LOCUS       Exported               11315 bp ds-DNA     circular SYN 19-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   LGB
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 19, 2019 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1459..6324
                     /organism="Bacteroides ovatus ATCC 8483"
                     /strain="ATCC 8483"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     source          6325..6341
                     /organism="Bacteroides ovatus ATCC 8483"
                     /strain="ATCC 8483"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     source          6342..8189
                     /organism="Bacteroides ovatus ATCC 8483"
                     /strain="ATCC 8483"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     source          8291..9346
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(30..890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        1150..1458
                     /label=Pbt1311
     gene            1459..4542
                     /locus_tag="BACOVA_04505"
                     /label=BACOVA_04505
     CDS             1459..4542
                     /codon_start=1
                     /locus_tag="BACOVA_04505"
                     /product="TonB-linked outer membrane protein, SusC/RagA 
                     family"
                     /label=BACOVA_04505
                     /note="COG: NOG24971 non supervised orthologous group; 
                     Psort location: OuterMembrane, score:9.49"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR002048"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /inference="protein motif:same species:IPR000531"
                     /inference="protein motif:same species:IPR012910"
                     /inference="protein motif:same species:IPR002048"
                     /inference="protein motif:same species:IPR008969"
                     /inference="similar to AA sequence:same 
                     species:YP_101798.1"
                     /protein_id="EDO10124.1"
                     /translation="MLSIMKNKKLLCSVCFLFTFMSVLWGQNITVKGNVTSKTDGQPII
                     GASVVQNDSKSTGTITDLDGNFTLSVPTNALLAISYIGYKEVIIAAKPSLKIVMEEDAK
                     MIDEVVVTGYMAEKKASLTGSVAVVKMKEVADIPTGNVMSGLQGRVAGMNVTTDGKPGG
                     GNTDTKLRGITTINNSSPLYVIDGVQTHDNVASIISSNDVESIQVLKDAASAAIYGAQA
                     ANGVIIITTKRAKEGDVKVSFDMSLTAQTFTGGIDMLDAYQWGDVYWQAYKNTFGTNPN
                     SVVYGNGEKAQLQEYYFDQNGVKIKSGNTDWAKEIFGTALMQNYNLSLSKGFKDGNVAL
                     TLNYMNQDGLCRNTDYERFNSRLASNFRFVDGKVRVGESISVNHWLEHLNPAGIEELVI
                     AQHPAIPVYDENGGYAGGYVDILGDKPNAIRLTDNEANNRHKSWRIFGNVYLEVEPIKN
                     LVLKSQFGLNYTTGFNSTFVPKWSEADRKVDVNELTVRQDNSVQWVWSNTANYSIDFGK
                     NNISAVIGTEAKKENGEHLQGYGRGLVIEDLDYRYLDTVTEGKSVSNNASTYAMVSYFG
                     KVNYAFDDKYLISGTVRRDASSRFGKGNNSAIFPSVSAGWRVSREKFMECTQNWLSDLK
                     LRASWGINGNDQIDNNATYNLYYTNMSNGSYNFNGDGTTVLPGVQKDRSGNNDLKWEET
                     KQLNFGIDAAFFDNRLGLTLDYFQKKTTGMLIQKPYIGVIGEGGYKWYNAASMDNSGVE
                     ATFTWRDEIKDFKYDISFNLSYYKNEITELPDVIKYTWGGGNGVDKTIVGQPYGSWMSY
                     KADGVFKTKQEVYEYLSKYDVQIGAPGVGRIRYKDLNGDNIINTADMDWQGSDQPRFIG
