LOCUS       pTEI127_1-2f_35S_STMTR_GFP1-10_tocs      7487 bp    DNA     circular UNA 3-SEP-2009
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(7455..7487,1..4339)
                     /origin="pICH47742 1-2f (4.968bp circular)Target
                     vector:from BsaI cut at 4935^4936 to BsaI cut at
                     4339^4340,4.372bpOrientation: originalPlus strand 5':
                     nochangePlus strand 3':   no changeMinus strand 5':
                     nochangeMinus strand 3':  no change"
     Misc._feature   complement(23..170)
                     /vntifkey="21"
                     /label=TDNA-LB
                     /note="shows similarity to T-DNA left border: GenBank
                     Accession Number J01825"
     Replication_ori 200..817
                     /standard_name="oriV"
                     /vntifkey="33"
                     /label=oriV
                     /note="shows similarity to GenBank Accession Number
                     M20134"
     CDS             1015..1872
                     /vntifkey="4"
                     /label="AP r"
                     /label="AP r"
     Misc._feature   complement(1880..2669)
                     /vntifkey="21"
                     /label="pUC ori"
                     /label="pUC ori"
     Replication_ori complement(2634)
                     /vntifkey="33"
                     /label=ORI
                     /note="RNaseH cleavage point"
     Misc._feature   2680..4161
                     /vntifkey="21"
                     /label="RK2 trfa (no Esp3I)"
                     /label="RK2 trfa (no Esp3I)"
     Misc._feature   4185..4312
                     /vntifkey="21"
                     /label="RB short"
                     /label="RB short"
     Misc._feature   4340..5057
                     /feature_type=Insertion
                     /origin="D12_pICH41432 tocs (2.965bp
                     circular)Fragment:from BsaI cut at 2231^2232 to BsaI cut
                     at 2949^2950,718bpOrientation: reverse complementedPlus
                     strand 5':no changePlus strand 3':   no changeMinus
                     strand 5':  nochangeMinus strand 3':  no change"
                     /label=Insert
     Terminator      complement(4344..5057)
                     /vntifkey="43"
                     /label=ocst
     Primer          <5058..5080
                     /label=TI250
     CDS             complement(5058..5061)
                     /vntifkey="4"
                     /label=GCTT
     Misc._feature   5058..5723
                     /feature_type=Insertion
                     /origin="pTEI121_GFP 1-10 PCR as CSD1
                     (2.913bpcircular)Fragment: from BsaI cut at 323^324 to
                     BsaI cutat 989^990, 666bpOrientation: reverse
                     complementedPlusstrand 5':   no changePlus strand 3':
                     no changeMinusstrand 5':  no changeMinus strand 3':  no
                     change"
                     /label=Insert
     Misc._feature   complement(5062..5727)
                     /feature_type=Insertion
