LOCUS       pEGFP-N1-hMOG(alpha\1)        5465 bp    DNA     circular SYN
            
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green
            fluorescent protein (egfp) and neomycin phosphotransferase genes,
            complete cds.
ACCESSION   pEGFP-N1-hMOG(alpha\1)
VERSION     pEGFP-N1-hMOG(alpha\1).0
KEYWORDS    .
SOURCE      Cloning vector pEGFP-N1
  ORGANISM  Cloning vector pEGFP-N1
            .
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
                      1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     ORIGDB|GenBank
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
            
            This file is created by Vector NTI
            http://www.lifetechnologies.com/
            VNTAUTHORNAME|Demo User
            VNTOAUTHORNAME|UNKNOWN
FEATURES             Location/Qualifiers
     source          1..5465
                     /label=Source_1
                     /label=Source_1
                     /vntifkey="98"
                     /mol_type="genomic DNA"
                     /original_location="1..4733"
                     /organism="Cloning vector pEGFP-N1"
                     /db_xref="taxon:47945"
                     /db_xref="taxon:47945"
     gene            1411..2130
                     /label=egfp
                     /label=egfp
                     /vntifkey="60"
                     /gene="egfp"
                     /original_location="679..1398"
     CDS             1411..2130
                     /label=EGFP
                     /label=EGFP
                     /vntifkey="4"
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /original_location="679..1398"
                     /db_xref="GI:1377912"
     CDS             3361..4155
                     /label=Kan/Neo R
                     /label=Kan/Neo R
                     /vntifkey="4"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /original_location="2629..3423"
                     /db_xref="GI:1377913"
     polyA_signal    2284..2334
                     /label=SV40 PolyA
                     /label=SV40 PolyA
                     /vntifkey="25"
     rep_origin      2381..2836
                     /label=f1 origin
                     /label=f1 origin
                     /vntifkey="33"
     rep_origin      4740..5383
                     /label=pUC origin
                     /label=pUC origin
                     /vntifkey="33"
     eukaryotic      1..589
                     /label=CMV  promoter
                     /label=CMV  promoter
                     /vntifkey="29"
     CDS             619..1359
                     /label=hMOG
                     /label=hMOG
                     /vntifkey="4"
                     /original_location="896..1636"
     CDS             619..2127
                     /label=hMOG-EGFP
                     /label=hMOG-EGFP
                     /vntifkey="4"
     CDS             619..705
                     /label=leader peptide
                     /label=leader peptide
                     /vntifkey="4"
     CDS             1081..1146
                     /label=TM-domain 1
                     /label=TM-domain 1
                     /vntifkey="4"
     CDS             1252..1311
                     /label=TM-domain 2
                     /label=TM-domain 2
                     /vntifkey="4"
     CDS             1147..1251
                     /label=ic-loop
                     /label=ic-loop
                     /vntifkey="4"
     CDS             736..1050
                     /label=Ig-domain
                     /label=Ig-domain
                     /vntifkey="4"
     CDS             1312..1359
                     /label=loop 2
                     /label=loop 2
                     /vntifkey="4"
     protein_bind    895..909
                     /label=C1q binding
                     /label=C1q binding
                     /vntifkey="31"
     glycosil_site   796..798
                     /label=N30
                     /label=N30
                     /vntifkey="11"
     misc_feature    1234..1236
                     /label=Palmitoylation #1
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    1297..1299
                     /label=Palmitoylation #2
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagat ggcaagctta tcaagaccct ctctgcccag ctgcctctgc
      661 tccttcctcc tcctcctcct cctccaagtg tcttccagct atgcagggca gttcagagtg
      721 ataggaccaa gacaccctat ccgggctctg gtcggggatg aagtggaatt gccatgtcgc
      781 atatctcctg ggaagaacgc tacaggcatg gaggtggggt ggtaccgccc ccccttctct
      841 agggtggttc atctctacag aaatggcaag gaccaagatg gagaccaggc acctgaatat
      901 cggggccgga cagagctgct gaaagatgct attggtgagg gaaaggtgac tctcaggatc
      961 cggaatgtaa ggttctcaga tgaaggaggt ttcacctgct tcttccgaga tcattcttac
     1021 caagaggagg cagcaatgga attgaaagta gaagatcctt tctactgggt gagccctgga
     1081 gtgctggttc tcctcgcggt gctgcctgtg ctcctcctgc agatcactgt tggcctcgtc
     1141 ttcctctgcc tgcagtacag actgagagga aaacttcgag cagagataga gaatctccac
     1201 cggacttttg atccccactt tctgagggtg ccctgctgga agataaccct gtttgtaatt
     1261 gtgccggttc ttggaccctt ggttgccttg atcatctgct acaactggct acatcgaaga
     1321 ctagcagggc aattccttga agagctacga aatcccttcc gaattctgca gtcgacggta
     1381 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     1441 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1501 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1561 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1621 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1681 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1741 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1801 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1861 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1921 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1981 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2041 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2101 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     2161 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2221 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2341 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     2401 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2461 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2521 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2581 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2641 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2701 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2761 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2821 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2881 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2941 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3001 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3061 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3121 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3241 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3301 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3361 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3421 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3481 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3541 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3601 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3661 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3721 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3781 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3841 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     3901 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3961 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4021 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4081 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4141 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4201 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4261 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4321 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4381 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4441 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4501 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4561 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5461 tgcat
//
