LOCUS       pOSV808                 6149 bp    DNA     circular SYN 17-APR-2019
DEFINITION  Cloning vector for the assembly of DNA and integration at the VWB attP site in Streptomyces chromosome; hygromycin resistance
ACCESSION   pOSV808
KEYWORDS    .
SOURCE      Synthetic construct
  ORGANISM  Cloning vector
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Aubry, C. Pernodet, J-L. and Lautru, S.
  TITLE	 A set of modular and integrative vectors for synthetic biology in Streptomyces  
  JOURNAL   Unpublished.
COMMENT     Derived from pOSV804; includes prefix (NsiI/NotI/NheI) and suffix (SpeI/NotI/AflII) regions for cloning 
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /note="prefix (NsiI/NotI/NheI sites for cloning)"
     regulatory      23..133
                     /regulatory class="promoter"
                     /note="p(ndh)* promoter"
     regulatory      142..153
                     /regulatory class="ribosome_binding_site"
                     /note="B0034"
     gene            160..825
                     /gene="amilCP"
                     /product="blue chromoprotein AmilCP"
     misc_feature    837..965
                     /note="B0015"
     regulatory      837..916
                     /regulatory class="terminator"
                     /note="rrnBT1"
     regulatory      925..965
			     /regulatory class="terminator"
                     /note="T7TE"
     misc_feature    966..981
                     /note="suffix (SpeI/Not/AflII sites for cloning)"
     misc_recomb     complement (1198..1242)
                     /note="attP"
     gene            complement (1400..2683)
                     /gene="Int VWB"
                     /product="integrase of VWB"
     misc_recomb     3339..3372
                     /note="FRT; Flp recombinase target site"
     oriT    	     3373..3817
                     /note="region of RP4 origin of transfer"
     gene            complement (4080..5078)
                     /gene="aph(7")"
                     /product="Hygromycin resistance"
     rep_origin      5206..6115
                     /note="p15A origin of replication"
     misc_recomb     6116..6149
                     /note="FRT; Flp recombinase target site"
ORIGIN      
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttatatacta gtagcggccg cttaaggtcg acccgtctga cgcgtgtggg gagcacgccg
     1021 gcccgctcgg tagacagcac tctacgcatt gtggctgctg tgcacatcac tctttcgagc
     1081 gacgtgatcc gcaggattcc gtggggatgt ggcatctatg tggcatccgg gtacgacaac
     1141 ggccccccgt tcgaatggaa cggggggccg ttgactccct gacctgcgat gcaggttgtg
     1201 cccccggcag gattcgaacc tgcgacaccc gctttaggag agaacgtaaa gcgatcatga
     1261 gcctcagtga gtctgagtga gtctcagtga gcctgtcgcc ataggggctt cacctgcata
     1321 aatgagtttc agtgagtctc ggtgagtctc agtgaggggg tgttgtggca tccctgtggc
     1381 atctgcgtgg catccggcgt cacaccgccc gcagcgttcg aacaccctcg gtctcgggag
     1441 ccatcgccgc ccgcaccttc gccgccacgt cggccggcga atgctggtac aaccacgtca
     1501 ccttcgaccc tcgctcgtga cccatcacgg tctgcgtgtc cttctcgtca atgcccacat
     1561 ccttgagcca cgtggcgaac acgtggcgaa ggtcgtgcac ccgcggccac cactccatgc
     1621 ggccggtgtc ccggttcttc acctcgcggg cgaggcctgc agccttgatt gcaggtatcc
     1681 atgtgcgccg gaagttgtgc cgggtcagga cgccgccctg tgggccgcgg aacacgagct
     1741 catccggcgc gaggtcgtac gggtcctcgc caatcggggt gatcgtggga gtcggccgcc
     1801 accgggtgac catggtgcgg atcgcgtcct cggcctgcgg cgtgagcggg accgtgcgga
     1861 acccggcgtt gctcttcggc gcaggcttcc ggaacagctt gccttcgtcg tcactgagca
     1921 cttccttcac cttgaggtgg tgggcgtcca ggtcgacgtt ctcccagcgc agcgccgtcg
     1981 cctcgcccca ccggagaccg gtgtgttcaa ggaagacgac gagcggccgg tagtacatcg
     2041 gcaggtgact gacgatcagc gcgcactgcg cgcggtcggg cggcatcagt tcgtccggat
     2101 gcttcgccgg cgcctcgcca atgtccaggt cggccgccgg gttgaacggg atacgccggc
     2161 cgtccttgac ggcggcccgg agcatcgagt tcagcacctc atgaaccttc ttccgggtgt
     2221 ggtagccctt cacctccttc gtgatccacg cctgcagctc gatgtactcc aagtcgcaga
     2281 gacgccactg cccccacttc ggctcgatgt gcgcggccca gttcgaccgc ttccggttgg
     2341 cggtcgtgac tgctcggtcc ggctgcgcct cccaccacag tttccaccac tgggacaggg
     2401 tgatctcgcc acgcttcggg tcggcgtacg tccgttcgcg gacctgcgtg cggatcttgt
     2461 cgaggtgcgc ctcggctgcc ttcttaccgc cgtccttgat cgcaaagcat ttctgcttct
     2521 ggttgccggc cgggtcgcgg taacgcgcct gccacgagcc ggtgcagtcg cgtcggcggt
