LOCUS Exported 2787 bp ds-DNA circular SYN 26-MAR-2019 DEFINITION Sourced from Addgene. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2787) AUTHORS Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, Keasling JD. TITLE BglBricks: A flexible standard for biological part assembly. JOURNAL J Biol Eng 2010;4:1. PUBMED 20205762 REFERENCE 2 (bases 1 to 2787) AUTHORS Sandra TITLE Direct Submission JOURNAL Exported Tuesday, Mar 26, 2019 from SnapGene 4.3.4 https://www.snapgene.com COMMENT Kanamycin resistance Ptrc inducible promoter p15A origin of replication RFP tagged FEATURES Location/Qualifiers source 1..2787 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 7..41 /label=J23150 primer_bind 35..71 /label=BbJ231xxF CDS 87..764 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 797..868 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 884..911 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1073..1618) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." terminator 1732..1826 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1857..2651) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind 2695..2714 /label=pBbkpromseq primer_bind complement(join(2772..2787,1..49)) /label=BbJ23150k-R ORIGIN 1 gacgtcttta cggctagctc agtcctaggt attatgctag caggtttaat cgaattcaaa 61 agatctttta agaaggagat atacatatgg cgagtagcga agacgttatc aaagagttca 121 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 181 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 241 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 301 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 361 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 421 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 481 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 541 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 601 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 661 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 721 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt 781 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 901 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc 961 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa 1021 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata 1081 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc 1141 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg 1201 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca 1261 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc 1321 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa 1381 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt 1441 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc 1501 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg 1561 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga 1621 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa 1681 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc 1741 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 1801 cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag 1861 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg 1921 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta 1981 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca 2041 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg 2101 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc 2161 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt 2221 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta 2281 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat 2341 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg 2401 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct 2461 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg 2521 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc 2581 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct 2641 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcatga tcccctgcgc 2701 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta 2761 ccagagggcg ccccagctgg caattcc //