                     GLNIGASYKGFDLSVFFNGMIRDAWNNSKFYTDLFQGWTGNHSVRLMDAMHAWNAYEQT
                     GVYNCDIPALTVVDNNVETRSSTFFIEDGSYVKLKTLTVGYTFPDKWIKKAHLSNLRLY
                     LQAQNVFTLTNYTGADPEGLGYPYPQPRTYTFGLSLGF"
     gene            4567..6324
                     /locus_tag="BACOVA_04504"
                     /label=BACOVA_04504
     CDS             4567..6324
                     /codon_start=1
                     /locus_tag="BACOVA_04504"
                     /product="SusD family protein"
                     /label=BACOVA_04504
                     /note="COG: NOG27574 non supervised orthologous group"
                     /db_xref="InterPro:IPR012944"
                     /inference="protein motif:same species:IPR012944"
                     /inference="similar to AA sequence:same 
                     species:YP_860092.1"
                     /protein_id="EDO10123.1"
                     /translation="MKLKYIFAVGVMALLASCDESRFLDIKPQGTLNDDLLSSADGVEL
                     LVTSAYAGLKGPNRDMHWVPMTNWTYGEVRSDNAYKGGGGVNDGDFCTLLETFKIDATW
                     GNADEKWFQLYCCLQRCNSALRVMNTLDENALPMIKSRKAEMKVIRAHFFFELVRLFKQ
                     VPYFDENVENDDYKYIPNNQFTREELLGKIAQEFLDAANDLPDTQSQVGRVTKNIAYAY
                     AAKARLYQAYRQDDNNQVVAIDKQLLAEVVTLCDKVGAQGKAYTLLNDFQKLDQLEYEN
                     GVESVFAVQYSMDDETDGAGNINWSNLLNAPKGPYGGDGFFLPSQNLINAYKTDNYGLP
                     LFDTFNADDYGIYEGSELTNIDATVDPRLDFVTGRPGITWKTYTVEPCKANWIRNSGEY
                     GFNCTKRFYISPESEDMFQGWPWGASHLNWQIIRYADVLLWKAEALIEIGEATGLEEAR
                     KIINRIRLRAKNSPYVKDFEHSEQDAANYLIGEYPAEGWSQDFARKALRWERRMEFAME
                     GDRFFDLVRWGIAAETMNSYISVEQNKRVYYRGARFVRGRDEYLPIPNAQYNFSEGNYV
                     QNPGYGKFN"
     gene            6342..8189
                     /locus_tag="BACOVA_04501"
                     /label=BACOVA_04501
     CDS             6342..8189
                     /codon_start=1
                     /locus_tag="BACOVA_04501"
                     /product="glycosyl hydrolase family 32"
                     /label=BACOVA_04501
                     /note="KEGG: bth:BT1759 5.6e-280 levanase precursor 
                     (2,6-beta-D-fructofuranosidase) K01238; COG: COG1621 
                     Beta-fructosidases (levanase/invertase)"
                     /db_xref="InterPro:IPR001362"
                     /db_xref="InterPro:IPR011040"
                     /db_xref="InterPro:IPR013148"
                     /db_xref="InterPro:IPR013189"
                     /inference="protein motif:same species:IPR013148"
                     /inference="protein motif:same species:IPR013189"
                     /inference="protein motif:same species:IPR001362"
                     /inference="protein motif:same species:IPR001362"
                     /inference="protein motif:same species:IPR011040"
                     /inference="similar to AA sequence:same 
                     species:NP_810672.1"
                     /protein_id="EDO10120.1"
                     /translation="MNVLFSFKQVQTFLTILVMMIFSFLDASADTSSLLIKDLGEGHCL
                     VQINTNQRYLLLPVEEVMPDVRVSMIVNNKEVNVADVRLAVNRVDYFVPLDLSAYAGKN
