LOCUS       pRSFDuet-v2_RsTA        7026 bp ds-DNA     circular     22-OCT-2014
DEFINITION  Arabidopsis 4CL1, Rhodobacter TAL.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      .
  ORGANISM              
COMMENT     (4CL promoter region fixed).
FEATURES             Location/Qualifiers
     exon            complement(3940..4751)
                     /label="Km resistance"
                     /note="Geneious name: Km resistance"
     intron          5807..6886
                     /label="lacI"
                     /note="Geneious name: lacI"
     gene            1864..3549
                     /note="Geneious name: 4CL (Arabidopsis thaliana, paralog
                     1)"
     gene            71..1747
                     /label="TAL (Rhodobacter sphaeroides)"
                     /note="Geneious name: TAL (Rhodobacter sphaeroides)"
     promoter        7010..2
                     /label="T7 promoter"
                     /note="Geneious name: T7 promoter"
     promoter        1778..1796
                     /label="T7 promoter"
                     /note="Geneious name: T7 promoter"
     misc_feature    complement(4980..5141)
                     /label="RSF1030 ori"
                     /note="Geneious name: RSF1030 ori"
     misc_feature    2..29
                     /label="LacO"
                     /note="Geneious name: LacO"
     misc_feature    1796..1823
                     /label="LacO"
                     /note="Geneious name: LacO"
     misc_feature    complement(3645..3663)
                     /label="T7 terminal primer"
                     /note="Geneious name: T7 terminal primer"
     misc_feature    83..100
                     /label="His 6x"
                     /note="Geneious name: His 6x"
     misc_feature    1850..1855
                     /label="RBS"
                     /note="Geneious name: RBS"
     misc_feature    58..63
                     /label="RBS"
                     /note="Geneious name: RBS"
     misc_feature    71..73
                     /label="start"
                     /note="Geneious name: start"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaaacctat
      121 gctcgccatg agcccaccaa aacctgccgt tgaactcgac cggcacattg atctggatca
      181 ggcgcatgcg gtcgcaagtg gaggcgcacg gatagtgctc gctccaccgg ctcgggaccg
      241 ctgtagggct tccgaagctc ggctcggtgc tgttatcagg gaggcccggc acgtgtatgg
      301 cctcacaacg gggttcggcc ctctcgccaa tcggctgatt tccggcgaaa atgttcgaac
      361 cctgcaggcc aacttggtgc atcacctggc ctcaggagtg ggacccgtgc tcgattggac
      421 taccgcgagg gccatggtcc tggctcgcct ggtctcaatc gctcaaggcg caagcggggc
      481 ctcagagggc accatcgcac gcttgattga cctcctcaat tctgagcttg ctcctgcagt
      541 gccttctcgg ggcaccgttg gcgccagtgg tgacctcacc cctctggcac acatggtgct
      601 ttgtttgcag ggcagaggag atttcctcga tagagatggc acacgcctgg acggagccga
      661 aggccttagg cgcggcaggc tccagcctct ggaccttagt catagggacg cgctggcatt
      721 ggtcaatggt acttcagcca tgacggggat tgccctcgta aacgcccacg catgtcgcca
      781 cctgggtaac tgggccgtgg ccttgacagc cctgcttgcc gagtgtctta gaggcagaac
      841 agaggcctgg gcagccgcac tcagcgacct gagaccccac ccgggtcaga aggatgctgc
      901 agctagactt agagctcggg tggatgggtc cgccagagtg gtcagacatg ttatcgcaga
      961 aaggcggctg gacgcagggg acatcggaac agagccagaa gccggacaag acgcctactc
     1021 tctccgctgc gccccacaag tgctgggagc cggatttgac accctggcct ggcacgaccg
     1081 ggtcctgact atagaactga acgccgtgac cgacaatcca gtgtttcctc cagatgggag
     1141 tgtgccggcg ctgcatgggg gaaactttat gggccagcac gtggccctta caagcgatgc
     1201 attggcaacc gccgttactg tcctggcagg actggcggag cgacagatcg cccgactgac
     1261 tgatgagagg ctgaatagag gtctgccccc cttcctgcac cgaggacctg ctgggttgaa
     1321 cagcgggttc atgggcgctc aggtaacagc cacggctctc cttgctgaga tgcgcgctac
     1381 agggcccgct tccattcata gcatctccac caacgcagcc aaccaggacg tcgtctctct
     1441 ggggaccatc gccgcaaggt tgtgccgaga aaagatcgac cgatgggctg agatactggc
     1501 tatcttggct ttgtgcctcg cacaggctgc ggaactgagg tgcggttccg gattggatgg
     1561 cgtgagcccc gctggtaaga agctggtaca ggccctgaga gagcagttcc caccccttga
     1621 gactgatagg ccccttggtc aggagattgc ggctctggct actcatctgc tgcaacagtc
     1681 tcccgtaggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta agtcgaacag
     1741 aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggggaa
     1801 ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga aggagatata
     1861 catatggccc cccaggaaca ggccgtgtct caggttatgg aaaagcaaag caataacaac
     1921 aactccgacg tcattttcag aagcaaactc cccgacatct atatacccaa ccacctctcc
     1981 ctgcacgact acatctttca gaacatctct gagtttgcca ctaaaccttg tctcatcaat
     2041 ggacccactg gacacgtgta tacatacagc gatgtgcacg tgatttctag gcagatcgcc
     2101 gcaaactttc acaagcttgg agtaaaccag aacgacgtgg tgatgctgct gcttcctaat
     2161 tgtccagaat ttgtgctgag tttcctggcc gctagcttca gaggagcaac agctacagcg
     2221 gcaaatccct tcttcacccc cgcagagata gcaaaacaag cgaaggcctc caacactaag
     2281 ctgatcatta ctgaggcaag gtatgtggat aagatcaaac cccttcagaa tgacgacgga
     2341 gtagtgattg tttgtataga cgataatgag tctgtgccaa tccccgaagg ctgcctgcgg
     2401 tttaccgagc tgacacaaag tacaactgaa gccagtgaag ttattgacag tgtggagatc
     2461 tctccagacg atgtggtggc acttccatac tcttccggca caaccggctt gccgaagggt
     2521 gtcatgctga cgcacaaagg attggtcacc agcgtcgcgc agcaagtcga tggcgaaaac
     2581 cccaatctct atttccacag cgacgacgtg atcctgtgcg tcctgcccat gttccatata
     2641 tacgcactca actcaatcat gctctgtggc ctgcgcgtgg gggccgcaat cttgattatg
     2701 cctaagtttg agatcaatct cctcctggag ctgattcagc gctgcaaggt aaccgtcgct
     2761 ccaatggtcc cgcccattgt gctggctatc gccaaaagca gcgagacaga gaagtacgat
     2821 ctttccagca tccgagtggt gaaatcaggg gcggctccac tgggaaagga gctggaagat
     2881 gccgtgaacg ccaagtttcc taacgctaaa cttggacaag gctacgggat gactgaagct
     2941 ggcccagtgc ttgccatgtc cttggggttc gccaaagagc catttcctgt taagtctggg
     3001 gcctgcggaa ccgtcgtccg gaatgcagaa atgaagatcg tcgacccgga tacaggtgat
     3061 tcactgtcac gaaatcagcc tggcgaaatt tgcatcaggg gtcatcagat catgaagggt
     3121 tacctcaata acccagctgc caccgccgaa acgattgata aggacggttg gctccatacc
     3181 ggggacattg gcttgatcga cgatgatgac gaactgttca tcgtggatcg cctgaaggag
     3241 ctcattaaat ataaagggtt ccaggttgcc cctgctgagc ttgaggcttt gttgataggc
     3301 catcctgata taaccgatgt tgccgttgtt gctatgaagg aggaggcggc tggggaggtt
     3361 cctgtagcct tcgtagtgaa aagtaaagac tcagagctca gtgaagacga cgtaaagcag
     3421 ttcgtgtcta agcaggtcgt cttttacaaa agaatcaaca aggtgttttt cacggagtcc
     3481 attccaaagg ctccttccgg taagattctg cgaaaagact tgcgggctaa gttggcaaat
     3541 ggcctgtgaa ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
     3601 ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact
     3661 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
     3721 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     3781 gacataagcg gctatttaac gaccctgccc tgaaccgacg acaagctgac gaccgggtct
     3841 ccgcaagtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3901 tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa
     3961 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     4021 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     4081 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     4141 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     4201 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     4261 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcgg
     4321 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     4381 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     4441 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     4501 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     4561 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     4621 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     4681 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     4741 atatggctca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggcat gcagcgctct
     4861 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggtgtca
     4921 gctcactcaa aagcggtaat acggttatcc acagaatcag gggataaagc cggaaagaac
     4981 atgtgagcaa aaagcaaagc accggaagaa gccaacgccg caggcgtttt tccataggct
     5041 ccgcccccct gacgagcatc acaaaaatcg acgctcaagc cagaggtggc gaaacccgac
     5101 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5161 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5221 tcatagctca cgctgttggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5281 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5341 gtccaacccg gtaagacacg acttatcgcc actggcagca gccattggta actgatttag
     5401 aggactttgt cttgaagtta tgcacctgtt aaggctaaac tgaaagaaca gattttggtg
     5461 agtgcggtcc tccaacccac ttaccttggt tcaaagagtt ggtagctcag cgaaccttga
     5521 gaaaaccacc gttggtagcg gtggtttttc tttatttatg agatgatgaa tcaatcggtc
     5581 tatcaagtca acgaacagct attccgttac tctagatttc agtgcaattt atctcttcaa
     5641 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgcc
     5701 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     5761 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     5821 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5881 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     5941 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     6001 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     6061 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
     6121 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     6181 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     6241 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     6301 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     6361 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     6421 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     6481 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     6541 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     6601 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     6661 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     6721 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     6781 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     6841 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     6901 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     6961 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
     7021 actata
//
