LOCUS Exported 11080 bp ds-DNA circular SYN 22-MAY-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11080) AUTHORS . TITLE Direct Submission JOURNAL Exported May 22, 2019 from SnapGene Viewer 4.1.6 http://www.snapgene.com COMMENT [DEFINITION]: synthetic circular DNA [LOCUS]: Exported [ACCESSION]: . [SOURCE]: 1..11080 [CDS]: 7379..8170 FEATURES Location/Qualifiers source 1..11080 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 529..552 /label=SV40 NLS1 /note="label=SV40 NLS1 - 529..552" misc_feature 568..633 /label=3XFLAG /note="label=3XFLAG - 568..633" misc_feature 718..4821 /label=dCas9_D10A_H840A /note="label=dCas9_D10A_H840A - 718..4821" misc_feature 4867..4872 /label=note=_NheI /note="label=NheI - note=""note=""""direct strand translation"""" - translation=""""AS"""""" - 4867..4872" misc_feature 4876..6030 /label=DNMT3L /note="label=DNMT3L - 4876..6030" misc_feature 6058..6105 /label=Nucleoplasmin NLS /note="label=Nucleoplasmin NLS - 6058..6105" misc_feature 6124..6180 /label=P2A /note="label=P2A - 6124..6180" misc_feature 6981..7196 /label=SV40 enhancer-pBabe /note="label=SV40 enhancer-pBabe - 6981..7196" misc_feature 7160..7237 /label=SV40 ORI /note="label=SV40 ORI - 7160..7237" CDS 7379..8170 /label=Kan/neoR /note="label=Kan/neoR - 7379..8170" primer_bind complement(8512..8532) /label=M13-rev /note="label=M13-rev - complement(8512..8532)" promoter complement(8565..8594) /label=lac /note="label=lac - complement(8565..8594)" rep_origin complement(8900..9528) /direction=LEFT /label=ColE1 origin /note="direction=LEFT - label=ColE1 origin - complement(8900..9528)" CDS complement(9680..10339) /label=AmpR /note="label=AmpR - complement(9680..10339)" promoter complement(10579..10607) /label=Amp prom /note="label=Amp prom - complement(10579..10607)" promoter 10984..430 /label=CMV promoter /note="label=CMV promoter - 10984..430" ORIGIN 1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg 421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag 541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat 601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt 661 accggaggga gtggtggaag cggcggttct ggtggctcag gcggcagcgg ccggccaatg 721 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt 781 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac 841 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc 901 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac 961 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg 1021 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat 1081 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag 1141 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg 1201 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc 1261 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc 1321 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg 1381 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt 1441 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat 1501 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag 1561 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg 1621 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg 1681 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag 1741 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga 1801 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa 1861 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa 1921 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct 1981 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag 2041 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga 2101 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc 2161 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat 2221 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat 2281 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct 2341 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg 2401 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc 2461 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt 2521 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc 2581 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat 2641 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg 2701 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat 2761 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct 2821 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac 2881 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt 2941 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc 3001 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg 3061 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt 3121 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac 3181 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatgctatc 3241 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat 3301 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat 3361 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact 3421 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt 3481 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc 3541 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag 3601 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac 3661 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat 3721 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg 3781 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat 3841 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca 3901 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg 3961 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag 4021 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca 4081 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac 4141 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag 4201 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt 4261 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac 4321 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag 4381 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac 4441 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa 4501 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc 4561 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc 4621 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct 4681 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag 4741 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac 4801 ctctctcagc tcggtggaga cggtggtagt ggaggttcag gaggatccgg ggggagcgga 4861 gggagcgcta gcatggcggc catcccagcc ctggacccag aggccgagcc cagcatggac 4921 gtgattttgg tgggatccag tgagctctca agctccgttt cacccgggac aggcagagat 4981 cttattgcat atgaagtcaa ggctaaccag cgaaatatag aagacatctg catctgctgc 5041 ggaagtctcc aggttcacac acagcaccct ctgtttgagg gagggatctg cgccccatgt 5101 aaggacaagt tcctggatgc cctcttcctg tacgacgatg acgggtacca atcctactgc 5161 tccatctgct gctccggaga gacgctgctc atctgcggaa accctgattg cacccgatgc 5221 tactgcttcg agtgtgtgga tagcctggtc ggccccggga cctcggggaa ggtgcacgcc 5281 atgagcaact gggtgtgcta cctgtgcctg ccgtcctccc gaagcgggct gctgcagcgt 5341 cggaggaagt ggcgcagcca gctcaaggcc ttctacgacc gagagtcgga gaatcccctt 5401 gagatgttcg aaaccgtgcc tgtgtggagg agacagccag tccgggtgct gtcccttttt 5461 gaagacatca agaaagagct gacgagtttg ggctttttgg aaagtggttc tgacccggga 5521 caactgaagc atgtggttga tgtcacagac acagtgagga aggatgtgga ggagtgggga 5581 cccttcgatc ttgtgtacgg cgccacacct cccctgggcc acacctgtga ccgtcctccc 5641 agctggtacc tgttccagtt ccaccggctc ctgcagtacg cacggcccaa gccaggcagc 5701 cccaggccct tcttctggat gttcgtggac aatctggtgc tgaacaagga agacctggac 5761 gtcgcatctc gcttcctgga gatggagcca gtcaccatcc cagatgtcca cggcggatcc 5821 ttgcagaatg ctgtccgcgt gtggagcaac atcccagcca taaggagcag gcactgggct 5881 ctggtttcgg aagaagaatt gtccctgctg gcccagaaca agcagagctc gaagctcgcg 5941 gccaagtggc ccaccaagct ggtgaagaac tgctttctcc ccctaagaga atatttcaag 6001 tatttttcaa cagaactcac ttcctcttta tccggtggag gctccggagg tggatctaaa 6061 aggccggcgg ccacgaaaaa ggccggtcag gcaaaaaaga aaaagggtgg tagtggaagc 6121 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 6181 gcggccgcat gaaagggttc gatccctacc ggttagtaat gagtttaaac gggggaggct 6241 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 6301 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 6361 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt 6421 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 6481 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg 6541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 6601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 6661 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 6721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 6781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 6841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 6901 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 6961 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 7021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 7081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 7141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 7201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 7261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 7321 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 7381 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 7441 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 7501 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 7561 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 7621 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 7681 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 7741 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 7801 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 7861 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 7921 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 7981 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 8041 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 8101 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 8161 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc 8221 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 8281 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 8341 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8461 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 8521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 8581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 8641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 8701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 8761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 8821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 8881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 8941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 9001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 9061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 9121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 9181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 9241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 9301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 9361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 9421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 9481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 9601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 9661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 9721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 9781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 9841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 9901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 10021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 10081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 10141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 10201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 10261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 10321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 10381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 10441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 10501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 10561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 10621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 10681 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata 10741 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 10801 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 10861 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt 10921 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 10981 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 11041 attgacgtca ataatgacgt atgttcccat agtaacgcca //