LOCUS       pAAV_mL7-6_GFP_        8126 bp    DNA     circular SYN 02-NOV-2016
DEFINITION  Ligation of pAAV Vector GFP WPRE A17 AgeI/XhoI into mL7-6 Insert
            XhoI/AgeI
ACCESSION   pAAV_mL7-6_GFP_
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             6..849
                     /gene="L7-6"
     CDS             863..1582
                     /gene="EGFP"
     misc_feature    1594..2188
                     /gene="WPRE"
     CDS             2233..4123
                     /gene="HBGpA(1.9k)"
     misc_feature    4124..4309
                     /gene="ITR"
                     /product="ITR"
     misc_feature    7940..8125
                     /gene="ITR"
                     /product="ITR"
ORIGIN      
        1 tcgagggttc caccctcatg ttggttgatc ttcaacattg tcctgacttc ttcccacttg
       61 acattcctca gggtcctgtg atcatggctg ggtctagtga ggttcaaacc tgcactgccc
      121 tacccacacc cacacccagc tcagcgtcag tcaggatcaa cagttaccta gagatcatct
      181 ttctggggct taagcattgg tgggagcaga tgggatatga gctggggatt tggggatggg
      241 ggaagatatc tgctccccct ccccctacac cctagccttt taaaaggcct tctcaggtca
      301 gagaccagga gaaaagtata ggagagatac acaatggacc aggaagaaga aaagggagag
      361 ggaggctcag accttctaga caaggtaaga gggctctggc tgactccacc atccgcttct
      421 tgaggtctcg gcacctgtaa ttgacaagat taattcattt atagggcatc taattagcaa
      481 gtaagtctct ggagtcccct gacccagtta ctataacaca cagggggtat aggtagggga
      541 gtataagagc ccctcctcag ggcaaatgaa tggattctta gtactgtccc ccaagagata
      601 gtaggtacta ggatttaggg gcacttctga gccccatttc cctggtaagt gtcccaaccc
      661 cccaaatcaa cccaagcctg gtctcaatct aggacagtgg tagaatgctg tccctagagt
      721 cagtaccatg tgaaattgtg ctgcaggcag gggccccagg ctgggaggtg ggggttgggg
      781 gagtcagggg caggtcaggg aaggagactc aggtttcatt tagagaaatt ctgcagaccc
      841 gtgaggacta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      901 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      961 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1021 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1081 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1141 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1201 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1261 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1321 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1381 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1441 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1501 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1561 catggacgag ctgtacaagt aaagcggccg catcgataat caacctctgg attacaaaat
     1621 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1681 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1741 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     1801 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     1861 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     1921 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     1981 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     2041 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     2101 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     2161 gatctccctt tgggccgcct ccccgcatcg atgccgtata cggtacccgg ggatcctcta
     2221 gctagagtcg aggaattcac cccaccagtg caggctgcct atcagaaagt ggtggctggt
     2281 gtggctaatg ccctggccca caagtatcac taagctcgct ttcttgctgt ccaatttcta
     2341 ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct
     2401 tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa
     2461 ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa
     2521 gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga
     2581 aggtgaggct gcaaacagct aatgcacatt ggcaacagcc ctgatgccta tgccttattc
     2641 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg
     2701 tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc
     2761 ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt
     2821 cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca
     2881 gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat
     2941 ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc
     3001 tgcagtgcta gtctcccgga actatcactc tttcacagtc tgctttggaa ggactgggct
     3061 tagtatgaaa agttaggact gagaagaatt tgaaaggggg ctttttgtag cttgatattc
     3121 actactgtct tattacccta tcataggccc accccaaatg gaagtcccat tcttcctcag
     3181 gatgtttaag attagcattc aggaagagat cagaggtctg ctggctccct tatcatgtcc
     3241 cttatggtgc ttctggctct gcagttatta gcatagtgtt accatcaacc accttaactt
     3301 catttttctt attcaatacc taggtaggta gatgctagat tctggaaata aaatatgagt
     3361 ctcaagtggt ccttgtcctc tctcccagtc aaattctgaa tctagttggc aagattctga
     3421 aatcaaggca tataatcagt aataagtgat gatagaaggg tatatagaag aattttatta
     3481 tatgagaggg tgaaacctaa aatgaaatga aatcagaccc ttgtcttaca ccataaacaa
     3541 aaataaattt gaatgggtta aagaattaaa ctaagaccta aaaccataaa aatttttaaa
     3601 gaaatcaaaa gaagaaaatt ctaatattca tgttgcagcc gttttttgaa tttgatatga
     3661 gaagcaaagg caacaaaagg aaaaataaag aagtgaggct acatcaaact aaaaaatttc
     3721 cacacaaaaa agaaaacaat gaacaaatga aaggtgaacc atgaaatggc atatttgcaa
     3781 accaaatatt tcttaaatat tttggttaat atccaaaata tataagaaac acagatgatt
     3841 caataacaaa caaaaaatta aaaataggaa aataaaaaaa ttaaaaagaa gaaaatcctg
     3901 ccatttatgc gagaattgat gaacctggag gatgtaaaac taagaaaaat aagcctgaca
     3961 caaaaagaca aatactacac aaccttgctc atatgtgaaa cataaaaaag tcactctcat
     4021 ggaaacagac agtagaggta tggtttccag gggttggggg tgggagaatc aggaaactat
     4081 tactcaaagg gtataaaatt tcagttatgt gggatgaata aattctagag catggctacg
     4141 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     4201 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4261 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgg cgtaatagcg
     4321 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaatt
     4381 ccaacatcca ataaatcata caggcaaggc aaagaattag caaaattaag caataaagcc
     4441 tcagagcata aagctaaatc ggttgtacca aaaacattat gaccctgtaa tacttttgcg
     4501 ggagaagcct ttatttcaac gcaaggataa aaatttttag aaccctcata tattttaaat
     4561 gcaatgcctg agtaatgtgt aggtaaagat tcaaacgggt gagaaaggcc ggagacagtc
     4621 aaatcaccat caatatgata ttcaaccgtt ctagctgata aattcatgcc ggagagggta
     4681 gctatttttg agaggtctct acaaaggcta tcaggtcatt gcctgagagt ctggagcaaa
     4741 caagagaatc gatgaacggt aatcgtaaaa ctagcatgtc aatcatatgt accccggttg
     4801 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg
     4861 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4921 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4981 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5041 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5101 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5161 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5221 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5281 ttaatgcgcc gctacagggc gcgtactatg gttgctttga cgagcacgta taacgtgctt
     5341 tcctcgttag aatcagagcg ggagctaaac aggaggccga ttaaagggat tttagacagg
     5401 aacggtacgc cagaatcctg agaagtgttt ttataatcag tgaggccacc gagtaaaaga
     5461 gtctgtccat cacgcaaatt aaccgttgtc gcaatacttc tttgattagt aataacatca
     5521 cttgcctgag tagaagaact caaactatcg gccttgctgg taatatccag aacaatatta
     5581 ccgccagcca ttgcaacagg aaaaacgctc atggaaatac ctacattttg acgctcaatc
     5641 gtctggaatt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5701 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5761 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5821 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5881 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5941 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6001 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6061 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6121 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6181 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6241 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6301 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6361 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6421 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6481 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6541 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6601 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6661 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6721 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6781 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6841 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6901 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6961 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7021 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7081 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7141 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7201 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7261 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7321 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7381 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7441 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7501 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7561 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7621 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7681 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7741 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7801 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7861 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7921 gttggccgat tcattaatgc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa
     7981 gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga
     8041 gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta
     8101 cttatctacg tagccatgct ctagac
//