                     ILLKFKLGSNDPIRGKLSAVCCKEMKLSDTFDTGNREKFRPTYHFSPLYGWMNDPNGMV
                     YKDGEYHLFYQYNPYGSKWGNMSWGHAISQDLVNWKHLPVAIAPDALGTIFSGSAVVDF
                     DNTAGFGAGAIIAIYTQNSDRQVQSIAYSTDNGRTFTKYENNPVLTSEARDFRDPKVFW
                     YENTKRWIMVLAVGQEMQIFSSPNLKDWTFESRFGEGQGAHGGVWECPDLFELPVEGTN
                     DKKWVLLCNLNPGGPFGGSATQYFVGSFNGKEFVNEFPSKTKWMDWGKDHYATVTWSDA
                     PDNRRIAIAWMSNWQYANDVPTSQYRSPNSVPRDLSLFTVDGETYLQSAPSPELLALRD
                     ASKKRSFKVNGTRTIKEMIPGNDGAYEIELTIENQRADVIGFRLYNDKGEEVDMQYDMK
                     EKKFSMDRRKSGEVSFNENFPMLTWTAIEQGGNEEQGGKEALKLRLFVDKSSVEAFGNG
                     GRFVMTNQVFPSEPYNHINFYSKGGAYKVDSFVVHKLKP"
     gene            complement(8291..9346)
                     /label=ss-1988
                     /label=ss-1988-his
     misc_feature    9357..9375
                     /label=GH023 ribosome binding site
                     /note="ApEinfo_fwdcolor=#97eaff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#97eaff"
     misc_feature    9377..9395
                     /label=tetR binding site
                     /note="ApEinfo_fwdcolor=#ffc061
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ffc061"
     misc_feature    9396
                     /label=+1 txn initiation site
                     /note="ApEinfo_fwdcolor=#ffd544
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ffd544"
     misc_feature    9401..9408
                     /label=-7 binding site
                     /note="ApEinfo_fwdcolor=#ff7e51
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff7e51"
     misc_feature    9430..9433
                     /label=-33 binding site(1)
                     /note="ApEinfo_fwdcolor=#ff764f
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=-33 binding site
                     ApEinfo_revcolor=#ff764f"
     misc_feature    9439..9457
                     /label=tetR binding site(1)
                     /note="ApEinfo_fwdcolor=#ffc061
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=tetR binding site
                     ApEinfo_revcolor=#ffc061"
     misc_feature    9542
                     /label=mutation relative to parent vector
                     /note="has 7 T rather than 6 in the parental vector, but 
                     doesn't seem to affect regulation"
     misc_feature    9647..9650
                     /label=-33 binding site
                     /note="ApEinfo_fwdcolor=#ff764f
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff764f"
     misc_feature    9671..9678
                     /label=-7 binding site(1)
                     /note="ApEinfo_fwdcolor=#ff511e
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=-7 binding site
                     ApEinfo_revcolor=#ff511e"
     misc_feature    9683..9684
                     /label=+1 txn intiation site
                     /note="ApEinfo_fwdcolor=#ffbd5c
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ffbd5c"
     misc_feature    9685..9727
                     /label=A21 RBS
                     /note="ApEinfo_fwdcolor=#fd32ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#fd32ff"
     misc_feature    9728..10351
                     /label=tetR
                     /note="ApEinfo_fwdcolor=#fc1f14
