LOCUS       Exported                2311 bp ds-DNA     circular SYN 21-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Savage
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2019 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(172..1032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1033..1137)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1069..1100
                     /label=Fwd seq primer
     terminator      1227..1270
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1227..1270
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        1288..1322
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        1288..1317
                     /label=J23119
                     /label=J23119 (SpeI) promoter
     primer_bind     complement(1318..1375)
                     /label=HBS 68 extend triplex
     primer_bind     complement(1318..1375)
                     /label=HBS 66 change all triplex
     primer_bind     complement(1318..1342)
                     /label=HBS 61 fix bp on scaffold
     promoter        1322
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    1323..1430
                     /label=CasX gRNA
     misc_feature    1323..1403
                     /label=tracrRNA
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    1335..1337
                     /label=triplex
     primer_bind     complement(1341..1363)
                     /label=HBS 54 truncated scafold 1
     primer_bind     complement(1342..1365)
                     /label=HBS 56 truncated scaffold 2
     primer_bind     1343..1383
                     /label=HBS 60 fix bp scafold stem
     primer_bind     complement(1347..1367)
                     /label=HBS 58 truncate scaffold 3
     misc_feature    1348..1350
                     /label=triplex
     misc_feature    1357..1384
                     /label=scaffold
     misc_feature    1362..1363
                     /label=mismatch
     primer_bind     complement(1368..1389)
                     /label=HBS 77 change stretch stem cg
     primer_bind     complement(1372..1397)
                     /label=HBS 75 truncated stretch 3
     primer_bind     complement(1372..1395)
                     /label=HBS 73 truncated stretched 2
     primer_bind     complement(1372..1393)
                     /label=HBS 71 stretched stem truncation
     primer_bind     1373..1399
                     /label=HBS 59 truncated scaffold 3
     primer_bind     1375..1400
                     /label=HBS 57 truncated scaffold 2
     primer_bind     1376..1448
                     /label=HBS 69 extend triplex
     primer_bind     1376..1448
                     /label=HBS 67 triplex all change
     primer_bind     1377..1400
                     /label=HBS 55 truncated scaffold 1
     misc_feature    1386..1422
                     /label=stretched stem
     primer_bind     1398..1443
                     /label=HBS 76 change stretch stem cg
     primer_bind     complement(1400..1426)
                     /label=HBS 62 ggg change triplex
     misc_feature    1404..1407
                     /label=tetraloop
     misc_feature    1408..1430
                     /label=Repeat 1
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     primer_bind     1411..1436
                     /label=HBS 74 truncated stretch 3
     primer_bind     1413..1439
                     /label=HBS 72 truncated stretch 2
     primer_bind     1413..1439
                     /label=HBS 70 truncated stretched stem
     misc_feature    1427..1429
                     /label=triplex
     primer_bind     1430..1454
                     /label=HBS 65 ccc change triplex
     primer_bind     1430..1454
                     /label=HBS 64 uuu change triplex
     primer_bind     1430..1454
                     /label=HBS 63 ggg change triplex
     misc_feature    1431..1450
                     /label=spacer sequence
     misc_feature    complement(1694..1712)
                     /label=Rev seq primer
     rep_origin      complement(join(1724..2311,1))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
       61 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      181 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
      241 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
      301 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
      361 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      421 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      481 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      541 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      601 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      661 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      721 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      781 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      841 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      901 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      961 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1021 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1081 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1141 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     1201 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     1261 ggatgatttc tggaattcta aagatctttg acagctagct cagtcctagg tataatacta
     1321 gtacatctgg cgcgtttatt ccattacttt ggagccagtc ccagcgacta tgtcgtatgg
     1381 acgaagcgct tatttatcgg agagaaaccg ataagtaaaa cgcatcaaag ctggagttgt
     1441 cccaattctt ctagagtcac actggctcac cttcgggtgg gccctagagt cacactggct
     1501 caccttcggg tgtttctgcg ggatccttac tcgagtctag actgcaggct tcctcgctca
     1561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2281 agcagattac gcgcagaaaa aaaggatctc a
//
