LOCUS       Exported               11080 bp ds-DNA     circular SYN 22-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2019 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA [LOCUS]: Exported [ACCESSION]: 
            . [SOURCE]: 1..11080 [CDS]: 7379..8170
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    529..552
                     /label=SV40 NLS1
                     /note="label=SV40 NLS1 - 529..552"
     misc_feature    568..633
                     /label=3XFLAG
                     /note="label=3XFLAG - 568..633"
     misc_feature    718..4821
                     /label=dCas9_D10A_H840A
                     /note="label=dCas9_D10A_H840A - 718..4821"
     misc_feature    4867..4872
                     /label=note=_NheI
                     /note="label=NheI - note=""note=""""direct strand 
                     translation"""" - translation=""""AS"""""" - 4867..4872"
     misc_feature    4876..6030
                     /label=DNMT3L
                     /note="label=DNMT3L - 4876..6030"
     misc_feature    6058..6105
                     /label=Nucleoplasmin NLS
                     /note="label=Nucleoplasmin NLS - 6058..6105"
     misc_feature    6124..6180
                     /label=P2A
                     /note="label=P2A - 6124..6180"
     misc_feature    6981..7196
                     /label=SV40 enhancer-pBabe
                     /note="label=SV40 enhancer-pBabe - 6981..7196"
     misc_feature    7160..7237
                     /label=SV40 ORI
                     /note="label=SV40 ORI - 7160..7237"
     CDS             7379..8170
                     /label=Kan/neoR
                     /note="label=Kan/neoR - 7379..8170"
     primer_bind     complement(8512..8532)
                     /label=M13-rev
                     /note="label=M13-rev - complement(8512..8532)"
     promoter        complement(8565..8594)
                     /label=lac
                     /note="label=lac - complement(8565..8594)"
     rep_origin      complement(8900..9528)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="direction=LEFT - label=ColE1 origin - 
                     complement(8900..9528)"
     CDS             complement(9680..10339)
                     /label=AmpR
                     /note="label=AmpR - complement(9680..10339)"
     promoter        complement(10579..10607)
                     /label=Amp prom
                     /note="label=Amp prom - complement(10579..10607)"
     promoter        10984..430
                     /label=CMV promoter
                     /note="label=CMV promoter - 10984..430"
ORIGIN
        1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
       61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag
      541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat
      601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt
      661 accggaggga gtggtggaag cggcggttct ggtggctcag gcggcagcgg ccggccaatg
      721 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt
      781 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
      841 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc
      901 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
      961 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
     1021 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
     1081 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
     1141 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
     1201 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
     1261 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
     1321 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
     1381 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
     1441 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
     1501 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
     1561 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
     1621 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg
     1681 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     1741 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     1801 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     1861 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     1921 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct
     1981 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     2041 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     2101 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc
     2161 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     2221 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     2281 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     2341 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     2401 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     2461 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     2521 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     2581 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     2641 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
     2701 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     2761 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
     2821 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     2881 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     2941 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     3001 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
     3061 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     3121 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     3181 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatgctatc
     3241 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     3301 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     3361 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     3421 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
     3481 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     3541 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     3601 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     3661 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     3721 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     3781 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     3841 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     3901 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     3961 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     4021 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     4081 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     4141 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     4201 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     4261 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     4321 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag
     4381 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     4441 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     4501 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     4561 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     4621 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     4681 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
     4741 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     4801 ctctctcagc tcggtggaga cggtggtagt ggaggttcag gaggatccgg ggggagcgga
     4861 gggagcgcta gcatggcggc catcccagcc ctggacccag aggccgagcc cagcatggac
     4921 gtgattttgg tgggatccag tgagctctca agctccgttt cacccgggac aggcagagat
     4981 cttattgcat atgaagtcaa ggctaaccag cgaaatatag aagacatctg catctgctgc
     5041 ggaagtctcc aggttcacac acagcaccct ctgtttgagg gagggatctg cgccccatgt
     5101 aaggacaagt tcctggatgc cctcttcctg tacgacgatg acgggtacca atcctactgc
     5161 tccatctgct gctccggaga gacgctgctc atctgcggaa accctgattg cacccgatgc
     5221 tactgcttcg agtgtgtgga tagcctggtc ggccccggga cctcggggaa ggtgcacgcc
     5281 atgagcaact gggtgtgcta cctgtgcctg ccgtcctccc gaagcgggct gctgcagcgt
     5341 cggaggaagt ggcgcagcca gctcaaggcc ttctacgacc gagagtcgga gaatcccctt
     5401 gagatgttcg aaaccgtgcc tgtgtggagg agacagccag tccgggtgct gtcccttttt
     5461 gaagacatca agaaagagct gacgagtttg ggctttttgg aaagtggttc tgacccggga
     5521 caactgaagc atgtggttga tgtcacagac acagtgagga aggatgtgga ggagtgggga
     5581 cccttcgatc ttgtgtacgg cgccacacct cccctgggcc acacctgtga ccgtcctccc
     5641 agctggtacc tgttccagtt ccaccggctc ctgcagtacg cacggcccaa gccaggcagc
     5701 cccaggccct tcttctggat gttcgtggac aatctggtgc tgaacaagga agacctggac
     5761 gtcgcatctc gcttcctgga gatggagcca gtcaccatcc cagatgtcca cggcggatcc
     5821 ttgcagaatg ctgtccgcgt gtggagcaac atcccagcca taaggagcag gcactgggct
     5881 ctggtttcgg aagaagaatt gtccctgctg gcccagaaca agcagagctc gaagctcgcg
     5941 gccaagtggc ccaccaagct ggtgaagaac tgctttctcc ccctaagaga atatttcaag
     6001 tatttttcaa cagaactcac ttcctcttta tccggtggag gctccggagg tggatctaaa
     6061 aggccggcgg ccacgaaaaa ggccggtcag gcaaaaaaga aaaagggtgg tagtggaagc
     6121 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
     6181 gcggccgcat gaaagggttc gatccctacc ggttagtaat gagtttaaac gggggaggct
     6241 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6301 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     6361 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     6421 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     6481 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     6541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6661 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     6721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6901 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6961 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7321 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     7381 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     7441 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7501 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     7561 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7621 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7681 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7741 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7801 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7861 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7921 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7981 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     8041 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     8101 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     8161 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     8221 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     8281 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     8341 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8461 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     9121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10681 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata
    10741 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    10801 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
    10861 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
    10921 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    10981 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11041 attgacgtca ataatgacgt atgttcccat agtaacgcca
//
