LOCUS       pTEI121_GFP_1-10_PCR_as_CSD1      2913 bp    DNA     circular UNA 3-MAR-2009
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(990..2913,1..323)
                     /origin="pICH41308 [CDS1] (2.845bp circular)Targetvector:
                     from BpiI cut at 921^922 to BpiI cut at
                     323^324,2.247bpOrientation: originalPlus strand 5':
                     nochangePlus strand 3':   no changeMinus strand 5':
                     nochangeMinus strand 3':  no change"
     Misc._feature   join(2390..2913,1..91)
                     /vntifkey="21"
                     /label="rep (pMB1)"
                     /label="rep (pMB1)"
     Replication_ori complement(76)
                     /vntifkey="33"
                     /label=ORI
                     /note="RNaseH cleavage point"
     Primer          184..203
                     /label=JS836
     Primer          <324..343
                     /label=TI149
     Misc._feature   324..989
                     /feature_type=Insertion
                     /origin="GFP 1-10 PCR (692bp linear)Fragment: from
                     BpiIcut at 11^12 to BpiI cut at 677^678,
                     666bpOrientation:originalPlus strand 5':   no changePlus
                     strand 3':   nochangeMinus strand 5':  no changeMinus
                     strand 3':  nochange"
                     /label=Insert
     CDS             325..988
                     /label=GFP1-10
     Primer          complement(971..>993)
                     /label=TI250
     Misc._feature   990..993
                     /vntifkey="21"
                     /label=GCTT
     Primer          complement(1107..1124)
                     /label=JS837
     CDS             1220..2230
                     /vntifkey="4"
                     /label="Sm/Sp no DraIII"
                     /label="Sm/Sp no DraIII"
     Mutation        2197
                     /vntifkey="62"
                     /label="G to A"
                     /label="G to A"
ORIGIN
        1 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
       61 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
      121 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
      181 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
      241 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
      301 TCATTAATCA CTCTGTGGTC TCAAATGTCC AAAGGAGAAG AACTGTTTAC CGGTGTTGTG
      361 CCAATTTTGG TTGAACTCGA TGGTGATGTC AACGGACATA AGTTCTCAGT GAGAGGCGAA
      421 GGAGAAGGTG ACGCCACCAT TGGAAAATTG ACTCTTAAAT TCATCTGTAC TACTGGTAAA
      481 CTTCCTGTAC CATGGCCGAC TCTCGTAACA ACGCTTACGT ACGGAGTTCA GTGCTTTTCG
      541 AGATACCCAG ACCATATGAA AAGACATGAC TTTTTTAAGT CGGCTATGCC TGAAGGTTAC
      601 GTGCAAGAAA GAACAATTTC GTTCAAAGAT GATGGAAAAT ATAAAACTAG AGCAGTTGTT
      661 AAATTTGAAG GAGATACTTT GGTTAACCGC ATTGAACTGA AAGGAACAGA TTTTAAAGAA
      721 GATGGTAATA TTCTTGGACA CAAACTCGAA TACAATTTTA ATAGTCATAA CGTATACATC
      781 ACTGCTGATA AGCAAAAGAA CGGAATTAAA GCGAATTTCA CAGTACGCCA TAATGTAGAA
      841 GATGGCAGTG TTCAACTTGC CGACCATTAC CAACAAAACA CCCCTATTGG AGACGGTCCG
      901 GTACTTCTTC CTGATAATCA CTACCTCTCA ACACAAACAG TCCTGAGCAA AGATCCAAAT
      961 GAAAAAGGAA CAGGTGGCGG CGGAAGTTAG CTTTGAGACC ACGAAGTGGC TCTTCAGTGG
     1021 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
     1081 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
     1141 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
     1201 ATATTGAAAA AGGAAGAGTA TGCGCTCACG CAACTGGTCC AGAACCTTGA CCGAACGCAG
     1261 CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT GACTGTTTTT TTGGGGTACA
     1321 GTCTATGCCT CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT GTTTGATGTT
     1381 ATGGAGCAGC AACGATGTTA CGCAGCAGGG CAGTCGCCCT AAAACAAAGT TAAACATCAT
     1441 GAGGGAAGCG GTGATCGCCG AAGTATCGAC TCAACTATCA GAGGTAGTTG GCGTCATCGA
     1501 GCGCCATCTC GAACCGACGT TGCTGGCCGT ACATTTGTAC GGCTCCGCAG TGGATGGCGG
     1561 CCTGAAGCCA CACAGCGATA TTGATTTGCT GGTTACGGTG ACCGTAAGGC TTGATGAAAC
     1621 AACGCGGCGA GCTTTGATCA ACGACCTTTT GGAAACTTCG GCTTCCCCTG GAGAGAGCGA
     1681 GATTCTCCGC GCTGTAGAAG TCACCATTGT TGTGCACGAC GACATCATTC CGTGGCGTTA
     1741 TCCAGCTAAG CGCGAACTGC AATTTGGAGA ATGGCAGCGC AATGACATTC TTGCAGGTAT
     1801 CTTCGAGCCA GCCACGATCG ACATTGATCT GGCTATCTTG CTGACAAAAG CAAGAGAACA
     1861 TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA GGAACTCTTT GATCCGGTTC CTGAACAGGA
     1921 TCTATTTGAG GCGCTAAATG AAACCTTAAC GCTATGGAAC TCGCCGCCCG ACTGGGCTGG
     1981 CGATGAGCGA AATGTAGTGC TTACGTTGTC CCGCATTTGG TACAGCGCAG TAACCGGCAA
     2041 AATCGCGCCG AAGGATGTCG CTGCCGACTG GGCAATGGAG CGCCTGCCGG CCCAGTATCA
     2101 GCCCGTCATA CTTGAAGCTA GACAGGCTTA TCTTGGACAA GAAGAAGATC GCTTGGCCTC
     2161 GCGCGCAGAT CAGTTGGAAG AATTTGTCCA TTACGTAAAA GGCGAGATCA CCAAGGTAGT
     2221 CGGCAAATAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     2281 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     2341 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     2401 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     2461 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     2521 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     2581 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     2641 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     2701 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     2761 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     2821 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     2881 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAG
//