                     /origin="GFP 1-10 PCR (692bp linear)Fragment: from
                     BpiIcut at 11^12 to BpiI cut at 677^678,
                     666bpOrientation:originalPlus strand 5':   no changePlus
                     strand 3':   nochangeMinus strand 5':  no changeMinus
                     strand 3':  nochange"
                     /label=Insert
     CDS             complement(5063..5726)
                     /label=GFP1-10
     Primer          complement(5708..>5727)
                     /label=TI149
     Primer          <5724..5749
                     /label=TI248
     Misc._feature   5724..6027
                     /feature_type=Insertion
                     /origin="pTEI120_STMTR 1-100 PCR as NT1
                     (2.551bpcircular)Fragment: from BsaI cut at 378^379 to
                     BsaI cutat 682^683, 304bpOrientation: reverse
                     complementedPlusstrand 5':   no changePlus strand 3':
                     no changeMinusstrand 5':  no changeMinus strand 3':  no
                     change"
                     /label=Insert
     Misc._feature   complement(5728..6031)
                     /feature_type=Insertion
                     /origin="STMTR 1-100 PCR (330bp linear)Fragment: fromBpiI
                     cut at 11^12 to BpiI cut at 315^316,304bpOrientation:
                     originalPlus strand 5':   no changePlusstrand 3':   no
                     changeMinus strand 5':  no changeMinusstrand 3':  no
                     change"
                     /label=Insert
     CDS             complement(5730..6029)
                     /label=STMTR1-100
     Primer          complement(6010..>6031)
                     /label=TI247
     Primer          <6028..6049
                     /label=1353
     Misc._feature   6028..7454
                     /feature_type=Insertion
                     /origin="pJOG640_p35S-long [Pro+5Uf]
                     (3.674bpcircular)Fragment: from BsaI cut at 548^549 to
                     BsaI cutat 1975^1976, 1.427bpOrientation:
                     reversecomplementedPlus strand 5':   no changePlus strand
                     3':no changeMinus strand 5':  no changeMinus strand 3':
                     nochange"
                     /label=Insert
     Misc._feature   complement(6032..7458)
                     /feature_type=Insertion
                     /origin="H2_pICH51266_35Slong selection
                     (1.453bplinear)Fragment: from BpiI cut at 11^12 to BpiI
                     cut at1438^1439, 1.427bpOrientation: originalPlus strand
                     5':no changePlus strand 3':   no changeMinus strand 5':
                     nochangeMinus strand 3':  no change"
                     /label=Insert
     5'_UTR          complement(6039..6100)
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega leader"
                     /label="Omega leader"
     Promoter        complement(6117..7454)
                     /vntifkey="29"
                     /label="35S promoter (no BsaI and BpiI)"
                     /label="CaMV 35S promoter"
                     /label="35S promoter (no BsaI and BpiI)"
     Primer          complement(7440..>7458)
