGAT AAGAATTCGA TATCAAGCTT 2341 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 2401 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 2461 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 2521 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 2581 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 2641 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 2701 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 2761 CTGCTCGCCT ATGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 2821 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 2881 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 2941 TACCGAGCGC TGCTCGAGAG ATCTACGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC 3001 TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT 3061 GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT 3121 GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA 3181 GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA 3241 TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT 3301 AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT 3361 CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA 3421 CCACTGCTCC CTTCCCTGTC CTTCTGATTT TGTAGGTAAC CACGTGCGGA CCGAGCGGCC 3481 GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA 3541 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA 3601 GCGAGCGCGC AGCTGCCTGC AGGGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG 3661 CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA 3721 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 3781 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 3841 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 3901 AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 3961 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 4021 ACACTCAACC CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 4081 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 4141 ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 4201 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 4261 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 4321 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 4381 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 4441 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 4501 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 4561 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 4621 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 4681 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 4741 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 4801 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 4861 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 4921 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 4981 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 5041 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 5101 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 5161 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 5221 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 5281 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 5341 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 5401 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 5461 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 5521 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 5581 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 5641 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 5701 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 5761 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 5821 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 5881 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 5941 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 6001 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 6061 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 6121 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6181 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT //