LOCUS       Exported                5707 bp ds-DNA     circular SYN 22-JUL-2019
DEFINITION  .
ACCESSION   New
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2109
                     /organism="Synthetic"
                     /label=source:Synthetic
                     /note="/locus_tag=source:Synthetic"
                     /note="color: #ffc0cb"
     source          2385..2424
                     /organism="Synthetic"
                     /label=source:Synthetic
                     /label=source:Synthetic(1)
                     /note="/locus_tag=source:Synthetic"
                     /note="color: #ffc0cb"
     source          2425..5707
                     /organism="BioBrick cloning vector pSB4K5-I52002"
                     /mol_type="other DNA"
                     /label=source:BioBrick cloning vector pSB4K5-I52002
                     /note="/locus_tag=source:BioBrick cloning vector 
                     pSB4K5-I52002"
                     /note="color: #0000ff"
                     /db_xref="taxon:508436"
     misc_feature    1..40
                     /locus_tag="Spacer0"
                     /product="Spacer0"
                     /label=Spacer0
                     /note="color: #ffc0cb"
     misc_feature    complement(41..760)
                     /locus_tag="sfGFP registery"
                     /product="sfGFP registery"
                     /label=sfGFP registery
                     /note="color: #21fe06; direction: LEFT"
     misc_feature    695..715
                     /locus_tag="Primer"
                     /label=Primer
                     /note="color: #00ffff"
     misc_feature    complement(761..766)
                     /locus_tag="Spacer RBS"
                     /product="Spacer RBS"
                     /label=Spacer RBS
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(767..778)
                     /locus_tag="B0034"
                     /product="B0034"
                     /label=B0034
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(779..854)
                     /locus_tag="BydvJ"
                     /product="BydvJ"
                     /label=BydvJ
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    855..907
                     /locus_tag="attB tp901"
                     /product="attB tp901"
                     /label=attB tp901
                     /note="color: #ffc0cb"
     misc_feature    complement(908..960)
                     /locus_tag="L3S3P21"
                     /product="L3S3P21"
                     /label=L3S3P21
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(961..1073)
                     /locus_tag="J61048"
                     /product="J61048"
                     /label=J61048
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    1074..1134
                     /locus_tag="attB-int5"
                     /product="attB-int5"
                     /label=attB-int5
                     /note="color: #00ff80"
     misc_feature    1135..1154
                     /locus_tag="sp20-1"
                     /product="sp20-1"
                     /label=sp20-1
                     /note="color: #ffc0cb"
     misc_feature    complement(1155..1204)
                     /locus_tag="attB bxb1"
                     /product="attB bxb1"
                     /label=attB bxb1
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    complement(1205..1240)
                     /locus_tag="P6 promoter"
                     /product="P6 promoter"
                     /label=P6 promoter
                     /note="color: #fb0106; direction: LEFT"
     misc_feature    complement(1241..1300)
                     /locus_tag="attP-int5"
                     /product="attP-int5"
                     /label=attP-int5
                     /note="color: #00ff80; direction: LEFT"
     misc_feature    1301..1375
                     /locus_tag="RiboJ"
                     /product="RiboJ"
                     /label=RiboJ
                     /note="color: #ffc0cb"
     misc_feature    1376..1387
                     /locus_tag="B0034(1)"
                     /product="B0034"
                     /label=B0034
                     /label=B0034(1)
                     /note="color: #ffc0cb"
     misc_feature    1388..1393
                     /locus_tag="Spacer RBS(1)"
                     /product="Spacer RBS"
                     /label=Spacer RBS
                     /label=Spacer RBS(1)
                     /note="color: #ffc0cb"
     misc_feature    1394..2086
                     /locus_tag="BFP-Lu"
                     /product="BFP-Lu"
                     /label=BFP-Lu
                     /note="color: #80ffff"
     misc_feature    2087..2109
                     /locus_tag="Spacer4 (BamhI)"
                     /product="Spacer4 (BamhI)"
                     /label=Spacer4 (BamhI)
                     /note="color: #ffc0cb"
     misc_feature    2087..2109
                     /label=sp4
                     /note="color: #ffff80"
     misc_feature    2110..2126
                     /label=spacer_4
                     /label=nonstandard type: spacer_4
                     /note="color: #ffc0cb"
     misc_feature    2127..2221
                     /label=B0014
                     /label=nonstandard type: B0014
                     /note="color: #ffc0cb"
     misc_feature    2222..2261
                     /label=spacer_5
                     /label=nonstandard type: spacer_5
                     /note="color: #ffc0cb"
     misc_feature    2262..2311
                     /label=attP_Tp901
                     /label=nonstandard type: attP_Tp901
                     /note="color: #ff0000"
     misc_feature    2312..2331
                     /label=sp20_4
                     /label=nonstandard type: sp20_4
                     /note="color: #ffc0cb"
     misc_feature    complement(2332..2384)
                     /label=attP_Bxb1
                     /label=nonstandard type: attP_Bxb1
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    2385..2424
                     /locus_tag="SpacerN"
                     /product="SpacerN"
                     /label=SpacerN
                     /note="color: #ffff00"
     misc_feature    2425..2426
                     /label=New Feature
                     /note="color: #ff0000"
     misc_feature    2425
                     /locus_tag="homology 3'"
                     /label=homology 3'
                     /note="color: #00ffff"
     misc_feature    5667..5707
                     /locus_tag="homology 5'"
                     /label=homology 5'
                     /note="color: #00ffff"
ORIGIN
        1 ctcggatacc cttactctgt tgaaaacgaa tagataggtt ttatcatttg tacagttcat
       61 ccataccatg cgtgatgccc gctgcggtta cgaactccag cagaaccata tgatcgcgtt
      121 tctcgttcgg atctttagac agaacgcttt gcgtgctcag atagtgattg tctggcagca
      181 gaacaggacc atcaccgatt ggagtgtttt gctggtagtg atcagccagc tgcacgctgc
      241 catcctccac gttgtggcga attttaaaat tcgctttaat gccatttttt tgtttatcgg
      301 cggtgatgta aacattgtgg ctgttaaaat tgtattccag cttatggccc aggatattgc
      361 cgtcttcttt aaagtcaatg cctttcagct caatgcggtt taccagggta tcgccttcaa
      421 atttcacttc cgcacgcgtt ttgtacgtgc cgtcatcctt aaaggaaatc gtgcgttcct
      481 gcacatagcc ttccggcatg gcggacttga agaagtcatg ctgcttcata tggtccggat
      541 aacgagcaaa gcactgaaca ccataagtca gcgtcgttac cagagtcggc caaggtaccg
      601 gcagtttacc agtagtacag atgaacttca gcgtcagttt accattagtt gcgtcacctt
      661 caccctcgcc acgcacggaa aacttatgac cgttgacatc accatccagt tccaccagaa
      721 tagggacgac accagtgaac agctcttcgc ctttacgcat ctagtatttc tcctcttttt
      781 aaacaaaatt atttgtagag gggtgtttcg tcctttcgga ctcatcagtc aaggtacgca
      841 ccttgagaca ccctatgcca acacaattaa catctcaatc aaggtaaatg ctttttgctt
      901 tttttgcggg agaccagaaa caaaaaaagg ccccccgtta gggaggcctt caataattgg
      961 tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa
     1021 acccgccgaa gcgggttttt acgtaaatca ggtgaaactg accgataagc cgggagcgcc
     1081 ggatcaggga gtggacggcc tgggagcgct acacgctgtg gctgcggtcg gtgctagttg
     1141 cgtctcaggg acccgcccgg atgatcctga cgacggagac cgccgtcgtc gacaagccgg
     1201 ccgatccaca aacattacga gccggatgat taattgtcaa cagatcaggg tgcgcaagtt
     1261 gtcaacgctc ccaggagagt tatcgacttg cgtattaggg agctgtcacc ggatgtgctt
     1321 tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaaaaaga
     1381 ggagaaatac tagatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     1441 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1501 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     1561 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     1621 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     1681 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1741 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     1801 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     1861 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     1921 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     1981 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     2041 ggcagtggcc agatactgcg acctccctag caaactgggg cactaaaggc aactgaaacg
     2101 attcggatcc tgtattacta ttcttatcac actggctcac cttcgggtgg gcctttctgc
     2161 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt
     2221 tactttatct gagaatagtc aatcttcgga aatcccaggt ggcgagtttt tatttcgttt
     2281 atttcaatta aggtaactaa aaaactcctt tactcaggtc tgccgtaagg ggggtttgta
     2341 ccgtacacca ctgagaccgc ggtggttgac cagacaaacc acgaattatt gaccacttcc
     2401 gagtagaatc gtgcttcagt aagaggctca ctcaaaggcg gtaatcagat aaaaaaaatc
     2461 cttagctttc gctaaggatg atttctgcta gtattattag aaaaactcat cgagcatcaa
     2521 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     2581 ctgtaatgaa ggagaaaact caccgaggca gttccaaaga atggcaaggt cctggtaacg
     2641 gtctgcgatt ccgacccgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     2701 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaagag
     2761 cttgtgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     2821 actcgcatca accaaaccgt tattcatgcg tgattgcgcc tgagcaagac gaaatacacg
     2881 atcgctgtta aaaggacaat tacaaacagg aatcgaatgt aaccggcgca ggaacacggc
     2941 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaaggctgt
     3001 tttcccagga atcgcggtgg tgagtaacca cgcatcatca ggagtacgga taaaatgctt
     3061 gatggtcggg agaggcataa actccgtcag ccagttgaga cggaccatct catctgtaac
     3121 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     3181 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     3241 atataaatca gcgtccatgt tggagtttaa gcgcggacgg gagcaagacg tttcccgttg
     3301 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     3361 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     3421 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag ctctagtagt tacattgtcg
     3481 atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct
     3541 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg
     3601 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     3661 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     3721 ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa
     3781 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     3841 tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     3901 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     3961 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     4021 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     4081 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     4141 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     4201 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     4261 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     4321 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     4381 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     4441 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     4501 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     4561 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     4621 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     4681 ataattacat gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     4741 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     4801 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     4861 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     4921 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     4981 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     5041 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     5101 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     5161 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     5221 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     5281 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     5341 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     5401 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     5461 tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagctcta gctccggcaa
     5521 aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     5581 cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag
     5641 gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag ttagccctta
     5701 gtgactc
//
