LOCUS       Exported                2645 bp ds-DNA     circular SYN 26-MAR-2019
DEFINITION  pBbE1c-RFP sequence 4051 bps
            Sourced from Addgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, 
            Keasling JD.
  TITLE     BglBricks: A flexible standard for biological part assembly.
  JOURNAL   J Biol Eng 2010;4:1.
  PUBMED    20205762
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 26, 2019 from SnapGene 4.3.4
            https://www.snapgene.com
COMMENT     Chloramphenicol reisistance
            Ptrc inducible promoter
            colE1 origin of replication
            RFP tagged
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..41
                     /label=J23150
     primer_bind     35..71
                     /label=BbJ231xxF
     CDS             87..764
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      797..868
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      884..911
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1069..1657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1745..1839
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1860..2519)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2520..2622)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(join(2624..2645,1..49))
                     /label=BbJ23150c-R
ORIGIN
        1 gacgtcttta cggctagctc agtcctaggt attatgctag caggtttaat cgaattcaaa
       61 agatctttta agaaggagat atacatatgg cgagtagcga agacgttatc aaagagttca
      121 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      181 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      241 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      301 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      361 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
      421 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      481 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      541 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      601 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      661 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
      721 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt
      781 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatacct agggcgttcg gctgcggcga gcggtatcag ctcactcaaa
      961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1501 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgactagtgc
     1741 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1801 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1861 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1921 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1981 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2041 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     2101 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2161 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2221 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2281 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2341 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2401 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2461 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2521 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2581 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     2641 atatc
//
