LOCUS       211_pAAV-synP211-Chr2d-EGFP(fix_ITR)      6321 bp    DNA     circular UNA 28-MAR-2011
COMMENT     http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   1..141
                     /vntifkey="21"
                     /label=L-ITR
     Vector          join(760..6321,1..150)
                     /origin="P05-49_-pAAV-EF1a-Chr2d-EGFP(fix ITR) (6,988bp
                     circular)Target vector: from BamHI cut at 1426^1427 to
                     MluI cut at 150^151, 5,712bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
     Insertion       151..759
                     /origin="211 (615bp linear)Fragment: from MluI cut at 1^2
                     to BamHI cut at 610^611, 609bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
                     /label=Insert
     Restriction_sit join(<151..>154,<155..155,150^151)
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Restriction_sit join(759..>759,complement(760..>763),759^760,complement(759^760))
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 604^605"
                     /note="cut_0_on_negative_strand: 608^609"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             771..1700
                     /vntifkey="4"
                     /label=Chr2d
     CDS             1701..2423
                     /vntifkey="4"
                     /label=EGFP
     Misc._feature   2438..3046
                     /vntifkey="21"
                     /label=WPRE
     PolyA_signal    3066..3544
                     /vntifkey="25"
                     /label="hGH polyA"
                     /label="hGH polyA"
     Misc._feature   3584..3724
                     /vntifkey="21"
                     /label=R-ITR
     Replication_ori 3816..4122
                     /vntifkey="33"
                     /label="f1 Origin"
                     /label="f1 Origin"
     Misc._feature   4641..5498
                     /vntifkey="21"
                     /label=AmpR
     Replication_ori 5649..6316
                     /vntifkey="33"
                     /label="pUC ori"
                     /label="pUC ori"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTATCCT GGGGTTTAAA CAGGACTAGG
      181 TCCACTGGGA GAAGAGGAAG TCAAACAGGA CTAGTCATCG ATAGGCTAGC CAGCTTAATT
      241 AATCAGATAG GGGATTGAAG AGAACAGAGC CCAGGAACTG GACTGGAGAC AGAGAAGGTA
      301 ACAAAATGGT ATTTGTGGGT GTTTGAGAGA AGAGCAGTCG CTCCTATCCT ACACTGGCTT
      361 TGGCAGCGGC AAGCAGCCCT CAGATCTCAG CTGAGCTGAG AGGATGCTCT GAAACCAGCT
      421 TGGCCAGAGC CCTGAGGCAG GCAGGCAGAT GGATAGTCAC TTCCCGACAT GTGCAGGAGC
      481 GATAGGCAGA GTGCAGAGTC CAGGGCATAG AGGCCCAGAA CTACTGTCTG CTGCTCCACT
      541 GAGCCAGAGC TTTGCTGACC AAGTGTTCTG ACATGGCTGT ATTATGCCCT GTTGTCTGGG
      601 CACTAAATTT TATACCTCCT GAGATTCCAT ATGGCTTGGG TTGGTGCAGC AAATCCTCAC
      661 ATGGTCCTGC TGGAGTTAGT AGAGGGTATA TAATGGAAGC TCGACTTCCA GCTATCACAT
      721 CCACTGTGTT GTTGTGAACT GGAATCCACT ATAGGCCAGG ATCCGCCACC ATGGATTATG
      781 GAGGCGCCCT GAGTGCCGTT GGGCGCGAGC TGCTATTTGT AACGAACCCA GTAGTCGTCA
      841 ATGGCTCTGT ACTTGTGCCT GAGGACCAGT GTTACTGCGC GGGCTGGATT GAGTCGCGTG
      901 GCACAAACGG TGCCCAAACG GCGTCGAACG TGCTGCAATG GCTTGCTGCT GGCTTCTCCA
      961 TCCTACTGCT TATGTTTTAC GCCTACCAAA CATGGAAGTC AACCTGCGGC TGGGAGGAGA
     1021 TCTATGTGTG CGCTATCGAG ATGGTCAAGG TGATTCTCGA GTTCTTCTTC GAGTTTAAGA
     1081 ACCCGTCCAT GCTGTATCTA GCCACAGGCC ACCGCGTCCA GTGGTTGCGT TACGCCGAGT
     1141 GGCTTCTCAC CTGCCCGGTC ATTTGCATTC ACCTGTCAAA CCTGACGGGC TTGTCCAACG
     1201 ACTACAGCAG GCGCACCATG GGTCTGCTTG TGTCTGATAT TGGCACAATT GTGTGGGGCG
     1261 CCACTTCCGC CATGGCCACC GGATACGTCA AGGTCATCTT CTTCTGCCTG GGTCTGTGTT
     1321 ATGGTGCTAA CACGTTCTTT CACGCTGCCA AGGCCTACAT CGAGGGTTAC CACACCGTGC
     1381 CGAAGGGCCG GTGTCGCCAG GTGGTGACTG GCATGGCTTG GCTCTTCTTC GTATCATGGG
     1441 GTATGTTCCC CATCCTGTTC ATCCTCGGCC CCGAGGGCTT CGGCGTCCTG AGCGTGTACG
     1501 GCTCCACCGT CGGCCACACC ATCATTGACC TGATGTCGAA GAACTGCTGG GGTCTGCTCG
     1561 GCCACTACCT GCGCGTGCTG ATCCACGAGC ATATCCTCAT CCACGGCGAC ATTCGCAAGA
     1621 CCACCAAATT GAACATTGGT GGCACTGAGA TTGAGGTCGA GACGCTGGTG GAGGACGAGG
     1681 CCGAGGCTGG CGCGGTACCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
     1741 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
     1801 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC
     1861 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC
     1921 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
     1981 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
     2041 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
     2101 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
     2161 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG
     2221 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
     2281 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG
     2341 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
     2401 TGGACGAGCT GTACAAGTGA TAAGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG
     2461 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
     2521 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
     2581 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA
     2641 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG
     2701 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG
     2761 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA
     2821 ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TATGTTGCCA
     2881 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC
     2941 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC
     3001 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGAGCG CTGCTCGAGA
     3061 GATCTACGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC
     3121 CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA
     3181 GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG
     3241 GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA
     3301 ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC
     3361 CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA
     3421 GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA
     3481 CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT
     3541 CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC CGCAGGAACC CCTAGTGATG
     3601 GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC
     3661 GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG
     3721 CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
     3781 CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     3841 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     3901 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     3961 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG
     4021 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     4081 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     4141 GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     4201 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT
     4261 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA
     4321 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
     4381 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA
     4441 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
     4501 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
     4561 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     4621 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     4681 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     4741 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     4801 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     4861 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     4921 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     4981 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     5041 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     5101 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     5161 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     5221 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     5281 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     5341 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     5401 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     5461 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     5521 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     5581 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     5641 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     5701 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     5761 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     5821 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     5881 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     5941 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     6001 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     6061 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     6121 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     6181 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     6241 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     6301 GCTGGCCTTT TGCTCACATG T
//