                     /label=1352
     Misc._feature   complement(7455..>7458)
                     /label="GGAG 4bp overhang"
                     /label="GGAG 4bp overhang"
ORIGIN
        1 GTGGGGCCCA CTGCATCCAC CCCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG
       61 TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC
      121 CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCAGATCAGG
      181 ATCTCCTTTG CGACGCTCAC CGGGCTGGTT GCCCTCGCCG CTGGGCTGGC GGCCGTCTAT
      241 GGCCCTGCAA ACGCGCCAGA AACGCCGTCG AAGCCGTGTG CGAGACACCG CGGCCGCCGG
      301 CGTTGTGGAT ACCTCGCGGA AAACTTGGCC CTCACTGACA GATGAGGGGC GGACGTTGAC
      361 ACTTGAGGGG CCGACTCACC CGGCGCGGCG TTGACAGATG AGGGGCAGGC TCGATTTCGG
      421 CCGGCGACGT GGAGCTGGCC AGCCTCGCAA ATCGGCGAAA ACGCCTGATT TTACGCGAGT
      481 TTCCCACAGA TGATGTGGAC AAGCCTGGGG ATAAGTGCCC TGCGGTATTG ACACTTGAGG
      541 GGCGCGACTA CTGACAGATG AGGGGCGCGA TCCTTGACAC TTGAGGGGCA GAGTGCTGAC
      601 AGATGAGGGG CGCACCTATT GACATTTGAG GGGCTGTCCA CAGGCAGAAA ATCCAGCATT
      661 TGCAAGGGTT TCCGCCCGTT TTTCGGCCAC CGCTAACCTG TCTTTTAACC TGCTTTTAAA
      721 CCAATATTTA TAAACCTTGT TTTTAACCAG GGCTGCGCCC TGTGCGCGTG ACCGCGCACG
      781 CCGAAGGGGG GTGCCCCCCC TTCTCGAACC CTCCCGGCCC GCTAACGCGG GCCTCCCATC
      841 CCCCCAGGGG CTGCGCCCCT CGGCCGCGAA CGGCCTCACC CCAAAAATGG CAGCGCTGGC
      901 CAATTCGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG
      961 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
     1021 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT
     1081 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG
     1141 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA
     1201 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT
     1261 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
     1321 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT
     1381 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA
     1441 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT
     1501 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA
     1561 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
     1621 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC
     1681 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG TTCTCGCGGT
     1741 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG
     1801 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG
     1861 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA
     1921 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA
     1981 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA
     2041 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG
     2101 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT
     2161 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC
     2221 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG
     2281 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG
     2341 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA
     2401 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC
     2461 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
