LOCUS       pTEI123_GFP_11x3_PCR_as_CT      2449 bp    DNA     circular UNA 23-OCT-2012
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(553..2449,1..350)
                     /origin="pAGM1301 [CT] (2.845bp circular)Target
                     vector:from BpiI cut at 948^949 to BpiI cut at
                     350^351,2.247bpOrientation: originalPlus strand 5':
                     nochangePlus strand 3':   no changeMinus strand 5':
                     nochangeMinus strand 3':  no change"
     Misc._feature   join(1953..2449,1..118)
                     /vntifkey="21"
                     /label="rep (pMB1)"
                     /label="rep (pMB1)"
     Replication_ori complement(103)
                     /vntifkey="33"
                     /label=ORI
                     /note="RNaseH cleavage point"
     Primer          211..230
                     /label=JS836
     Primer          <351..374
                     /label=TI253
     Misc._feature   351..552
                     /feature_type=Insertion
                     /origin="11x3 PCR (228bp linear)Fragment: from BpiI cutat
                     11^12 to BpiI cut at 213^214,
                     202bpOrientation:originalPlus strand 5':   no changePlus
                     strand 3':   nochangeMinus strand 5':  no changeMinus
                     strand 3':  nochange"
                     /label=Insert
     CDS             352..369
                     /label=linker
     CDS             370..552
                     /label=3xGFP11
     Primer          complement(535..>556)
                     /label=TI254
     Misc._feature   553..556
                     /vntifkey="21"
                     /label=GCTT
     Primer          complement(670..687)
                     /label=JS837
     CDS             783..1793
                     /vntifkey="4"
                     /label="Sm/Sp no DraIII"
                     /label="Sm/Sp no DraIII"
     Mutation        1760
                     /vntifkey="62"
                     /label="G to A"
                     /label="G to A"
ORIGIN
        1 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
       61 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
      121 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
      181 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG
      241 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA
      301 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATCACTC TGTGGTCTCA TTCGATGGGC
      361 GGCAAATTCA TGCGTGACCA CATGGTCCTT CATGAGTATG TAAATGCTGC TGGGATTACA
      421 GGTGGCTCTG GAGGTAGAGA TCATATGGTT CTCCACGAAT ACGTTAACGC CGCAGGCATC
      481 ACTGGCGGTA GTGGAGGACG CGACCATATG GTACTACATG AATATGTCAA TGCAGCCGGA
      541 ATAACCGGAT AAGCTTTGAG ACCACGAAGT GGCTCTTCAG TGGACGAAAG GGCCTCGTGA
      601 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
      661 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
      721 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
      781 GTATGCGCTC ACGCAACTGG TCCAGAACCT TGACCGAACG CAGCGGTGGT AACGGCGCAG
      841 TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGGGGT ACAGTCTATG CCTCGGGCAT
      901 CCAAGCAGCA AGCGCGTTAC GCCGTGGGTC GATGTTTGAT GTTATGGAGC AGCAACGATG
      961 TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAAACAT CATGAGGGAA GCGGTGATCG
     1021 CCGAAGTATC GACTCAACTA TCAGAGGTAG TTGGCGTCAT CGAGCGCCAT CTCGAACCGA
     1081 CGTTGCTGGC CGTACATTTG TACGGCTCCG CAGTGGATGG CGGCCTGAAG CCACACAGCG
     1141 ATATTGATTT GCTGGTTACG GTGACCGTAA GGCTTGATGA AACAACGCGG CGAGCTTTGA
     1201 TCAACGACCT TTTGGAAACT TCGGCTTCCC CTGGAGAGAG CGAGATTCTC CGCGCTGTAG
     1261 AAGTCACCAT TGTTGTGCAC GACGACATCA TTCCGTGGCG TTATCCAGCT AAGCGCGAAC
     1321 TGCAATTTGG AGAATGGCAG CGCAATGACA TTCTTGCAGG TATCTTCGAG CCAGCCACGA
     1381 TCGACATTGA TCTGGCTATC TTGCTGACAA AAGCAAGAGA ACATAGCGTT GCCTTGGTAG
     1441 GTCCAGCGGC GGAGGAACTC TTTGATCCGG TTCCTGAACA GGATCTATTT GAGGCGCTAA
     1501 ATGAAACCTT AACGCTATGG AACTCGCCGC CCGACTGGGC TGGCGATGAG CGAAATGTAG
     1561 TGCTTACGTT GTCCCGCATT TGGTACAGCG CAGTAACCGG CAAAATCGCG CCGAAGGATG
     1621 TCGCTGCCGA CTGGGCAATG GAGCGCCTGC CGGCCCAGTA TCAGCCCGTC ATACTTGAAG
     1681 CTAGACAGGC TTATCTTGGA CAAGAAGAAG ATCGCTTGGC CTCGCGCGCA GATCAGTTGG
     1741 AAGAATTTGT CCATTACGTA AAAGGCGAGA TCACCAAGGT AGTCGGCAAA TAACTGTCAG
     1801 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     1861 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     1921 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     1981 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     2041 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     2101 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     2161 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     2221 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     2281 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     2341 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     2401 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCC
//