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#fc1f14"
     rep_origin      10546..10934
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            11105..11214
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagagt gatctggaag aagcaatgaa agctgctgtt aagtctccga atcaggtatt
     1201 gttcctgaca ggtgtattcc catccggtaa acgcggatac tttgcagttg atctgactca
     1261 ggaataaatt ataaattaag gtaagaagat tgtaggataa gctaatgaaa tagaaaaagg
     1321 atgccgtcac acaacttgtc ggcattcttt tttgttttat tagttgaaaa tatagtgaaa
     1381 aagttgccta aatatgtatg ttaacaaatt atttgtcgta actttgcact ccaaatctgt
     1441 ttttaacata tggcactaat gcttagtatt atgaagaaca aaaaacttct ttgtagcgtt
     1501 tgtttcctgt ttactttcat gtcagtcctt tgggggcaaa acataacagt gaaaggaaac
     1561 gttacttcaa agactgatgg tcaaccaatt attggtgctt ccgtagtgca aaatgattct
     1621 aaatcaactg gaactatcac agatttggat ggtaacttta ctcttagtgt accaacgaat
     1681 gcattattgg caatttctta tataggatat aaagaagtaa ttattgccgc taaaccttca
     1741 ttgaagatag tgatggaaga ggatgctaag atgattgacg aagtggttgt gacaggttat
     1801 atggctgaaa agaaagccag tctgacaggt tcggtagctg tagtgaagat gaaagaggtg
     1861 gctgacatac ctactggtaa tgtgatgtca ggtttgcagg gacgtgtagc cggtatgaac
     1921 gtaacaactg atggtaaacc cggaggtggt aacacagata ctaagttacg tggtattact
     1981 accatcaaca attcttctcc tctttatgta atagacggtg ttcagacaca tgataatgtg
     2041 gcttctatta tctcatccaa tgatgttgaa tccattcagg tgttgaaaga tgcagcttcg
     2101 gctgccattt atggtgcgca agctgccaat ggtgtcatta ttatcaccac aaaacgtgct
     2161 aaggaaggcg atgtaaaagt aagttttgat atgtcgctca ctgcccagac ttttactggt
     2221 ggtattgata tgcttgacgc ttaccaatgg ggtgatgtgt attggcaggc ttataaaaat
     2281 acttttggaa ccaatcccaa tagtgtagta tatggaaatg gagaaaaggc ccaactgcaa
     2341 gaatattatt ttgaccagaa tggagtgaaa ataaagtcgg gcaacactga ttgggcaaag
     2401 gaaatatttg gtacggcttt aatgcaaaat tataatcttt ctctttctaa agggtttaag
     2461 gatgggaatg tggctcttac cttaaattat atgaatcagg atggtctttg ccgtaataca
     2521 gactacgaac gttttaattc gcgtttagcc tctaattttc gctttgtaga tggtaaagtg
     2581 cgtgtgggtg aaagtatttc ggtgaatcac tggttggagc atcttaatcc tgccggtata
     2641 gaagaattgg ttattgccca gcatccggcc attcctgtgt atgatgaaaa tggagggtat
     2701 gccggtggct atgtggatat attgggggat aagccgaatg ccattcgctt gacagacaat
     2761 gaagccaata atcgtcacaa aagttggcgt atctttggca atgtttattt ggaagtggaa
     2821 cccattaaaa atctagtgtt gaagagtcaa tttggtttga actatactac cggtttcaac
     2881 tcaactttcg tccccaaatg gagtgaagcc gatcgtaagg tagatgtaaa tgaactgacc
     2941 gtacgacaag ataatagcgt gcaatgggta tggtcgaaca ctgccaatta tagtatagac
     3001 tttgggaaga acaatatcag tgcagtgata ggaactgaag ctaagaaaga gaatggtgaa
     3061 catttacagg gatatggtcg cggattggtg atagaagatc tcgattatcg ttacttagac
     3121 actgtaactg aaggaaaaag tgtgagtaac aatgcttcta cctatgctat ggtctcttac
     3181 tttggtaagg tgaactatgc gtttgatgat aaatacctta tatcaggaac agtgcgtcgt
     3241 gatgcttctt cacgtttcgg taaagggaat aattctgcta tctttccttc agtatctgcc
     3301 ggttggagag tatcgcgtga aaaatttatg gaatgtacgc aaaactggct ttcagatctt
     3361 aaattgcgtg catcgtgggg gatcaatggt aatgatcaga ttgataacaa tgctacttac
     3421 aacctctatt acactaacat gtccaatggc tcatacaatt tcaatgggga tggtacaacg