     2521 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC
     2581 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC
     2641 AGCAACGCGG CCTTTTTACG GTTCCTGGCA GATCCTAGAT GTGGCGCAAC GATGCCGGCG
     2701 ACAAGCAGGA GCGCACCGAC TTCTTCCGCA TCAAGTGTTT TGGCTCTCAG GCCGAGGCCC
     2761 ACGGCAAGTA TTTGGGCAAG GGGTCGCTGG TATTCGTGCA GGGCAAGATT CGGAATACCA
     2821 AGTACGAGAA GGACGGCCAG ACGGTCTACG GGACCGACTT CATTGCCGAT AAGGTGGATT
     2881 ATCTGGACAC CAAGGCACCA GGCGGGTCAA ATCAGGAATA AGGGCACATT GCCCCGGCGT
     2941 GAGTCGGGGC AATCCCGCAA GGAGGGTGAA TGAATCGGAC GTTTGACCGG AAGGCATACA
     3001 GGCAAGAACT GATCGACGCG GGGTTTTCCG CCGAGGATGC CGAAACCATC GCAAGCCGCA
     3061 CCGTCATGCG TGCGCCCCGC GAAACCTTCC AGTCCGTCGG CTCGATGGTC CAGCAAGCTA
     3121 CGGCCAAGAT CGAGCGCGAC AGCGTGCAAC TGGCTCCCCC TGCCCTGCCC GCGCCATCGG
     3181 CCGCCGTGGA GCGTTCGCGT CGTCTTGAAC AGGAGGCGGC AGGTTTGGCG AAGTCGATGA
     3241 CCATCGACAC GCGAGGAACT ATGACGACCA AGAAGCGAAA AACCGCCGGC GAGGACCTGG
     3301 CAAAACAGGT CAGCGAGGCC AAGCAGGCCG CGTTGCTGAA ACACACGAAG CAGCAGATCA
     3361 AGGAAATGCA GCTTTCCTTG TTCGATATTG CGCCGTGGCC GGACACGATG CGAGCGATGC
     3421 CAAACGACAC GGCCCGCTCT GCCCTGTTCA CCACGCGCAA CAAGAAAATC CCGCGCGAGG
     3481 CGCTGCAAAA CAAGGTCATT TTCCACGTCA ACAAGGACGT GAAGATCACC TACACCGGCG
     3541 TCGAGCTGCG GGCCGACGAT GACGAACTGG TGTGGCAGCA GGTGTTGGAG TACGCGAAGC
     3601 GCACCCCTAT CGGCGAGCCG ATCACCTTCA CGTTCTACGA GCTTTGCCAG GACCTGGGCT
     3661 GGTCGATCAA TGGCCGGTAT TACACGAAGG CCGAGGAATG CCTGTCGCGC CTACAGGCGA
     3721 CGGCGATGGG CTTCACGTCC GACCGCGTTG GGCACCTGGA ATCGGTGTCG CTGCTGCACC
     3781 GCTTCCGCGT CCTGGACCGT GGCAAGAAAA CGTCCCGTTG CCAGGTCCTG ATCGACGAGG
     3841 AAATCGTCGT GCTGTTTGCT GGCGACCACT ACACGAAATT CATATGGGAG AAGTACCGCA
     3901 AGCTGTCGCC GACGGCCCGA CGGATGTTCG ACTATTTCAG CTCGCACCGG GAGCCGTACC
     3961 CGCTCAAGCT GGAAACCTTC CGCCTCATGT GCGGATCGGA TTCCACCCGC GTGAAGAAGT
     4021 GGCGCGAGCA GGTCGGCGAA GCCTGCGAAG AGTTGCGAGG CAGCGGCCTG GTGGAACACG
     4081 CCTGGGTCAA TGATGACCTG GTGCATTGCA AACGCTAGGG CCTTGTGGGG TCAGTTCCGG
     4141 CTGGGGGTTC AGCAGCCAGC GCCTGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA
     4201 ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC
     4261 TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACCACT
     4321 TCGTGCAGAA GACAATAGTA GCGGTCCTGC TGAGCCTCGA CATGTTGTCG CAAAATTCGC
     4381 CCTGGACCCG CCCAACGATT TGTCGTCACT GTCAAGGTTT GACCTGCACT TCATTTGGGG
     4441 CCCACATACA CCAAAAAAAT GCTGCATAAT TCTCGGGGCA GCAAGTCGGT TACCCGGCCG
     4501 CCGTGCTGGA CCGGGTTGAA TGGTGCCCGT AACTTTCGGT AGAGCGGACG GCCAATACTC
     4561 AACTTCAAGG AATCTCACCC ATGCGCGCCG GCGGGGAACC GGAGTTCCCT TCAGTGAGCG
     4621 TTATTAGTTC GCCGCTCGGT GTGTCGTAGA TACTAGCCCC TGGGGCACTT TTGAAATTTG
     4681 AATAAGATTT ATGTAATCAG TCTTTTAGGT TTGACCGGTT CTGCCGCTTT TTTTAAAATT
     4741 GGATTTGTAA TAATAAAACG CAATTGTTTG TTATTGTGGC GCTCTATCAT AGATGTCGCT
     4801 ATAAACCTAT TCAGCACAAT ATATTGTTTT CATTTTAATA TTGTACATAT AAGTAGTAGG
     4861 GTACAATCAG TAAATTGAAC GGAGAATATT ATTCATAAAA ATACGATAGT AACGGGTGAT
     4921 ATATTCATTA GAATGAACCG AAACCGGCGG TAAGGATCTG AGCTACACAT GCTCAGGTTT
     4981 TTTACAACGT GCACAACAGA ATTGAAAGCA AATATCATGC GATCATAGGC TTCTCGCATA
