LOCUS       Exported                2631 bp ds-DNA     circular SYN 26-MAR-2019
DEFINITION  Sourced from Addgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, 
            Keasling JD.
  TITLE     BglBricks: A flexible standard for biological part assembly.
  JOURNAL   J Biol Eng 2010;4:1.
  PUBMED    20205762
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Sandra
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 26, 2019 from SnapGene 4.3.4
            https://www.snapgene.com
COMMENT     Chloramphenicol resistance.
            Ptrc inducible promoter
            p15A origin of replication
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        7..41
                     /label=J23113
     primer_bind     35..71
                     /label=BbJ231xxF
     CDS             87..764
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      797..868
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      884..911
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1073..1618)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      1732..1826
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1847..2506)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2507..2609)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2556..2581
                     /label=pBbcpromseq
     primer_bind     complement(join(2610..2631,1..49))
                     /label=BbJ23113c-R
ORIGIN
        1 gacgtcctga tggctagctc agtcctaggg attatgctag caggtttaat cgaattcaaa
       61 agatctttta agaaggagat atacatatgg cgagtagcga agacgttatc aaagagttca
      121 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      181 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      241 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      301 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      361 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
      421 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      481 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      541 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      601 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      661 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
      721 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt
      781 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc
      961 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1021 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1081 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1201 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1261 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1321 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1381 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1441 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     1501 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     1561 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     1621 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     1681 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     1741 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1801 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1861 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1921 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1981 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2041 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2101 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2161 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2221 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2281 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2341 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2401 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2461 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2521 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2581 gaaagttgga acctcttacg tgccgatcac gtctcatttt cgccagatat c
//