     3481 gtattgcccg gagtacagaa agaccgttcg ggtaacaacg acctgaaatg ggaagagacc
     3541 aaacaactga actttggtat tgatgctgct ttctttgaca accgtttggg actgacactg
     3601 gattattttc aaaaaaagac tactggtatg ttaatccaga aaccttatat aggagtgatc
     3661 ggtgaaggtg gatataaatg gtacaatgct gccagtatgg ataatagcgg tgtggaagca
     3721 acctttactt ggcgagatga aataaaggat ttcaagtatg atatttcgtt caacctttcc
     3781 tattataaaa atgaaattac cgaacttccg gatgtaatca aatatacttg gggaggtggt
     3841 aatggtgtgg acaaaactat tgtaggtcaa ccttacggat catggatgtc ctataaagct
     3901 gatggtgtat tcaaaacaaa acaggaggta tatgaatacc taagcaaata tgatgtacag
     3961 attggtgctc ccggcgtagg acgtattcgc tacaaagacc ttaacggtga taacattatc
     4021 aatactgctg atatggattg gcaagggagt gaccaaccta gatttattgg tggattgaac
     4081 atcggtgcat cttacaaagg atttgacctt tctgtattct tcaatggtat gattcgcgat
     4141 gcatggaaca attccaagtt ttataccgac ctttttcaag gttggacagg taatcactct
     4201 gtacgtttga tggatgcaat gcatgcttgg aatgcttatg agcaaacggg agtttataat
     4261 tgtgatatcc ctgcattgac tgtagttgat aataatgtag aaacacgcag ttccactttc
     4321 tttattgaag atggttctta tgtgaagttg aaaactctaa ctgtgggtta tactttcccg
     4381 gataagtgga ttaagaaagc gcatctttcc aatttgcgtt tgtatctgca agcacagaac
     4441 gtttttacgc tgactaatta tacgggtgcc gacccggaag gattgggcta tccatatccc
     4501 caacctcgta cctatacatt cggtctatca cttggttttt aatttttatc ctaaatacag
     4561 aacattatga aattgaaata tatctttgca gtaggtgtga tggcactatt ggcaagttgc
     4621 gatgagagtc gttttctaga tattaaacct cagggtacac tcaatgacga tttgttatca
     4681 tcggccgatg gtgtagagct tttggttact tcagcttatg caggattgaa aggtcctaat
     4741 cgtgatatgc actgggtgcc gatgaccaat tggacgtatg gtgaagtacg ttcggataat
     4801 gcctacaaag gtggtggcgg tgtaaatgat ggagactttt gtacgctgct tgaaaccttt
     4861 aaaatagatg ctacatgggg caatgccgac gaaaaatggt ttcaacttta ttgttgcttg
     4921 caacgttgta acagcgcatt gcgggtaatg aatacgcttg atgagaacgc tttgccgatg
     4981 attaaaagtc gtaaagccga aatgaaagtg attcgtgcac acttcttctt tgaactggtt
     5041 cgccttttca agcaagtacc ttattttgat gagaatgtgg aaaatgatga ctataagtac
     5101 attcccaaca atcagtttac ccgtgaagaa cttctgggta aaatagcaca agaatttctt
     5161 gatgctgcta acgatttgcc tgatactcaa tctcaagtag ggcgtgtcac taagaacatt
     5221 gcttatgctt atgctgccaa agctagactt tatcaggctt accgacaaga tgataataac
     5281 caagtggttg ctattgataa acaattgtta gctgaagtgg tgactctttg cgacaaagta
     5341 ggtgcgcaag gaaaggcata tactcttttg aacgattttc agaaacttga tcaattggaa
     5401 tatgaaaatg gggtagagtc tgtgtttgct gtacaatatt cgatggatga tgaaaccgat
     5461 ggtgccggga atataaactg gagtaatctg ttgaatgctc ctaaaggtcc ttatggtggc
     5521 gacggatttt tcttacccag tcagaacttg atcaatgcct ataagacaga taactatgga
     5581 ttgcctttgt ttgatacttt caacgccgat gactatggaa tatacgaagg aagtgagctc
     5641 accaacattg atgcaactgt ggatcctcgt ttggatttcg ttaccggtcg tcctggtatc
     5701 acttggaaaa catataccgt tgagccttgt aaagcaaact ggatacgtaa cagcggtgaa
     5761 tatggcttta attgtacaaa gcgcttctac atctcgcctg aaagtgaaga tatgttccaa
     5821 ggttggcctt ggggagcttc tcatctcaac tggcagataa ttcgctatgc tgatgtgttg
     5881 ttatggaagg ctgaagcctt gatagagatt ggcgaagcta ccggactgga agaggctcgc
     5941 aaaattatta accgtattcg tctgcgtgcc aaaaatagtc cttatgtaaa agactttgaa
     6001 cattccgaac aggatgcagc taattatctg ataggcgaat atcctgctga gggctggagt