     2581 tccgctctcc gtacttctcg ggcgggtact tctccatgca cagctggcag ccgcagctct
     2641 tcgccctcaa ctgccgtggg ttgttcgtcg ctctaggcgc catgggtcac ctgtccgctc
     2701 ctccgctgtt cgggaacgcg gggcaggatg tgtacggctg ccccacacca gcagactgca
     2761 ccgtccgggg gctgctctac agccagttct tcaaggacgg ctcgaagggc tacgagggaa
     2821 gcatcccggg ggagggtggc aggcagggtg accgtgtttc cagcggcgtc gtacggccgg
     2881 aagttgagcg ggtcgagagg cgcgaagcgg acacgaatgc acatgggtat cccccgggca
     2941 ggcaggcgac gtttgaccgt cggccatggg ggagggcagg ccgctcgacc gaccgtaccc
     3001 ccttgtaggt gaatatgcga ccactctgcg tgcaccaagt gcccgaggaa tcacggggag
     3061 tggtggagtc cattttcgtt agacccgcga ggcaggccga cggttgtacg ggcgtcggct
     3121 actgcttgtt ggactcgttc agcgcgctca tctcgatcag cttggaacgc tgctgctcct
     3181 cggtgagacc acggaacagg gtgagcaggc gttcctcggc gtcctcgtcg agcgggcccg
     3241 gcgcggcccg gcgggccgcg gcgaaaatgc gctcttcggt gaacttcggg tacgcctcat
     3301 ggagggcgcg aagctttcgc gagctcgaga cctgcaggga agttcctatt ctctagaaag
     3361 tataggaact tcgtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
     3421 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
     3481 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat
     3541 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
     3601 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     3661 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
     3721 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
     3781 tataatgacc ccgaagcagg gttatgcagc ggaaaagggt accacgtcgc ggtgagttca
     3841 ggctttttca tatctcattg cccccggacg agcgtctgct ccgccattcg ccgtccgccg
     3901 tgccaatcgg atcagccgtc caaatgcggg attttcgtta gtcggaggcc aaacggcatt
     3961 gagcgtcagc atatcatcag cgagctgaag aaagacaatc cccgatccgc tccacgtgtt
     4021 gccccagcaa tcagcgcgac cttgcccctc caacgtcatc tcgttctccg ctcatgagct
     4081 caggcgccgg gggcggtgtc cggcggcccc cagaggaact gcgccagttc ctccggatcg
     4141 gtgaagccgg agagatccag cggggtctcc tcgaacacct cgaagtcgtg caggaaggtg
     4201 aaggcgagca gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc gtcgagcagc
     4261 gcggccagga tctcgcggtc gccccggaag gcgttgagat gcagttgcac caggctgtag
     4321 cgggagtctc ccgcatagac gtcggtgaag tcgacgatcc cggtgacctc ggtcgcggcc
     4381 aggtccacga agatgttggt cccgtgcagg tcgccgtgga cgaaccgggg ttcgcggccg
     4441 gccagcagcg tgtccacgtc cggcagccag tcctccaggc ggtccagcag ccggggcgag
     4501 aggtagcccc acccgcggtg gtcctcgacg gtcgccgcgc ggcgttcccg cagcagttcc
     4561 gggaagacct cggaatgggg ggtgagcacg gtgttcccgg tcagcggcac cctgtgcagc
     4621 cggccgagca cccggccgag ttcgcgggcc agggcgagca gcgcgttccg gtcggtcgtg
     4681 ccgtccatcg cggaccgcca ggtggtgccg gtcatccggc tcatcaccag gtagggccac
     4741 ggccaggctc cggtgccggg ccgcagctcg ccgcggccga ggaggcgggg caccggcacc
     4801 ggggcgtccg ccaggaccgc gtacgcctcc gactccgacg cgaggctctc cggaccgcac
     4861 cagtgctcgc cgaacagctt gatcaccggg ccgggctcgc cgaccagtac ggggttggtg
     4921 ctctcgccgg gcacccgcag caccggcggc accggcagcc cgagctcctc cagggctcgg
     4981 cgggccagcg gctcccagaa ttcctggtcg ttccgcaggc tcgcgtagga atcatccgaa
     5041 tcaatacggt cgagaagtaa cagggattct tgtgtcacga cattgcactc caccgctgat
     5101 gacatcagtc gatcatagca cgatcaacgg cactgttgca aatagtcggt ggtgataaac
     5161 ttatcatccc cttttgctga tggagctgca catgaaccgg atcccctagc ggagtgtata
     5221 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
     5281 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
     5341 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     5401 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     5461 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
     5521 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
     5581 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     5641 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     5701 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
     5761 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
     5821 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
     5881 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     5941 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     6001 ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg
     6061 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtgaagt
     6121 tcctattctc tagaaagtat aggaacttc
//