     5041 TCTCATTAAA GCAGGACAAG CTAACTTCCG CCGCCACCTG TTCCTTTTTC ATTTGGATCT
     5101 TTGCTCAGGA CTGTTTGTGT TGAGAGGTAG TGATTATCAG GAAGAAGTAC CGGACCGTCT
     5161 CCAATAGGGG TGTTTTGTTG GTAATGGTCG GCAAGTTGAA CACTGCCATC TTCTACATTA
     5221 TGGCGTACTG TGAAATTCGC TTTAATTCCG TTCTTTTGCT TATCAGCAGT GATGTATACG
     5281 TTATGACTAT TAAAATTGTA TTCGAGTTTG TGTCCAAGAA TATTACCATC TTCTTTAAAA
     5341 TCTGTTCCTT TCAGTTCAAT GCGGTTAACC AAAGTATCTC CTTCAAATTT AACAACTGCT
     5401 CTAGTTTTAT ATTTTCCATC ATCTTTGAAC GAAATTGTTC TTTCTTGCAC GTAACCTTCA
     5461 GGCATAGCCG ACTTAAAAAA GTCATGTCTT TTCATATGGT CTGGGTATCT CGAAAAGCAC
     5521 TGAACTCCGT ACGTAAGCGT TGTTACGAGA GTCGGCCATG GTACAGGAAG TTTACCAGTA
     5581 GTACAGATGA ATTTAAGAGT CAATTTTCCA ATGGTGGCGT CACCTTCTCC TTCGCCTCTC
     5641 ACTGAGAACT TATGTCCGTT GACATCACCA TCGAGTTCAA CCAAAATTGG CACAACACCG
     5701 GTAAACAGTT CTTCTCCTTT GGACATTGCG CTAGGATCTC CAGGTGGATA ACGCTCATGG
     5761 TTGGAGTCAT CATATACAAT TTTTGAACTG GGATTCTTAA TAGCAGCCAC TGTGGCTGGA
     5821 AGATCTGAGA CTAAACCCAA CTGATGTGCG GGAGAAACCG AATTAGATGA GAAACCTCTG
     5881 ATTTGATCGA GAAACGAAGA GGTGAGTGAC GGAGAAGGAG ATCTTGCCGG AGACGAATCA
     5941 TCGGTAACGG TGAAAGAGCT TCTTGTAAAG AAGGTAGATG AAGATCTAGC GGCTGAAGAA
     6001 GAAAGCCTTC TACCTGCTAC TCGAAGCATG GTATCGATAA TTGTAAATGT AATTGTAATG
     6061 TTGTTTGTTG TTTGTTGTTG TTGGTAATTG TTGTAAAAAT GAGCTCTTAT ACTCGAGCGT
     6121 GTCCTCTCCA AATGAAATGA ACTTCCTTAT ATAGAGGAAG GGTCTTGCGA AGGATAGTGG
     6181 GATTGTGCGT CATCCCTTAC GTCAGTGGAG ATGTCACATC AATCCACTTG CTTTGTAGAC
     6241 GTGGTTGGAA CCTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA
     6301 CCACTGTCGG CAGAGAGATC TTGAATGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG
     6361 TAGGAGCCAC CTTCCTTTTC TACTGTCCTT TCGATGAAGT GACAGATAGC TGGGCAATGG
     6421 AATCCGAGGA GGTTTCCCGA AATTATCCTT TGTTGAAAAG TCTCAATAGC CCTTTGATCT
     6481 TCTGAGACTG TATCTTTGAC ATTTTTGGAG TAGACCAGAG TGTCGTGCTC CACCATGTTG
     6541 ACGAAGATTT TCTTCTTGTC ATTGAGTCGT AAAAGACTCT GTATGAACTG TTCGCCAGTC
     6601 TTGACGGCGA GTTCTGTTAG ATCCTCGATT TGAATCTTAG ACTCCATGCA TGGCCTTAGA
     6661 TTCAGTAGGA ACTACCTTTT TAGAGACTCC AATCTCTATT ACTTGCCTTG GTTTATGAAG
     6721 CAAGCCTTGA ATCGTCCATA CTGGAATAGT ACTTCTGATC TTGAGAAATA TGTCTTTCTC
     6781 TGTGTTCTTG ATGCAATTAG TCCTGAATCT TTTGACTGCA TCTTTAACCT TCTTGGGAAG
     6841 GTATTTGATC TCCTGGAGAT TGTTACTCGG GTAGATCGTC TTGATGAGAC TTGCTGCGTA
     6901 GGCCTCTCTA ACCATCTGTG GGTCAGCATT CTTTCTGAAA TTGAAGAGGC TAACCTTCTC
     6961 ATTATCAGTG GTGAACATAG TGTCGTCACC TTCACCTTCG AACTTCCTTC CTAGATCGTA
     7021 AAGATAGAGG AAATCGTCCA TTGTAATCTC CGGGGCAAAG GAGATCTCTT TTGGGGCTGG
     7081 ATCACTGCTG GGCCTTTTGG TTCCTAGCGT GAGCCAGTGG GCTTTTTGCT TTGGTGGGCT
     7141 TGTTAGGGCC TTAGCAAAGC TCTTGGGCTT GAGTTGAGCT TCTCCTTTGG GGATGAAGTT
     7201 CAACCTGTCT GTTTGCTGAC TTGTTGTGTA CGCGTCAGCT GCTGCTCTTG CCTCTGTAAT
     7261 AGTGGCAAAT TTCTTGTGTG CAACTCCGGG AACGCCGTTT GTTGCCGCCT TTGTACAACC
     7321 CCAGTCATCG TATATACCGG CATGTGGACC GTTATACACA ACGTAGTAGT TGATATGAGG
     7381 GTGTTGAATA CCCGATTCTG CTCTGAGAGG AGCAACTGTG CTGTTAAGCT CAGATTTTTG
     7441 TGGGATTGGA ATTCCTCCAA GCTTGAATTC TTGCTTGTCT TCACAGA
//