     6061 caggactttg cccgcaaggc attacgttgg gaacgccgta tggagtttgc tatggaaggt
     6121 gaccgtttct tcgacttggt acgttggggg attgctgcgg aaaccatgaa cagctacatt
     6181 agtgtggagc agaataagcg cgtttattat cgtggtgctc gctttgttcg tggcagagat
     6241 gaatatttgc ctattcccaa tgcacaatat aacttctctg aaggcaatta tgtgcagaat
     6301 cccggatacg gtaagtttaa ctagctctaa taccatctga catgaatgta ttattctcgt
     6361 ttaaacaagt acagaccttt ttgactatcc tggttatgat gattttctca tttttggatg
     6421 cttcggccga tacttcttcc ttattaataa aagatttggg tgaaggacat tgcctggtac
     6481 agataaatac aaatcaacgt tatttgcttt tacctgtaga ggaggtaatg cctgatgtac
     6541 gtgtcagcat gatcgttaat aataaagagg tgaatgttgc ggatgttcgt ctggcagtga
     6601 acagagtgga ctattttgtt ccgctggatc tttcggctta tgctggtaaa aacatactgt
     6661 tgaaatttaa attaggctcg aatgatccta tacgggggaa actttctgct gtttgttgca
     6721 aggaaatgaa attgtccgac acgttcgata cgggcaaccg tgaaaagttc cgtcctactt
     6781 atcatttctc acctctttat gggtggatga atgatcctaa tggaatggta tataaagacg
     6841 gtgaatacca ccttttctat caatacaatc cttatggttc caaatgggga aatatgagtt
     6901 ggggacatgc tatcagccaa gatcttgtaa actggaaaca tcttcctgta gctattgctc
     6961 ccgatgcatt ggggacaatt ttcagtggct cggcagttgt cgattttgat aatacagccg
     7021 gttttggtgc aggtgccatt atcgccatct atacacaaaa cagtgaccga caagtacaaa
     7081 gcattgctta cagtactgac aacggacgta ctttcaccaa atatgagaat aatccggtac
     7141 tgacgtccga agcgcgtgac ttccgcgacc cgaaagtatt ttggtatgaa aacaccaaac
     7201 gctggatcat ggtattagca gtagggcagg agatgcagat attctcttct cctaatctga
     7261 aagattggac ctttgaaagt cgtttcggtg aagggcaagg tgcacatggt ggtgtttggg
     7321 aatgtcccga tttgtttgaa cttccagtag aaggtaccaa tgataagaaa tgggtgctgc
     7381 tttgtaattt gaatcccgga ggtcctttcg gaggaagtgc tactcaatat tttgtaggct
     7441 ccttcaacgg aaaagagttt gtaaacgaat tcccttccaa aaccaaatgg atggactggg
     7501 gaaaagacca ttacgccacc gtaacttgga gcgatgctcc cgacaaccgc cgtatcgcta
     7561 tcgcatggat gagcaactgg caatacgcaa acgatgttcc tacctcccaa taccgtagcc
     7621 cgaattccgt tccccgtgac ttgagcctgt ttaccgtaga tggtgagact tacctccaat
     7681 cagctccctc tcccgaactg ctggctttgc gtgatgcttc caagaaacgc tcgttcaaag
     7741 taaacggaac acgcacaatc aaagaaatga ttcccggcaa tgacggtgca tacgaaatag
     7801 aattgacaat cgaaaatcaa cgtgcagacg taatcggctt ccgtctctac aatgacaaag
     7861 gcgaagaagt ggatatgcag tatgacatga aagaaaagaa attctcaatg gatcgtcgca
     7921 agagcggaga ggtcagcttc aatgagaatt tcccgatgtt gacctggaca gcaattgagc
     7981 aaggtggaaa cgaggaacaa ggcggcaaag aggcattgaa actccgtctg tttgtggata
     8041 aatccagtgt ggaagccttt ggaaatggtg gacgttttgt gatgaccaat caggtattcc
     8101 cttcggaacc ttacaatcat atcaactttt acagtaaagg aggagcttat aaagtagatt
     8161 cgtttgtggt tcataagttg aagccataaa caataggcca catgcaactg taaatgttta
     8221 cgcgtcctcg gtaccaacaa atactttcag gacgatgtaa agtcctgaga aatcaaactg
     8281 aatggataga tcataatgct aatcttacac cgtcccacca catttccaat tcagaagctc
     8341 tattgtctat ccagtcatag aactcgtcac cccaagtata atcagccact ccgataccga
     8401 tagctaagac ccaaccgaca tagggaataa aagtaacgcc ataagctaaa cctgctacgg
     8461 caattcttgt acctgccacg gcaaatcgac cattttgcac atcacgcaca acttgatagc
     8521 cagtaagcac cattcctgcc catcccaaag ctttacctac tccacgcact ctactcatac
