LOCUS       pOSV810                 5703 bp    DNA     circular SYN 17-APR-2019
DEFINITION  Cloning vector for the assembly of DNA and integration at the PhiC31 attP site in Streptomyces chromosome; kanamycin resistance
ACCESSION   pOSV810
KEYWORDS    .
SOURCE      Synthetic construct
  ORGANISM  Cloning vector
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Aubry, C. Pernodet, J-L. and Lautru, S.
  TITLE	 A set of modular and integrative vectors for synthetic biology in Streptomyces  
  JOURNAL   Unpublished.
COMMENT     Derived from pOSV802; includes prefix (NsiI/NotI/NheI) and suffix (SpeI/NotI/AflII) regions for cloning
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /note="prefix (NsiI/NotI/NheI sites for cloning)"
     regulatory      23..133
			     /regulatory class="promoter"
                     /note="p(ndh)* promoter"
     regulatory      142..153
                     /regulatory class="ribosome_binding_site"
                     /note="B0034"
     gene            160..825
                     /gene="amilCP"
                     /product="blue chromoprotein AmilCP"
     misc_feature    837..965
                     /note="B0015"
     regulatory      837..916
                     /regulatory class="terminator"
                     /note="rrnBT1"
     regulatory      925..965
                     /regulatory class="terminator"
                     /note="T7TE"
     misc_feature    966..981
                     /note="suffix (SpeI/Not/AflII sites for cloning)"
     gene            complement (1007..2848)
                     /gene="Int phiC31"
                     /product="integrase of phiC31 bacteriophage"
     misc_recomb     complement (2865..2903)
                     /note="attP"
     misc_recomb     3093..3126
                     /note="FRT; Flp recombinase target site"
     oriT    	     3127..3571
                     /note="region of RP4 origin of transfer"
     gene            complement (3834..4628)
                     /gene="aph"
                     /product="Kanamycin resistance"
     rep_origin      4760..5669
                     /note="p15A origin of replication"
     misc_recomb     5670..5703
                     /note="FRT; Flp recombinase target site"
ORIGIN      
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttatatacta gtagcggccg cttaagcagg cttcccgggt gtctcgctac gccgctacgt
     1021 cttccgtgcc gtcctgggcg tcgtcttcgt cgtcgtcggt cggcggcttc gcccacgtga
     1081 tcgaagcgcg cttctcgatg ggcgttccct gccccctgcc cgtagtcgac ttcgtgacaa
     1141 cgatcttgtc tacgaagagc ccgacgaaca cgcgcttgtc gtctactgac gcgcgccccc
     1201 accacgactt agggccggtc gggtcagcgt cggcgtcttc ggggaaccat tggtcaaggg
     1261 gaagcttcgg ggcttcggcg gcttcaagtt cggcaagccg ctcttccgcc ccttgctgcc
     1321 ggagcgtcag cgctgcctgt tgcttccgga agtgcttcct gccaacgggt ccgtcgtacg
     1381 cgcctgccgc gcggtcttcg tacagctctt caagggcgtt cagggcgtcg gcgcgctccg
     1441 caacaaggtt cgcccgttcg ccgctcttct caggcgcctc agtgagcttg ccgaagcgtc
     1501 gggcggcttc ccacagaagc gccaacgtct cttcgtcgcc ttcggcgtgc ctgatcttgt
     1561 tgaagatgcg ttccgcaacg aacttgtcga gtgccgccat gctgacgttg cacgtgcctt
     1621 cgtgctgccc aggtgcggac gggtcgacca ccttccggcg acggcagcgg taagagtcct
     1681 tgatcgattc ttccccgcgc ttcgaagtca tgacggcgcc acactcgcag tacagcttgt
     1741 ccatggcgga cagaatggct tgcccccggg aaagcccctt gccgcgcccc ctgccgtcca
     1801 accacgcctg aagctcatac cactcagcgg gctcgatgat cggtccgcaa tcaagctcga
     1861 ccggccggag cgtgatcggg tcgcgctgaa tgcggtaacc ctcaatcttc gtggtcggcg
     1921 tgccgtccgg cttcttcttg tagatcacct cagcggcgaa gcccgcaata cgcgggtccc
     1981 gaaggattcg cataacggtt gccgggtccc aggcgcttga agcggtcttc ttcccaatcg
     2041 tctcgccccg ggtcggcacg gcgtcagcgt ccatgcgctt acaaagcccc gtgatgctgc
     2101 ccgggtgaat ggcggcttga ctgcccggct tgaagggaag gtgtttgtgc gtcttgatct
     2161 cacgccacca ccaccggatt acgtcgggct cgaactcgaa gggtccggta aggggagtgg
     2221 tcgagtgcgc aagcttgttg atgacgacat tgaccattcg gccgttgcgc gtgatctcct
     2281 tcgtctccga aacaagctcg aagccgtaag gcgccttccc gccgacgtac ccgcccaatt
     2341 cgcgctgaag gttcttcgtg tcgagaatct tcgccgactt cagcgaagat tctttgtgcg
     2401 acgcgtcgag ccgcataatc aggtgaatca ggtccatgac gtttccctgc cggaagacgc
     2461 cttcctgagt ggaaacaatc gtcacgccca gggcgagcaa ttccgagaca atcggaatcg
     2521 cgtccatgac cttcaggcgc gagaagcgcg acacgtcata gacaatgatc atgttgagcc
     2581 gcccggcgcg gcattcgttc aggatgcgtt cgaactccgg gcgctccgcc gtcccgaacg
     2641 ccgacgtgcc cggcgcttcg ctgaaatgcc cgacgaacct gaaccggccc ccgtcgcgct
     2701 cgacttcgcg ctgaaggtcg gccgccttgt cttcgttggc gctacgctgt gtcgctgggc
     2761 ttgctgcgct cgaattttcg cgctcgcgcg actgacggtc gtaagcaccc gcgtacgtgt
     2821 ccaccccggt cacaacccct tgtgtcatgt cggcgaccct acgcccccaa ctgagagaac
     2881 tcaaaggtta ccccagttgg ggcactactc ccgaaaaccg cttctgacct gggaaaacgt
     2941 gaagccccgg ggcatccgct gagggttgcc gccggggctt cggtgtgtcc gtcagtacgg
     3001 gccatagagg ggcgtcgtgt aacccaccca gggcgattgc tccggcacgg ggaagccgcg
     3061 ccacgccttc gggacgtctg gaatcctgca gggaagttcc tattctctag aaagtatagg
     3121 aacttcgtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga
     3181 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
     3241 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
     3301 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
     3361 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
     3421 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
     3481 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
     3541 gaccccgaag cagggttatg cagcggaaaa gggtaccacg tcgcggtgag ttcaggcttt
     3601 ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa
     3661 tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg cattgagcgt
     3721 cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg tgttgcccca
     3781 gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg agctcagaag
     3841 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     3901 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     3961 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     4021 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4081 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4141 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4201 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4261 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     4321 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     4381 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     4441 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     4501 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     4561 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     4621 tcaatcatgc gagacattgc actccaccgc tgatgacatc agtcgatcat agcacgatca
     4681 acggcactgt tgcaaatagt cggtggtgat aaacttatca tccccttttg ctgatggagc
     4741 tgcacatgaa ccggatcccc tagcggagtg tatactggct tactatgttg gcactgatga
     4801 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     4861 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     4921 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     4981 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     5041 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     5101 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     5161 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     5221 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     5281 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     5341 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     5401 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     5461 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     5521 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     5581 tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc
     5641 tgaagtcagc cccatacgat ataagttgtg aagttcctat tctctagaaa gtataggaac
     5701 ttc
//