     8581 ctttagcggg gacttgatag ttccctaaag gcaatcttat aatcggcgga agcgttttgg
     8641 gcgagaaacg catctgctta ggtaagtacg cacctcgatt atccataatc ttctcagccc
     8701 aatttccggc tgtttcagca attccgaatc ctaaccctgc cagtccttcc agattgggtc
     8761 cttccttatt tacagcaggc aggctaccag aatcgcgtac cgctgacacc aagtccgtca
     8821 aactctcata ctttgcctca taagtctttc catttgagtt atccgtggca ctaatcacaa
     8881 tttcgtaccg actgccagtg tcagtaatta caatacttat ctgactatta tccgtgggca
     8941 aatattgctt ttcttggcct aaatttatct cagtgatatt attacctttg tttttaacta
     9001 ttaagtcacg gaccttttcg gaaacttttg ttggatcttg catcagactt ttttcatagg
     9061 catattggga ggtatcgtat ttcctttctg agactatcgt atgaaaactc tcgtccaccg
     9121 aaacattccg agatattgga ctccatccgt tttgatttac cggtagaatt accttgctac
     9181 tcatccaagt atatcccaag ccaactacaa atccttcata gtcatccccc cgttccaaga
     9241 tgacattaaa ttcactttgc gttttatatt catttctata agcgccggca aatgagaact
     9301 gtatagctac aatagccatc acaagcaaag ataaaatttt cttcatgtgt cttttctttt
     9361 atatgtcttt atttcgtctc tatcactgat agggagactg caaagataag aacttttagc
     9421 tttatcttcc aaactttatc cctatcagtg atagagattt ttttgttctt atggtcaagc
     9481 agtactgcgc ttttacacta agtcaattta acaaggcttt cttctcttgg aaagcgggtg
     9541 caaaaaaaga aaaggcgttt tgtttttctt ctttaccttc tttccctttc gctaagagag
     9601 tctgagaaac gatagaaaaa gaaaagcgaa aaaacttccg aaaacatttg gtagttaaaa
     9661 taaaacctct tacctttgca cccgcgcatt ttaaaataaa ataaattatt tatgatatta
     9721 aacgaatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
     9781 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
     9841 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
     9901 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
     9961 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
    10021 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
    10081 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
    10141 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
    10201 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
    10261 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
    10321 aaaacaactt aaatgtgaaa gtgggtctta agatacaagc ggtcggttcg gaaatagaac
    10381 aggcacaagt aaggctgatt gttgcagcca atgcacaaat gctgttccat cactggaaga
    10441 taggcaatta gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
    10501 cactagttct agagcggccg ccaccgcggt ggaggggaat tcccatgtca gccgttaagt
    10561 gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc
    10621 cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt
    10681 tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt
    10741 attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag
    10801 agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa
    10861 catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg
    10921 ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg ttttccgctg
    10981 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
    11041 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
    11101 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
    11161 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg
    11221 ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca
    11281 gcccacctat cacggaattg atccccctcg aattg
//
